The effect of platelet-rich plasma (PRP) combined with a bone allograft on human periodontal ligament (PDL) cells

Pantou, Agni; Markopoulou, C E; Dereka, Xanthippi; Vavouraki, H.; Mamalis, Anastasios; Vrotsos, Ioannis

doi:10.1007/s10561-010-9231-3

Cited by 13 publications

(7 citation statements)

References 38 publications

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“…PRP was prepared following the double-spinning method previously described. 17 A fixed volume (80 ml) of whole blood was initially centrifuged at 2,400 rotations per minute (rpm) for 10 min to separate PRP and platelet-poor plasma (PPP) from the red blood cell fraction. A second centrifugation cycle at 3,600 rpm for 15 min was performed to separate PRP from PPP.…”

Section: Prp Preparationsmentioning

confidence: 99%

Platelet‐rich plasma protects rotator cuff‐derived cells from the deleterious effects of triamcinolone acetonide

Muto

Kokubu

Mifune

et al. 2012

Journal Orthopaedic Research

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Triamcinolone acetonide (TA) injections are widely used to treat enthesopathy, but they may induce adverse effects such as tendon impairment and rupture. Platelet-rich plasma (PRP) is a blood fraction containing high platelet concentrations and various growth factors that play a role in tissue repair processes. The purpose of this study is to investigate whether TA has deleterious effects on human rotator cuff-derived cells, and if PRP can protect these cells from the effects of TA. Human rotator cuff-derived cells were cultured with and without TA and PRP, and the culture without any additive served as the control. Cell morphology was assessed at days 7 and 21. Cell viability was evaluated at days 1, 7, 14, and 21 by a water-soluble tetrazolium salt assay. Induction of apoptosis was measured by immunofluorescence staining and flow cytometry at day 7. Induction of cleaved caspase-3 was measured by immunofluorescence staining at day 7. The cells cultured with TA had a flattened and polygonal shape at day 7. The cells cultured with both TA and PRP were similar in appearance to control cells. Exposure to TA also significantly decreased cell viability, but cell viability did not decrease when PRP was added along with TA. The number of apoptotic cells increased with TA exposure, while addition of PRP prevented cell apoptosis. In conclusion, the deleterious effect of TA was prevented by PRP, which can be used as a protective agent for patients receiving local TA injections. Keywords: triamcinolone acetonide (TA); platelet-rich plasma (PRP); rotator cuff-derived cells; apoptosis; cell viability Enthesopathy caused by rotator cuff injury clinically impairs joint function. Rotator cuff disease represents a spectrum of pathological conditions ranging from tendinosis to full-thickness tears. Conservative therapy, including rest, physical therapy, non-steroidal anti-inflammatory medications, and steroid injections are the primary treatments for these conditions. 1,2The effectiveness of subacromial steroid injections for treatment of rotator cuff tendinosis has been equivocal. 3,4 Some clinical studies have demonstrated improvement in shoulder function and decrease in shoulder pain following steroid injection.3,5 However, other studies have shown that steroids afford no clinical benefits over those provided by injections of lidocaine alone 4 or physical therapy alone. 6 Triamcinolone acetonide (TA) injections are widely used for treatment of rotator cuff tendinosis, but they may cause tendon impairment and rupture; many cases of tendon rupture after TA injections have been reported. 7-11Despite previous studies on the deleterious histological and biomechanical changes in tendons after TA injections, 8,12 TA administration is still considered to be a conservative treatment option.Platelet-rich plasma (PRP) is an autologs concentration of platelets that contains many growth factors, including platelet-derived growth factor (PDGF), transforming growth factor b (TGF-b), fibroblastic growth factor (FGF), vascular endoth...

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Section: Prp Preparationsmentioning

confidence: 99%

Platelet‐rich plasma protects rotator cuff‐derived cells from the deleterious effects of triamcinolone acetonide

Muto

Kokubu

Mifune

et al. 2012

Journal Orthopaedic Research

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“…Sin embargo, no se conoce la respuesta celular a diferentes tiempos después a su activación. Diversos estudios han evaluado el uso del PRP en diferentes concentraciones que fluctúan entre 0,05 % y 100 % (8,34,35,37,44,45) y sus resultados son heterogéneos.…”

Section: Introductionunclassified

Evaluación del efecto del plasma rico en plaquetas en diferentes tiempos y concentraciones sobre la viabilidad de fibroblastos de ligamento periodontal y osteoblastos / Evaluation of Platelet-Rich Plasma Effect at different times and concentrations ...

