The effect of polystyrene foam in different doses on the blood parameters and relative mass of internal organs of white mice

Modern aspects of the pathogenesis of acute inflammation of the peritoneum that is concurrent with diabetes involves analysis of metabolic mechanisms, in particular peroxidaton of proteins – antioxidant defense. Therefore, the objective of our study was to examine the interrelation between the processes of free-radical oxidation of proteins and antioxidant system in the dynamics of development of acute generalized peritonitis against the background of streptozotocin-induced diabetes. The study was performed on 56 non-linear white mature male rats. Diabetes mellitus was modeled by a single intraperitoneal injection of streptozotocin (60 mg/kg). On the 14th day of the development of streptozotocin-induced diabetes, we injected 10% filtrated faeces suspension (0.5 mL) into the abdominal cavity, thus initiating acute generalized peritonitis. Oxidative modification of proteins in blood serum was studied using the I. F. Meshchyshen’s method and the condition of antioxidant protection was monitored according to the activities of superoxide dismutase, catalase, content of reduced glutathione, and the level of ceruloplasmin. The study of the parameters of free-radical oxidation of proteins and study of the condition of antioxidant system in blood of the rats with experimental acute generalized peritonitis against the background of streptozotocin-induced diabetes demonstrated that oxidative protein modification grew, while the parameters of the activity of antioxidant system were being inhibited, depending on the stage of acute inflammation of the peritoneum. We determined inverse correlation relationships between the products of free-radical oxidation of proteins and parameters of antioxidant system on the third and on the seventh days of modelling of combined pathology. Manifestation of acute generalized peritonitis concurring with streptozotocin-induced diabetes was accompanied by a gradual accumulation of the products of free-radical oxidation of proteins and exhaustion of the antioxidant defense during all stages of the development of acute inflammation of the peritoneum, peaking on the seventh day after administration of faecal suspension (terminal stage of peritonitis). The observed inverse correlations between the levels of oxidative modification of proteins and the activity of superoxide dismutase, catalase, reduced glutathione, and ceruloplasmin on the third and on the seventh days of modelling of combined pathology indicate a predictive role of the processes of free-radical oxidation of proteins in exhaustion of antioxidant-defense resources.

Section: Discussionmentioning

confidence: 99%

Oxidative modification of proteins and antioxidant status in blood of the rats with experimental acute generalized peritonitis against the background of streptozotocin-induced diabetes

Verveha,

Gutyj,

Lishchuk

et al. 2023

“…Colonies were counted in all media dishes. CFU/g (colony forming units per 1 g of intestinal content) was calculated and multiplied by the appropriate dilution (Bilan et al, 2022b). Anaerobic conditions for bifidobacteria, lactobacilli, clostridia were achieved in anaerostats (7 L) using GENbox anaer anaeropackets (Biomerieux, France).…”

Section: Methodsmentioning

confidence: 99%

Impacts on gut microbiota of rats with high-fat diet supplemented by herbs of Melissa officinalis, Lavandula angustifolia and Salvia officinalis

Bilan,

Lieshchova,

Brygadyrenko

2023

Self Cite

Medicinal plants are used in traditional, folk medicine and veterinary practice to treat and prevent exacerbations of chronic diseases, diseases of the cardiovascular and nervous systems, respiratory and digestive organs, liver, bile ducts, kidneys, urinary tract, to regulate metabolism and to boost immunity, etc. The therapeutic effect on the body is exerted by biologically active substances (alkaloids, glycosides, tannins, essential oils, and others) that are present in various parts of plants. Understanding the issue of interaction between the intestinal microbiota and drugs based on medicinal plants will contribute to the development of treatment protocols for various diseases, including chronic ones, by the normalization of impaired functions due to plant origin substances. In this study, we determined the effect of Melissa officinalis, Lavandula angustifolia, Salvia officinalis on the intestinal microbiota of white rats fed a high-fat diet. The addition of 5% crushed young dry shoots of S. officinalis, L. angustifolia, M. officinalis to the high-fat diet of laboratory rats significantly changed the quantitative ratio of Escherichia coli with normal and altered enzymatic properties in the intestinal content. The number of typical E. coli in rats fed with S. officinalis and L. angustifolia decreased by 1.7 and 1.6 times; non-lactose fermenting form of E. coli, on the contrary, increased by 1.8–2.1 times in rats fed with any of the medicinal plants compared to the control. Amid the addition of medicinal plants to the diet, it was not possible to isolate opportunistic enterobacteria of the genus Citrobacter, however, an increase in the number of the genus Candida fungi was observed.

“…The main structural components of the lipid bilayer in cell membranes, as is known, are phospholipids (about 65% from the lipid component) (Gryshchenko et al, 2016;Gryshchenko et al, 2019;Dajani & Popovic, 2020). Thus, the functioning of external and internal membrane systems depends on the integrity of their phospholipid structures (Acoba et al, 2021) Therefore, the completeness of cellular metabolic processes and their violation in the development of pathology is determined by the structural and functional state of membrane systems (Doralicia et al, 2019;Bilan et al, 2022;, 2023a, 2023b.…”

Section: Introductionmentioning

confidence: 99%

Lipid composition of blood plasma and epithelium of the jejunal mucosa in calves with dyspepsia and its correction

Gryshchenko,

Danchenko,

Tkachuk

et al. 2023

Self Cite

Enteropathology in newborn ruminants significantly affects the functional formation of the digestive tract, which induces complications in other organs and systems, reducing the resistance and productivity in recovered animals. It is also characterized by metabolic disorders, particularly lipids, which adversely affects the regenerative processes at the cellular level. The material for the study was 2-day-old calves, from which 3 groups of 6 animals each were formed. 1st group – control was made up of clinically healthy animals, 2nd group – calves with toxic dyspepsia which received traditional treatment and 3rd group – calves which were additionally orally administered a liposomal form of a 1% solution of BAD "FLP-MD". The lipid composition of blood plasma and the epithelium of the mucous membrane of the jejunum of calves was studied by the method of thin-layer chromatography on standard Silufol plates. The lipidogram of blood plasma in 30-day-old calves recovered from dyspepsia under traditional treatment regimen was characterized by dyslipidemia, which was manifested by an increase in total lipid content by 10.0% amid hypercholesterolemia and hypertriacylglycerolemia and a decrease in free fatty acids by 24.6% and phospholipids by 11.8%. Also, phosphatidylcholine and sphingomyelin content decreasesd in the blood plasma. In epithelium of the jejunal mucosa, total lipid content decreased due to total cholesterol, triacylglycerols, and phospholipids. The restoration of total lipid content and individual fractions was found in blood plasma of calves that received additionally phospholipid-containing bio-additive "FLP-MD". Among the individual phospholipids phosphatidylcholine, phosphatidylethanolamine and phosphatidylserine prevailed. In these calves, most of the lipid components in the epithelium of the jejunal mucosa were stabilized. In the future, we plan to investigate the content of total lipids in the blood and mucous membrane of the small intestine of calves.