The effect of platelet concentrate storage temperature on adenine nucleotide metabolism

Filip, Donald J.; Eckstein, John D.; Sibley, Carol A.

doi:10.1182/blood.v45.6.749.749

Cited by 24 publications

(9 citation statements)

References 18 publications

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“…'9 Platelets were first separated from platelet concentrates as described above and were suspended in buffered saline to a platelet concentration of 1. 6 x lo9 platelets/ml. This suspension was mixed with an equal volume of buffered saline containing M CaCI, to give a final calcium concentration of 5 x M and a final platelet concentration of 8 x 10" platelets/ml.…”

Section: Relecise Of Cnlciummentioning

confidence: 99%

Increased Thrombin Sensitivity of Human Platelets After Storage at 4 C

et al. 1979

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In vitro function of fresh platelets and platelets stored for 24 hours at 4 C and 22 C was studied using the release of 14C‐5‐hydroxytryptamine (5‐HT), adenine nucleotides and calcium following stimulation with thrombin. Storage of platelets did not affect 14C‐5‐HT uptake capability, adenine nucleotide content or adenine nucleotide release. Platelets stored in 4 C varied from fresh platelets and 22 C‐stored platelets with respect to 5‐HT and calcium release. For measurement of 5‐HT release, different thrombin‐to‐platelet concentrations were used. At thrombin‐to‐platelet concentrations of 0.013 to 0.02 U/108 platelets, 4 C‐stored platelets released more 5‐HT than did fresh platelets. Differences between 4 C‐stored platelets and 22 C stored platelets were observed over a wider range of thrombin‐to‐plate‐lets concentrations. The 4 C‐stored platelets released significantly greater amounts of 5‐HT at thrombin‐to‐platelet concentrations of 0.008‐0.02 L/ml. Calcium release, measured with the calcium electrode, was studied at high (1.0 L/ml) and low (0.06 U/ml) thrombin concentrations and at a platelet concentration of 8 × 108 platelets/ml. At the high thrombin concentration, the amount of calcium released was the same for all platelets studied. At the low thrombin concentration, platelets stored at 4 C released a greater amount of calcium than did either fresh platelets or platelets stored at 22 C. The results obtained with different thrombin‐to‐platelet concentrations suggest that there is only a narrow range of thrombin concentrations per platelet at which the subtle differences between platelet populations may be detected. The range may reflect the threshold concentration of thrombin that is required to initiate a release reaction. The apparently lower thrombin threshold required by 4 C platelets to undergo a release reaction may be a factor contributing to their reduced circulation and superior hemostatic effectiveness.

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Section: Relecise Of Cnlciummentioning

confidence: 99%

Increased Thrombin Sensitivity of Human Platelets After Storage at 4 C

et al. 1979

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“…KIM and BALDINI (1972) found that after 48 hours of storage 78 % of the initially measured ATP concentration remained compared to 76 % in this study. FILIP et al (1975) measured a diminution of 27 % after 72 hours. We found a decrease of 24 % after 48 hours and of 25 % after 96 hours.…”

Section: Discussionmentioning

confidence: 97%

“…Since most authors agree that nucleotides are better preseved at 4 "C (ODAKE and AMBRUS, 1969;FILIP et al, 1975;RAO and MURPHY, 1982) we chose 4 "C as storage temperature in our study. The results of our study were markedly different from results of investigations where platelets were stored at 22 "C (ORENGO et al, 1980;RAo et al, 1981).…”

Section: Discussionmentioning

confidence: 99%

“…While there are no references on changes in the nucleotide concentration of dog platelets, many authors investigated the storage ability of human platelets by measuring platelet nucleotide concentration (KOTELBA-WITKOWSKA et al, 1972;FILIP et al, 1975;BEUTLER and KUHL, 1980;RAo and MURPHY, 1982). Most authors monitored the changes for 1 to 3 days.…”

Section: Discussionmentioning

confidence: 99%

“…cattle, cats, guinea pigs (DODDS, 1978;GOETZ et al, 1978;DONNER and HOUSKOVA, 1972;FLODGAARD et al, 1986) have been analyzed. Most authors examined platelets from freshly drawn blood or after storage for up to 3 days (KOTELBA-WITKOWSKA et al., 1972;FILIP et al, 1975;BEUTLER and KUHL, 1980;RAO and MURPHY, 1982).…”

Section: Introductionmentioning

confidence: 99%

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The Influence of Storage Time on the Nucleotide Concentration of Canine Platelets

Gäbler

Nolte

Scheiner-Bobis

et al. 1993

Journal of Veterinary Medicine Series A

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Summary Nucleotide concentrations of canine platelets during storage for 50 days were determined to find out the variation in the concentration of various nucleotides in canine platelets during storage at + 4 °C for 50 days. Platelets of 3 dogs were stored in whole blood in PVC bags containing CPDA‐1 stabilizer. The bags were kept at 4 °C for 50 days. Changes in the energy metabolism of the platelets during storage were determined by successive measurements of the following nucleotides: adenosine‐diphosphate (ADP), adenosine‐tri‐phosphate (ATP) and guanosine‐tri‐phosphate (GTP). In preparation for the measurement of their nucleotide content, the platelets were isolated from the blood at regular intervals and their membranes destroyed by deep freezing, thawing, double sonification in ice and protein precipitation with perchloric acid. The concentration of nucleotides within the platelets was determined by “High Pressure Liquid Chromatography” (HPLC). ADP, ATP and GTP were identified in the peak areas of the HPLC using 3 different techniques: comparison of retention time of standard nucleotide solutions as control samples, enzyme tests and thin layer chromatography. Adenosine‐mono‐phosphate (AMP), adenosine, hypoxanthine and inosine mono 5‘‐phosphate (IMP) were not found in dog platelets. Platelet ATP concentration decreased significantly during 50 days of storage (p < 0.001), and was reduced to 24% of the concentration measured after 1 hour of storage on day 50. ADP concentration also dropped significantly during 50 days of storage (p < 0.001). On day 50 the average ADP concentration was reduced to about 34% of the concentration measured after 1 hour of storage. The greatest reduction was observed in GTP concentration (p < 0.001): after 50 days the GTP concentration was only 5% of the concentration measured after 1 hour of storage. In freshly drawn blood samples, the average ATP/ADP‐ratio was 1.7 and the average ATP/GTP‐ratio was 5.5. In all 3 dogs the ATP/ADP‐ratios reached their lowest values between the 36th and 43rd day of storage, at which time the ratios were clearly below 1. After this time the ATP/ADP‐ratios increased and were bigger than 1 after 50 days (an average of 75% of the values measured after 1 hour of storage). During storage the GTP concentration decreased much faster than the ATP concentration. On day 50 the ATP/GTP‐ratio was 33.3. Our results indicate in concordance with the literature, that below a certain energy level, the platelets lose their functions and concentrate on surviving by saving energy for their metabolism.

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Komplikationen und supportive Therapie der Leukämien und malignen Non-Hodgkin-Lymphome

Brittinger¹,

König²

1978

Blut Und Blutkrankheiten

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The effect of platelet concentrate storage temperature on adenine nucleotide metabolism

Cited by 24 publications

References 18 publications

Increased Thrombin Sensitivity of Human Platelets After Storage at 4 C

Increased Thrombin Sensitivity of Human Platelets After Storage at 4 C

The Influence of Storage Time on the Nucleotide Concentration of Canine Platelets

Komplikationen und supportive Therapie der Leukämien und malignen Non-Hodgkin-Lymphome

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