The effect of polychlorinated biphenyls (PCBs) on the membrane lipids of Pseudomonas stutzeri

Zorádová, Slavomíra; Dudášová, Hana; Lukáčová, Lucia; Dercová, Katarı́na; Čertı́k, Milan

doi:10.1016/j.ibiod.2011.03.012

Cited by 26 publications

(24 citation statements)

References 19 publications

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“…This parameter achieved half value of control accumulation. These results are in agreement with those reported by Zorádová et al (2011) and Zorádová-Murínová et al (2012) where signifi cant reduction of growth of different bacterial strains was observed when persistent organic pollutants were added at the time of inoculation. Opposite effect was observed by Dercová et al (2004).…”

Section: Effect Of Pcbs and 3-cba On The Bacterial Growth And Lipid Asupporting

confidence: 93%

“…Although, many bacteria have the ability to metabolize PCBs, high concentrations of these chemicals in the environment inhibit cells survival and their ability to metabolize PCBs. If bacterial strains wanted to survive, they had to develop effi cient adaptation mechanisms in the hostile environment (Čertík and Breierová 2002;Zorádová et al 2011). Such resistant bacteria may play a major role in bioremediation technologies.…”

Section: Introductionmentioning

confidence: 99%

“…Since fatty acids are the major constituents of membrane phospholipids, modulation of number and position of double bonds of acyl chains by specifi c fatty acid desaturases plays crucial role in preserving a suitable dynamic state of the bilayer during environmental impact (Šajbidor, 1997). Polychlorinated biphenyls affect the saturation of membrane fatty acids of various bacterial strains by increasing the saturation of fatty acids (Zorádová et al, 2011). A correlation between an increase in the degree of saturation of membrane fatty acids and increased tolerance towards the toxic compounds in phenol-degrading strain P. putida P8 was desribed (Heipieper et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

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Bacterial cell membrane adaptation responses on stress caused with the environmental pollutants

Murínová¹,

Dercová²

2013

Acta Chimica Slovaca

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The effect of polychlorinated biphenyls on biomass production, lipid accumulation, and on the fatty acid profi le of the major membrane lipids of Alcaligenes xylosoxidans isolated from a soil long-term contaminated with polychlorinated biphenyls was examined. The lowest bacterial growth was observed in the presence of biphenyl and polychlorinated biphenyls. On the other hand, the highest growth stimulation was observed in the presence polychlorinated biphenyls. Higher growth ability was observed when polychlorinated biphenyls or 3-chlorobenzoic acid were added after three days of cultivation. Higher lipid accumulation corresponded with lower growth ability. The importance of time when bacterial strain meets polychlorinated biphenyls or 3-chlorobenzoic acid should be emphasized. The addition of toxic organic compound later in stationary growth phase led to better survival and evoked lower membrane adaptation. However, polychlorinated biphenyls or 3-chlorobenzoic acid amendment at the beginning of cultivation stimulated adaptation responses as membrane saturation, cis to trans isomerisation, and branched fatty acids alteration and decreased growth ability. Palmitic acid and 17-cyclopropyl fatty acid were observed as prevalent in the bacterial membrane as well as in the total lipids.

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Section: Effect Of Pcbs and 3-cba On The Bacterial Growth And Lipid Asupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Bacterial cell membrane adaptation responses on stress caused with the environmental pollutants

Murínová¹,

Dercová²

2013

Acta Chimica Slovaca

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“…Upon cultivation, bacterial biomass was centrifuged, dried and weighted according to Zorádová et al (2011). Lipids from the biomass were extracted using chloroform : methanol mixture and subsequently fractionated by thin-layer chromatography with the solvent system chloroform : acetone : methanol : acetic acid : water to separate polar lipids (Čertík & Shimizu 2000).…”

Section: Lipid Extraction and Fractionationmentioning

confidence: 99%

“…The identification of fatty acid methylester peaks were performed by comparison to the standards of C4-C24 fatty acid methylesters mixture (Supelco, USA). The index of fatty acid unsaturation was calculated as ∆ mol −1 according to Zorádová et al (2011).…”

Section: Analysis Of Fatty Acidsmentioning

confidence: 99%

The adaptation responses of bacterial cytoplasmic membrane fluidity in the presence of environmental stress factors — polychlorinated biphenyls and 3-chlorobenzoic acid

et al. 2014

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Only bacteria sufficiently resistant to the toxic compounds in their environment can be used for the efficient biodegradation process in order to eliminate a widespread contamination by polychlorinated biphenyls (PCBs). The presence of PCBs results in bacterial controlled rigidification of cytoplasmic membrane. The four bacterial isolates from long-term PCB-contaminated soil (Alcaligenes xylosoxidans, Pseudomonas stutzeri) and sediment (Ochrobactrum anthropi, Pseudomonas veronii) have been used to select the strain most adapted to the PCBs, i.e. with efficient changes in the membrane phospholipid fatty acids. PCBs and their toxic degradation products -the 3-chlorobenzoic acids (3-CBA as the most toxic one) -were added separately to the liquid medium with glucose in two experimental sets: at lag phase and in stationary phase of bacterial growth in order to evaluate the effects of chemicals to cytoplasmic membrane. The main parameter -the changes in fatty acids composition (in the total lipids and the main membrane phospholipid phosphatidyletanolamine) were studied. 3-CBA caused growth inhibition when added at lag phase. However, when added during the stationary growth, inhibition was not observed. Similarly, after addition of PCBs to the stationary growth culture, inhibition of growth was not observed with all tested strains (except for P. stutzeri). This fact indicates the importance of time contact of bacteria during growth phase with xenobiotics. O. anthropi and A. xylosoxidans appeared to be the most adapted to the presence of PCBs (with sufficient membrane adaptation), active under the adverse conditions, and able to survive in the contaminated environment.

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Membrane Composition and Modifications in Response to Aromatic Hydrocarbons in Gram-Negative Bacteria

Ortega¹,

Segura²,

Bernal³

et al. 2017

Cellular Ecophysiology of Microbe

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The effect of polychlorinated biphenyls (PCBs) on the membrane lipids of Pseudomonas stutzeri

Cited by 26 publications

References 19 publications

Bacterial cell membrane adaptation responses on stress caused with the environmental pollutants

Bacterial cell membrane adaptation responses on stress caused with the environmental pollutants

The adaptation responses of bacterial cytoplasmic membrane fluidity in the presence of environmental stress factors — polychlorinated biphenyls and 3-chlorobenzoic acid

Membrane Composition and Modifications in Response to Aromatic Hydrocarbons in Gram-Negative Bacteria

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