et al. 2017

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Dabeiba Adriana García RobayoBacterióloga. magistra en Microbiología, doctora en Ciencias Biológicas, profesora asistente, investigadora. Centro de Investigaciones Odontológicas, Facultad de Odontología, Pontificia Universidad Javeriana. Bogotá, Colombia. Ximena Edilia Moreno SerranoOdontóloga, residente Posgrado Periodoncia, Pontificia Universidad Javeriana. Bogotá, Colombia. CÓMO CITAR ESTE ARTÍCULOAcosta AP, Gutiérrez SJ, Bedoya MA, García DA, Moreno XE. Evaluación del efecto del plasma rico en plaquetas en diferentes tiempos y concentraciones sobre la proliferación de fibroblastos de ligamento periodontal y osteoblastos.Univ Odontol. 2017 Ene-Jun; 36 (76) To evaluate proliferation and cell viability of periodontal ligament fibroblasts and osteoblasts stimulated with PRP in several concentrations and times after PRP activation. Methods: An in vitro study was carried out using periodontal ligament fibroblast and osteoblast cell cultures. PRP from venous blood of a healthy adult was prepared through centrifugation and activated with 10 % CaCl2. The effect on cell proliferation after application of 1 %, 3 %, and 5% PRP and plateletpoor plasma was evaluated at 0, 12, 24, 48, and 72 hours after activation through MTS. The control group consisted of culture that did not receive any treatment. Data were analyzed using Chi square, Fisher, and McNemar tests. Results: The cell viability assay showed statistically significant differences between the experimental and the control groups. Cell viability increased in cells treated with 5 % PRP 24 hours after activation (p=0.05). Conclusions: Fibroblast and osteoblast cell lines tended to be more viable 24 hours after activation with 5% PRP.

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“…Some promising strategies aimed at increasing osteoinductivity of allograft bone include co-delivery of stem cells [3–5], platelet rich plasma [6–8], and recombinant proteins [4, 9–11]. Platelet derived growth factor BB (PDGF-BB) [12] and bone morphogenic proteins (BMP) [13] are examples of recombinant proteins that have been co-mixed with allograft bone for the treatment of osseous defects.…”

Section: Introductionmentioning

confidence: 99%

Polyglutamate directed coupling of bioactive peptides for the delivery of osteoinductive signals on allograft bone

et al. 2013

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Allograft bone is commonly used as an alternative to autograft, however allograft lacks many osteoinductive factors present in autologous bone due to processing. In this study, we investigated a method to reconstitute allograft with osteoregenerative factors. Specifically, an osteoinductive peptide from collagen I, DGEA, was engineered to express a heptaglutamate (E7) domain, which binds the hydroxyapatite within bone mineral. Addition of E7 to DGEA resulted in 9× greater peptide loading on allograft, and significantly greater retention after a 5-day interval with extensive washing. When factoring together greater initial loading and retention, the E7 domain directed a 45-fold enhancement of peptide density on the allograft surface. Peptide-coated allograft was also implanted subcutaneously into rats and it was found that E7DGEA was retained in vivo for at least 3 months. Interestingly, E7DGEA peptides injected intravenously accumulated within bone tissue, implicating a potential role for E7 domains in drug delivery to bone. Finally, we determined that, as with DGEA, the E7 modification enhanced coupling of a bioactive BMP2-derived peptide on allograft. These results suggest that E7 domains are useful for coupling many types of bone-regenerative molecules to the surface of allograft to reintroduce osteoinductive signals and potentially advance allograft treatments.

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The effect of platelet-rich plasma (PRP) combined with a bone allograft on human periodontal ligament (PDL) cells

Cited by 13 publications

References 38 publications

Platelet‐rich plasma protects rotator cuff‐derived cells from the deleterious effects of triamcinolone acetonide

Platelet‐rich plasma protects rotator cuff‐derived cells from the deleterious effects of triamcinolone acetonide

Evaluación del efecto del plasma rico en plaquetas en diferentes tiempos y concentraciones sobre la viabilidad de fibroblastos de ligamento periodontal y osteoblastos / Evaluation of Platelet-Rich Plasma Effect at different times and concentrations ...

Polyglutamate directed coupling of bioactive peptides for the delivery of osteoinductive signals on allograft bone

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