The effect of poly-β-hydroxybutyrate on the performance of Chinese mitten crab (Eriocheir sinensisMilne-Edwards) zoea larvae

Abstract

This study investigated the protective effect of polyb-hydroxybutyrate (PHB) on Chinese mitten crab, Eriocheir sinensis, zoea larvae challenged with pathogenic Vibrio anguillarum. PHB was delivered to the crab larvae through rotifer and Artemia bioencapsulation. Zoea 3 larvae were challenged with V. anguillarum at a final concentration of 10 5 CFU mL )1. PHB-enriched rotifers and Artemia nauplii were added to the culture water 24 h prior to, upon and 24 h after challenge. The results confirmed that PHB could enhance the survival and growth of unexposed E. sinensis larvae. Moreover, PHB protected larvae from the pathogen as the larvae fed PHB-enriched live food showed the highest survival and development rate in all challenged groups (P < 0.05). Furthermore, larval performance was the best when PHB was delivered to the larvae 24 h before challenge (P < 0.05). In conclusion, our results indicate that PHB can be used as part of an effective strategy to protect E. sinensis larvae from V. anguillarum resulting in higher survival and better growth, especially when applied before the challenge.

Effect of poly-β-hydroxybutyrate on growth, enzyme activity and intestinal microbial community of Chinese mitten crab,Eriocheir sinensis(Milne-Edwards) juveniles

“…In the current study, 1% and 3% PHB supplementation in the feed of juvenile Chinese mitten crab resulted in faster moulting and correspondingly better body weight gain. This confirms the previous observations of PHB containing diets benefiting the moulting and survival of Chinese mitten crab zoeal larvae (Sui et al 2012(Sui et al , 2014. These observations are also in accordance with the results reported on fish such as European sea bass , Siberian sturgeon (Najdegerami et al 2012), and other crustacean species such as the giant freshwater prawn (Nhan et al 2010).…”

“…This confirms the previous observations of PHB containing diets benefiting the moulting and survival of Chinese mitten crab zoeal larvae (Sui et al . , ). These observations are also in accordance with the results reported on fish such as European sea bass (De Schryver et al .…”

“…It has been reported that PHB enrichment of formulated feed or live food (rotifer and Artemia ) improves the survival and growth of European sea bass ( Dicentrarchus labrax ) juveniles (De Schryver, Sinha, Kunwar, Baruah, Verstraete, Boon, De Boeck & Bossier ), Siberian sturgeon ( Acipenser Baerii ) fingerlings (Najdegerami, Tiet, Defoirdt, Marzorati, Sorgeloos, Boon & Bossier ) and giant fresh water prawn ( Macrobrachium rosenbergii ) larvae (Nhan, Wille, De Schryver, Defoirdt, Bossier & Sorgeloos ). Our recent studies have also shown that PHB‐enriched rotifers and Artemia nauplii significantly improve the moulting, survival and tolerance to vibriosis challenge of Chinese mitten crab zoeal larvae (Sui, Cai, Sun, Wille & Bossier ; Sui, Liu, Sun, Wille, Bossier & De Schryver ).…”

Short-chain fatty acids as feed supplements for sustainable aquaculture: an updated view

“…() reported that feeding the giant freshwater prawn ( Macrobrachium rosenbergii ) larvae with poly β‐hydroxybutyrate (PHB)‐containing Artemia nauplii significantly increased survival and development of the larvae. Sui, Liu, Sun, Wille, Bossier and Schryver () reported that bio‐encapsulation of PHB into rotifers and Artemia was an efficient approach to deliver PHB to the Chinese mitten crab ( Eriocheir sinensis ) larvae and resulted in a significantly enhanced survival, development rate and osmotic tolerance. The addition of commercial PHB particles (average diameter 30 μm) or PHB‐containing bacteria protects Artemia franciscana from pathogenic Vibrio campbellii (Defoirdt, Halet, Vervaeren, Boon, Van de Wiele, Sorgeloos, Bossier & Verstraete ; Halet et al .…”

Poly-beta-hydroxybutyrate-enrichedArtemiasp. for giant tiger prawnPenaeus monodonlarviculture

“…PHB and/or its degradation product β‐HB can probably also be used as an energy source by crustaceans (Sui et al . ). Organic acids, mainly butyric acid, have also been mentioned as typical energy sources for the growth of intestinal epithelial cells (Biagi et al .…”