The effect of postmortem time, donor age and sex on the generation of neurospheres from adult human retina

Carter, Debra A; Mayer, Eric; Dick, Andrew D.

doi:10.1136/bjo.2007.118141

Cited by 24 publications

(24 citation statements)

References 14 publications

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“…1A, B, and C). In a similar postmortem time interval study, Carter and colleagues have shown reduction in neurosphere cell cultures with extended postmortem time in adult human retina (Carter et al 2007). We observed that the numbers of cells around all the five explants of a given dish were not consistent.…”

Section: Resultsmentioning

confidence: 55%

In vitro culture of fibroblast-like cells from postmortem skin of Katahdin sheep stored at 4°C for different time intervals

Singh

Amoah

et al. 2011

In Vitro Cell.Dev.Biol.-Animal

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Live animals have been produced recently from animal tissues preserved for decades at frozen temperatures with or without cryoprotectants. However, the tissues in these studies were cryopreserved within few hours of animal death to obtain culturable live cells as nuclear donors. How long the tissues can be left unfrozen after animal death, without losing the viability and potential to in vitro culture with comparable morphology and proliferative rate as the fresh tissues, is not completely understood. To understand this phenomenon, ear skin samples from individual sheep (n=3) were procured from slaughter plant and stored at 4 °C. After various intervals (2, 8, 24, 32, 48, and 56 h after slaughter), 2-3 mm(2) pieces (n=10) of skin samples were cultured for 12 d on two dishes (60 mm) for each sheep. Outgrowth of fibroblast-like cells was observed as early as day 4 of culture and was visible on dishes of all time points including 56 h by day 10. The number of outgrowing cells decreased with increasing time interval between animal slaughter and culture initiation. Secondary cultures were successfully established for all the time points. All cultures proliferated well and were apparently normal. Passage 2 cultures of 2 h and 56 h interval for one of the three sheep were compared for their growth pattern and proliferation rates. The population doubling time of 2 h and 56 h intervals was 33.12 and 34.8 h, respectively, and both the lines exhibited similar cell morphology and an "S"-shaped growth curve. These results suggest that skin tissues of sheep and perhaps other animal species with superior traits are effectively preserved at cellular level at least for 56 h at normal refrigerating conditions, without need of complicated cryopreservatives/cryotanks that are usually not available at small farms.

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Section: Resultsmentioning

confidence: 55%

In vitro culture of fibroblast-like cells from postmortem skin of Katahdin sheep stored at 4°C for different time intervals

Singh

Amoah

et al. 2011

In Vitro Cell.Dev.Biol.-Animal

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“…The gestational age of each fetus was determined using the last menstruation date and fetal foot length (Merz et al, 2000;Drey et al, 2005). Postmortem times of less than 1 h were used because they do not alter the ability of progenitor cells to survive in culture (Carter et al, 2007).…”

Section: Cell Isolation and Culturementioning

confidence: 99%

c-Kit+ cells isolated from human fetal retinas represent a new population of retinal progenitor cells

Zhou

Peng

et al. 2015

Journal of Cell Science

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“…Following our protocol that isolates retinal progenitor cells from adult human retina, 44 we further noted that with exogenous addition of pro-inflammatory cytokine IL-6, the ability to generate neurospheres, a hallmark of cell turnover and differentiation of progenitor cells (see above), is diminished. This effect was reversed when IL-6 was neutralised effectively with a specific anti-IL-6 mAb.…”

Section: Microglia Influence Retinal Progenitor Cell Functionmentioning

confidence: 99%

“…The observation of nestin-positive cells in CMZ as well as in radial cells within the retina infers the presence of progenitor cell populations within the retina as well as that of cells capable of directing migration of cells within the tissue towards cell renewal. 44,45 Others have also described from an immortalised Muller glia cell line the potential of cell depots with progenitor cell potential. 46,47 Further, our initial observations demonstrated that cells from epiretinal membranes of surgical specimens expressed nestin.…”

Section: Retinal Progenitor Cellsmentioning

confidence: 99%

Influence of microglia on retinal progenitor cell turnover and cell replacement

Dick

2008

Eye

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The effect of postmortem time, donor age and sex on the generation of neurospheres from adult human retina

Cited by 24 publications

References 14 publications

In vitro culture of fibroblast-like cells from postmortem skin of Katahdin sheep stored at 4°C for different time intervals

In vitro culture of fibroblast-like cells from postmortem skin of Katahdin sheep stored at 4°C for different time intervals

c-Kit+ cells isolated from human fetal retinas represent a new population of retinal progenitor cells

Influence of microglia on retinal progenitor cell turnover and cell replacement

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