The effect of platelet-rich plasma on early and late bone healing using a mixture of particulate autogenous cancellous bone and Bio-Oss®: an experimental study in goats

Mooren, R.E.C.M.; Dankers, Anita C. A.; Merkx, Matthias A.W.; Bronkhorst, Ewald M.; Jansen, John A.; Stoelinga, Paul J.W.

doi:10.1016/j.ijom.2009.12.019

Cited by 50 publications

(29 citation statements)

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“…Various species, e.g., NWR, Japanese white rabbits [Ohba et al, 2012a], Mongrel dogs [Choi et al, 2004], and Danish Landrace pigs [Li et al, 2004] weight of the NWR have also differed [Kasten et al, 2008;Batista et al, 2011;Parizi et al, 2012;El Backly et al, 2013;Lin et al, 2013]. Baseline platelet counts vary markedly within and among species [ Table 6 in Kahn et al, 2006], and the difference in the metabolism of small and large animals may influence the rate of bone healing [Mooren et al, 2010]. A critical defect is more proper for evaluating the bone regeneration potential of PRP and the eventual clinical application of preclinical results, particularly in weight-bearing bones.…”

Section: Discussionmentioning

confidence: 99%

“…Allogeneic, pooled, or xenogenic PRP has been used instead of autologous PRP in some preclinical studies [Ohba et al, 2012a, b;Parizi et al, 2012;Shafiei-Sarvestani et al, 2012;El Backly et al, 2013], and human PRP has been reported to be more potent than animal-derived PRP for bone regeneration [Mooren et al, 2010]. An in vitro study demonstrated that the growth factor concentration is highest in human PRP, followed by goat and rat PRP [van den Dolder et al, 2006].…”

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confidence: 99%

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The Effect of Autologous Platelet-Rich Plasma on Bone Regeneration by Autologous Mesenchymal Stem Cells Loaded onto Allogeneic Cancellous Bone Granules

Joo

Chung

Shin

et al. 2017

Cells Tissues Organs

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To develop a clinically effective bone regeneration strategy, we compared bone regeneration using allogeneic cancellous bone granule scaffolds loaded with autologous bone marrow-derived mesenchymal stem cells (BM-MSC) with or without autologous platelet-rich plasma (PRP). Critical-sized segmental bone defects were made at the mid-shaft of both radiuses in 41 New Zealand White rabbits. Small-sized allogeneic cancellous bone granules (300-700 μm in diameter) loaded with BM-MSC were implanted on one side, and PRP was added. On the other side, autologous BM-MSC loaded onto allogeneic cancellous granules were grafted as a control. Bone regeneration was assessed by radiographic evaluation at 4, 8, and 16 weeks postimplantation and by micro-computed tomography (micro-CT) and histological evaluation of the retrieved specimens at 8 and 16 weeks. The experimental group did not show significantly higher bone quantity indices than the control group at any time point. Micro-CT analysis revealed that both groups had similar mean total volumes, surface areas, and other parameters at 8 and 16 weeks. Histological evaluation of 8- and 16-week specimens also showed a similar progression of new bone formation and maturation. In this experiment using a contralateral control group in the same individual, an initial single addition of PRP in allogeneic cancellous bone granules loaded with BM-MSC for critical-sized bone defects in the weight-bearing area did not induce a consequent difference in bone healing. Further research into the optimal preparation and application of PRP is necessary. Furthermore, studies involving a greater number of subjects and larger experimental animals could determine the clinical relevance of PRP treatment.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

The Effect of Autologous Platelet-Rich Plasma on Bone Regeneration by Autologous Mesenchymal Stem Cells Loaded onto Allogeneic Cancellous Bone Granules

Joo

Chung

Shin

et al. 2017

Cells Tissues Organs

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“…However, others could not confirm enhancement effect of PRP on bone healing when combined with other implants (Mooren et al, 2010). Additionally, more recent published study stated that PRP alone or combined with bovine inorganic bone had not exert any significant effect on bone healing of bone cavities created in dogs mandibles (Bassi and Carvalho, 2011).…”

Section: Discussionmentioning

confidence: 98%

Novel Practical Insight into Application of Platelet-Rich Plasma in Combination with Bioresorbable Silicate 45S5 Bioactive Glass for Bone Defect Repair: an Experimental Study in a Rabbit Model

Samy¹

2014

Adv. Anim. Vet. Sci.

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| This study was aimed to verify the individual or combining effect of platelet-rich plasma (PRP) and unique biodegradable silicate 45S5 bioactive glass (BG) scaffold on regeneration of metaphyseal tibia defect in a rabbit model. Thirty-six animals were allocated into three groups (n=12 each). In each group, during surgery, right-sided defects were filled with BG, PRP or BG/PRP (1:1), while left-sided defects were served as a control. To visualize and trace defects healing, macroscopic, radiologic and histological examinations were performed at different time points. Bone density/radiopacity and macroscopic (defect size) values were collected and analyzed statistically. Histomorphometric quantitative analysis of H&E stained sections to quantify the percent bone formation at 6 and 8 weeks after implantation was also performed. The gained results showed a significant increase of bone regeneration by 60 days in both BG and BG/PRP-groups (P<0.05 and P<0.001, respectively). Whereas, the healing pattern created by PRP/BG was better comparing to that of BG alone. We noticed unexpectedly incomplete defect closure in PRP-group by 60 days, even though and interestingly, PRP alone accelerated bone radiopacity and new osseous formation significantly only at early stages of healing period (P<0.01). Histomorphometric analyzed data indicated an increasing bone formation over time in all treated defects except PRP during 8 weeks. In conclusion, PRP alone does not provide an appreciated healing effect; however, in future, use of PRP combined with 45S5 BG might be clinically beneficial for proper bone regeneration as safe and appropriate biomaterial.

