The effect of plasmin on the facility of outflow in cynomolgus monkeys

Perkins, E. S.; Saiduzzafar, H.

doi:10.1016/s0014-4835(69)80003-5

Cited by 10 publications

(2 citation statements)

References 6 publications

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“…Earlier investigations have described the presumed roles of plasmino gen activator and plasmin in maintenance of microvascular and aqueous humor flows [Pandolfi and Astrup, 1967 ;Perkins and Saiduzzafar, 1969], and in prevention of excessive induration and fibrosis at sites of tissue injury [Brakman, 1967;Pandolfi et al, 1962;Kwaan and Astrup, 1963]. Further evidence suggests that vascular, trabecular meshwork and, perhaps, corneal endothelium are prime sources of anterior segment plas minogen activator [Pandolfi and Astrup, 1967;Fehrenbacher et al, 1979].…”

Section: Discussionmentioning

confidence: 99%

Evidence of Impaired Anterior Segment Fibrinolytic Activity in Chronic Uveitis

et al. 1982

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Purified homologous fibrinogen (2.4–9.4 mg) was injected intracamerally in cat eyes at approximate six months intervals for up to 30 months in order to assess, in vivo, the long-term impact of chronic uveitis on anterior segment fibrinolytic capability. Normal and acutely inflamed eyes responded with slow clotting, small clot formation and rapid lysis (2–3 days). Chronically inflamed eyes showed rapid clotting, larger clots and much slower lysis, with BCG-induced uveitis much slower than endotoxin-induced uveitis. Suppression of plasminogen activator levels within aqueous humor of chronically inflamed eyes was indicated, using modified fibrin plate methods. The normal balance between fibrinolysis and fibrinogenesis within tissues bounding the anterior chamber is apparently tilted in favor of the latter function when inflammation is prolonged.

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Section: Discussionmentioning

confidence: 99%

Evidence of Impaired Anterior Segment Fibrinolytic Activity in Chronic Uveitis

et al. 1982

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“…Furthermore, it was reported that the gene expressions of some fibrinolytic proteins in cultured TM tissues obtained from patients with POAG were different as compared to those in cultured normal TM tissues [8], and it was suggested that the fibrinolytic system may be associated with regulation of IOP. In fact, Perkinse et al [9] reported that injection of plasmin into the anterior chambers of eyes in living cynomolgus monkeys caused a substantial and statistically significant increase in the coefficient of facility of aqueous outflow.…”

Section: Introductionmentioning

confidence: 99%

Controllable Urokinase Gene Expression in Trabecular Meshwork Cells

Tsuda¹

2014

J Clin Exp Ophthalmol

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Purpose: Glaucoma is a type of progressive optic neuropathy that finally leads to blindness related to elevated intraocular pressure (IOP). Accumulation of extracellular matrix in trabecular meshwork (TM) and juxta-canalicular connective tissues, which form an aqueous outflow pathway, may be a major cause of increased IOP, thus the fibrinolytic system may be associated with regulation of IOP. We examined the possibility of controllable urokinase plasminogen activator (uPA) gene transfer into TM cells. Methods: TM cells were freshly isolated from porcine eyes and human TM cells obtained during trabeculectomy procedures and cultured. Total RNA was extracted from human TM cells and reverse transcribed into cDNA. A reverse-transcribed polymerase chain reaction (RT-PCR) method was then performed to detect the gene expression of uPA. The cDNA of human uPA was sub-cloned into an expression vector (pEYFP-N1 vector) and controllable expression vector (TRE-Tight vector), then each vector was independently transfected into cultured porcine TM cells. Doxycycline was added to the culture medium to activate the pTet-ON and TRE-Tight vectors. Finally, the expression of uPA was examined using an enzyme linked immunosorbent assay (ELISA). Results: ELISA findings revealed the expression of uPA (3.5 ng/ml) in medium from cultured porcine TM cells that had been transfected with the human uPA gene using the pEYFP-N1 vector. Doxycycline induced human uPA in the pTet-On and TRE-Tight vectors with human uPA gene co-transfected TM cells in a dose-dependent manner. Conclusions: Controllable gene transfer of uPA, which may degrade the extracellular matrix in juxta-canalicular connective tissue, into TM cells was achieved using a Tet-On system. The present method may be useful as a novel therapy for glaucoma.

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Coagulation Factor VIII

Pandolfi¹

1976

Arch Ophthalmol

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The effect of plasmin on the facility of outflow in cynomolgus monkeys

Cited by 10 publications

References 6 publications

Evidence of Impaired Anterior Segment Fibrinolytic Activity in Chronic Uveitis

Evidence of Impaired Anterior Segment Fibrinolytic Activity in Chronic Uveitis

Controllable Urokinase Gene Expression in Trabecular Meshwork Cells

Coagulation Factor VIII

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