The Effect of Polyoxyethylene–Polyoxypropylene Triblock Copolymers on the Loading Degree of Poly-(Lactic-co-Glycolic Acid) Copolymer-Based Microparticles Containing Chlorin e6 and Ethidium Bromide in Mesenchymal Stem Cells

Temnov, A. A.; Sklifas, A. N.; Kukushkin, N. I.; Krechetov, S. P.; Gorina, E. V.; Астрелина, Т. А.; Usupzhanova, D.; Suchkova, Yu.B.; Kobzeva, I. V.; Самойлов, А. С.

doi:10.1134/s0006350919020179

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“…22,23 The inclusion of RC into MPs is considered as a promising trend for improving the efficiency of its use for PDT and fluorescence diagnostics. 24 The preparation of such MPs should potentiate intracellular accumulation and deposition of RC, as well as prolongation of its effect. This will allow further localize the effect of this PS and to extend the duration of the therapeutic window for PDT.…”

Section: Introductionmentioning

confidence: 99%

Radachlorin-Containing Microparticles for Photodynamic Therapy

et al. 2020

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Purpose: Reducing the undesirable systemic effect of photodynamic therapy (PDT) can be achieved by incorporating a photosensitizer in microparticles (MPs). This study is devoted to the preparation of biocompatible biodegradable MPs with the inclusion of the natural photosensitizer Radachlorin (RС) and an assessment of the possibility of their use for PDT. Methods: RC-containing MPs (RС MPs) with poly(lactic-co-glycolic acid) copolymer (PLGA) matrix were prepared by a double emulsion solvent evaporation methods. The size and morphology of RC MPs were surveyed using scanning electron microscopy, confocal laser scanning microscopy, and dynamic light scattering. The content of RC, its release from RC MPs, and singlet oxygen generation were evaluated by the optical spectroscopy. Cellular uptake and cytotoxic photodynamic effect of RC MPs were investigated with in vitro assays. Results: The average diameter of the prepared RC MPs was about 2-3 μm. The RC MPsprepared by the water/oil/oil method had a significantly higher inclusion of RC (1.74 μg/mg) then RC MPs prepared by the water/oil/water method (0.089 μg/mg). Exposure of the prepared RC MPs to PDT light radiation was accompanied by the singlet oxygen generation and a cytotoxic effect for tumor cells. The release of the RC from the RC MPs was prolonged and lasted at least two weeks. Conclusion: PLGA RC MPs were found to cause a photoactivated cytotoxic effect for tumor cells and can be used for local application in PDT of tumors.

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Section: Introductionmentioning

confidence: 99%