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“…Jeong et al (2014) realizaron cirugías de elevación de seno maxilar rellenando exclusivamente con PRF, describiendo baja predictibilidad y limitada respuesta ósea; los autores concluyeron que el uso de PRF seria poco confiable para cirugías de reconstrucción ósea. Mooren et al (2010), también concluyeron que utilizando matriz bovina con hueso autógeno no presentaban diferencias cuando el PRP fue incorporado en uno de los grupos; esta ausencia de diferencias se mantuvo con las mismas limitaciones tanto al inicio como al final de las evaluaciones.…”

Section: Discussionunclassified

Respuesta Tisular en Defectos Tratados con Matriz Ósea de Origen Bovino o Humano

et al. 2016

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RESUMEN: El objetivo de esta investigación fue comparar la respuesta tisular en defectos tratados con matriz óseo de origen bovino y humano. Se realizó un estudio descriptivo con 12 conejos en los cuales se confeccionaron dos defectos en la calota craneana de acuerdo a procesos convencionales. Cada defecto fue aleatoriamente rellenado con matriz ósea bovina o con matriz ósea humana; se realizaron evaluaciones histológicas a las 7 y 15 semanas de realizada la cirugía (6 animales en cada grupo). A las 7 semanas se identificó un leve infiltrado inflamatorio y claras diferencias entre el hueso periférico y el sitio injertado, mientras que a las 15 semanas se observo la persistencia de algunas partículas injertadas y la presencia de hueso nuevo formado en el defecto; no se logró identificar diferencias en la secuencia de reparación o retrasos en la misma con ninguno de los dos materiales. Se concluye que ambos materiales son útiles en la regeneración ósea en el presente modelo experimental.PALABRAS CLAVE: Injerto óseo; Biomaterial; Hueso bovino; Hueso humano. INTRODUCCIÓNLa regeneración ósea en implantología oral es constantemente utilizada para la instalación de implantes en condiciones funcionales y estéticas adecuadas. La variedad de procedimientos y materiales hacen que la planificación y conformación de la secuencia quirúrgica sea compleja toda vez que la selección de los materiales puede limitar los resultados esperados (Olate et al., 2007).Históricamente, el hueso autógeno ha mostrado los mejores resultados en términos de integración del injerto y vitalidad del mismo; sin embargo, esta condición implica una mayor morbilidad y riesgos quirúrgicos debido a la necesidad de obtener hueso del mismo paciente (Noia et al., 2011;Rocha et al., 2010). Los materiales para relleno óseo de origen animal y de origen humano han limitado esta complicación, aunque han sido utilizado con relativo éxito. Variables como el tipo de defecto, el volumen de relleno, el vehículo de aplicación, el empleo de membranas biológicas y la mezcla con otros materiales parecen influenciar en los resultados finales Olate et al., 2013; Cantín et al., 2015) El hueso sintético, ha avanzado sostenidamente en el tiempo demostrando cierta efectividad en algunos estudios en modelo animal (de Oliveira et al., 2014). El hueso bovino y el hueso humano también se encuentran en condiciones semejantes. La matriz de hueso bovino ha sido analizado por nuestro grupo demostrando éxito en estudios en modelo animal, aunque aun existen variaciones que requieren nuevos análisis . Comparaciones entre hueso bovino y hueso humano en la respuesta ósea han mostrado resultados controversiales.El objetivo de este estudio es comparar la secuencia y modelo de reparación ósea entre un relleno óseo de origen bovino y otro relleno óseo de origen humano en un modelo experimental. MATERIAL Y MÉTODOSe utilizaron 12 conejos albinos de raza New Zealand, machos, con un peso promedio de 3.0 Kg y con edad de entre 3 y 6 meses. Todos los animales se mantuvieron con dieta de aliment...

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The effect of platelet-rich plasma on early and late bone healing using a mixture of particulate autogenous cancellous bone and Bio-Oss®: an experimental study in goats

Cited by 50 publications

References 35 publications

The Effect of Autologous Platelet-Rich Plasma on Bone Regeneration by Autologous Mesenchymal Stem Cells Loaded onto Allogeneic Cancellous Bone Granules

The Effect of Autologous Platelet-Rich Plasma on Bone Regeneration by Autologous Mesenchymal Stem Cells Loaded onto Allogeneic Cancellous Bone Granules

Novel Practical Insight into Application of Platelet-Rich Plasma in Combination with Bioresorbable Silicate 45S5 Bioactive Glass for Bone Defect Repair: an Experimental Study in a Rabbit Model

Respuesta Tisular en Defectos Tratados con Matriz Ósea de Origen Bovino o Humano

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