The effect of poly(d,l-lactide-co-glycolide) microparticles with polyelectrolyte self-assembled multilayer surfaces on the cross-presentation of exogenous antigens

Yang, Yao-Hsu; Hsu, Paul Yueh-Jen

doi:10.1016/j.biomaterials.2008.02.015

Cited by 65 publications

(51 citation statements)

References 47 publications

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“…The main evidence was the increased expression of CD80/CD86 on dendritic cell surfaces. 24,25 However, our findings were different. The PEI-OVA 0.04 nanoparticles had a negative surface potential, and there was no significant upregulation of CD80/86 expression on dendritic cells.…”

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confidence: 84%

Improved antigen cross-presentation by polyethyleneimine-based nanoparticles

Chen¹,

Li²,

Huang³

et al. 2011

IJN

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Purpose In the development of therapeutic vaccines against cancer, it is important to design strategies for antigen cross-presentation to stimulate cell-mediated immune responses against tumor antigens. Methods We developed a polyethyleneimine (PEI)-based protein antigen delivery system to promote cross-presentation through the major histocompatibility complex (MHC) I pathway using ovalbumin (OVA) as a model antigen. PEIs formed nanoparticles with OVA by electrostatic interactions, as demonstrated by electrophoresis analysis, scanning electron microscopy, and photon correlation spectroscopy analysis. Results The nanoparticles were used to stimulate mouse bone marrow-derived dendritic cells in vitro and resulted in significantly more OVA 257–264 /MHC I complex presentation on dendritic cell surfaces. The activated dendritic cells interacted specifically with RF33.70 to stimulate interleukin-2 secretion. The cross-presentation promoting effect was more prominent in dendritic cells that had been cultured for longer periods of time (13 days). Further studies comparing the antigen presentation efficacies by other polyanionic agents, such as PLL or lysosomotropic agents, suggested that the unique “proton sponge effect” of PEI facilitated antigen escape from the endosome toward the MHC I pathway. Conclusion Such a PEI-based nanoparticle system may have the potential to be developed into an effective therapeutic vaccine delivery system.

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confidence: 84%

Improved antigen cross-presentation by polyethyleneimine-based nanoparticles

Chen¹,

Li²,

Huang³

et al. 2011

IJN

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“…30 Amine functionalized QDs (Em: 655 nm) were covalently conjugated with carboxyl group terminals on the surface of PLGA NPs using EDC cross linker reagent. The synthetic methodology of NPs is shown in Scheme II.…”

Section: Resultsmentioning

confidence: 99%

Stabilizer‐Free Poly(lactide‐co‐glycolide) Nanoparticles Conjugated with Quantum Dots as a Potential Carrier Applied in Human Mesenchymal Stem Cells

Kuo

Hwang

Sei

et al. 2009

J Chinese Chemical Soc

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We report that human mesenchymal stem cells (hMSCs) were successfully labeled with poly(lactideco-glycolide) nanoparticles (PLGA NPs) surface-conjugated quantum dots (QDs) (PLGA-QD NPs) via endocytosis pathway. These NPs were not toxicity even treated with PLGA-QD NPs at high concentrations for at least four weeks. Besides, PLGA-QD NPs-labeled hMSCs did not change their proliferation and differentiation capability toward the cell fates of adipocytes, osteocytes, and chrondrocytes. It's known that PLGA has been widely employed to act as delivery carrier which encapsulates drugs and releases them under a controlled way. Currently, we have also demonstrated that FITC-loaded PLGA-QD NPs degraded in hMSCs to achieve intracellular release of FITC. The aim of this research is to investigate viability, proliferation and differentiation capability and the potential for gene delivery of MSCs labeled with PLGA-QD NPs. In addition to PLGA-QD NPs, QDs alone were used to serve as a control set for comparison.

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“…The drug load can be easily tuned with the number of layers incorporated [140]. The LbL technique has been used to encapsulate drugs onto microsphere substrates [141][142][143]. In one example, a multilayered polyelectrolyte delivery system was constructed on the surface of PLGA microspheres using LbL [138].…”

Section: Drug Releasing Microspheres As Cell Carriersmentioning

confidence: 99%

Nanostructured Injectable Cell Microcarriers for Tissue Regeneration

Zhang

Eyster

Peter

2016

Nanomedicine (Lond.)

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Biodegradable polymer microspheres have emerged as cell carriers for the regeneration and repair of irregularly shaped tissue defects due to their injectability, controllable biodegradability and capacity for drug incorporation and release. Notably, recent advances in nanotechnology allowed the manipulation of the physical and chemical properties of the microspheres at the nanoscale, creating nanostructured microspheres mimicking the composition and/or structure of natural extracellular matrix. These nanostructured microspheres, including nanocomposite microspheres and nanofibrous microspheres, have been employed as cell carriers for tissue regeneration. They enhance cell attachment and proliferation, promote positive cell-carrier interactions and facilitate stem cell differentiation for target tissue regeneration. This review highlights the recent advances in nanostructured microspheres that are employed as injectable, biomimetic and cell-instructive cell carriers.

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The effect of poly(d,l-lactide-co-glycolide) microparticles with polyelectrolyte self-assembled multilayer surfaces on the cross-presentation of exogenous antigens

Cited by 65 publications

References 47 publications

Improved antigen cross-presentation by polyethyleneimine-based nanoparticles

Improved antigen cross-presentation by polyethyleneimine-based nanoparticles

Stabilizer‐Free Poly(lactide‐co‐glycolide) Nanoparticles Conjugated with Quantum Dots as a Potential Carrier Applied in Human Mesenchymal Stem Cells

Nanostructured Injectable Cell Microcarriers for Tissue Regeneration

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