The effect of plant growth retardants on the biosynthesis of diterpenes by Gibberella fujikuroi

Cross, B. E.; Myers, Peter L.

doi:10.1016/s0031-9422(00)85798-2

Cited by 72 publications

(22 citation statements)

References 13 publications

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“…ABA production by the fungus and the inhibition of ABA by decylimidazole at 48 h is shown in Figure 1. Ancymidol was the second most active inhibitor of ABA biosynthesis. Coolbaugh et al (6) reported that ancymidol is a highly specific inhibitor of three oxidative reactions in the GA biosynthetic pathway in higher plants but that it does not inhibit GA (7) and inhibits the conversion of acetate and mevalonate to sterols (8,9). AMO 1618 inhibited ABA biosynthesis in C. rosicola.…”

Section: Resultsmentioning

confidence: 99%

Inhibition of Abscisic Acid Biosynthesis in Cercospora rosicola by Inhibitors of Gibberellin Biosynthesis and Plant Growth Retardants

Norman

Poling²,

Maier³

et al. 1983

Plant Physiol.

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The fungus Cercospora rosicola produces abscisic acid (ABA) as a secondary metabolite. We developed a convenient system using this fungus to determine the effects of compounds on the biosynthesis of ABA. Inasmuch as ABA and the gibberellins (GAs) both arise via the isoprenoid pathway, it was of interest to determine if inhibitors of GA biosynthesis affect ABA biosynthesis. AU five putative inhibitors of GA biosynthesis tested inhibited ABA biosynthesis. Several plant growth retardants with poorly understood actions in plants were also tested; of these, six inhibited ABA biosynthesis to varying degrees and two had no effect. Effects of plant growth retardants on various branches of the isoprenoid biosynthetic pathway may help to explain some of the diverse and unexpected results reported for these compounds. Knowledge that certain inhibitors of GA biosynthesis also have the ability to inhibit ABA biosynthesis in C. rosicola indicates the need for further studies in plants on the mode of action of these compounds.

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Section: Resultsmentioning

confidence: 99%

Inhibition of Abscisic Acid Biosynthesis in Cercospora rosicola by Inhibitors of Gibberellin Biosynthesis and Plant Growth Retardants

Norman

Poling²,

Maier³

et al. 1983

Plant Physiol.

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“…Inhibition of kaurene formation from mevalonate by AMO was also found in preparations from pea seeds (71) and fruits (72); synthesis of squalene and phytoene was not affected (72). The inhibition of kaurene and GAa production in Gibberella by AMO and CCC was confirmed by chemical a Some investigators, especially Brian (4) and Cross (5,110), are persistently playing down the validity of the earlier, bioassay-based studies on the action of the retardants.…”

Section: Inhibitory Effectb Onmentioning

confidence: 86%

Gibberellins: Structure and Metabolism

Lang¹

1970

Annu. Rev. Plant. Physiol.

245

118

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“…Samson) seeds were germinated in potting compost and grown in a glasshouse at 18 to 24 C. Immediately before an experiment, when the seedlings were 2 to 3 cm in length (usually 3-4 weeks after germination), they were removed, thoroughly washed, and the roots were excised. A number of rootless seedlings (totaling about 300 mg in weight) were placed in a solution of 5 mg/ml, was added to one set of Petri dishes, and the pH was readjusted to 6.5. Final volume in all treatments was 2 ml.…”

Section: Methodsmentioning

confidence: 99%

“…Although retardant effects have been explored in such diverse systems as fungi (5,8), higher plants (4,7), insects (3,18), and mammals (9,11), no one set of results satisfactorily accounts for the great diversity of organismic responses.…”

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confidence: 99%

Inhibition of Sterol Biosynthesis by 2-Isopropyl-4-dimethylamino-5-methylphenyl-1-piperidine Carboxylate Methyl Chloride in Tobacco and Rat Liver Preparations

Douglas

Paleg

1972

Plant Physiol.

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(3,18), and mammals (9, 11), no one set of results satisfactorily accounts for the great diversity of organismic responses.When a group of compounds produces different results in different systems, it is important to assess carefully whether the effects recorded with one species are in any way pertinent to those recorded for other species. This paper presents evidence which strongly supports the contention that at least one retardant effect observed in cell-free rat liver systems has direct relevance to the effects of the retardant on higher plants. It is also the first report of the inhibition of sterol biosynthesis in plants by a growth retardant. MATERIALS AND METHODSPlant Tissue. Nicotiana tabacum (cv. Samson) seeds were germinated in potting compost and grown in a glasshouse at 18 to 24 C. Immediately before an experiment, when the seedlings were 2 to 3 cm in length (usually 3-4 weeks after germination), they were removed, thoroughly washed, and the roots were excised. A number of rootless seedlings (totaling about 300 mg in weight) were placed in a solution of 5 yC DL-2-'4C-mevalonic acid lactone in 11 mm phosphate buffer, pH 6.5, in 5-ml Petri dishes. Amo 1618,1 at a final concentration of 1 'Abbreviations: Amo 1618: 2-isopropyl-4-dimethylamino-5-methylphenyl-l-piperidine carboxylate methyl chloride, 90%; CCC: 2-chloroethyl-trimethyl ammonium chloride; SK & F 7997: tris(2-diethylaminoethyl)phosphate hydrochloride. mg/ml, was added to one set of Petri dishes, and the pH was readjusted to 6.5. Final volume in all treatments was 2 ml. The seedlings were placed around the edge of the dishes so that only the cut stems were immersed in the solution. The Petri dishes containing the seedlings were placed in a closed, clear plastic container and left under constant illumination (230 ft-c)for 24 hr at a constant temperature of 18 C.Extraction. After incubation, the seedlings were removed with forceps and homogenized in three successive 5-ml portions of ethanol-benzene (4:1), with an Ultra-turrax blender. The combined extracts were filtered through Whatman No. 1 filter paper and reduced to dryness in vacuo in a rotary evaporator.The residue was taken up in 5 ml of water, and the lipids were extracted with diethyl ether (4 X 5 ml). The combined ether extracts were washed with water (2 X 5 ml), and the ether fraction was evaporated in vacuo. The lipid residue was redissolved in 20 ml of hexane and extracted with 80% (v/v) methanol (3 X 20 ml) to remove acidic and polar lipids. The hexane was then removed in vacuo, and the residue was saponified under reflux with 5% (w/v) KOH in 85% (v/v) methanol (5 ml) for 60 min. All nonsaponifiable lipid material was then extracted with diethyl ether (3 X 10 ml), and the combined extracts were washed with water (5 ml). The ether was removed in vacuo, and the lipid residue was dissolved in benzene.Thin Layer Chromatography. Nonsaponifiable lipid fractions were spotted on thin layer plates in an atmosphere of dry nitrogen. An aliquot of the benzene solution was spotted on a Silica G...

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The effect of plant growth retardants on the biosynthesis of diterpenes by Gibberella fujikuroi

Cited by 72 publications

References 13 publications

Inhibition of Abscisic Acid Biosynthesis in Cercospora rosicola by Inhibitors of Gibberellin Biosynthesis and Plant Growth Retardants

Inhibition of Abscisic Acid Biosynthesis in Cercospora rosicola by Inhibitors of Gibberellin Biosynthesis and Plant Growth Retardants

Gibberellins: Structure and Metabolism

Inhibition of Sterol Biosynthesis by 2-Isopropyl-4-dimethylamino-5-methylphenyl-1-piperidine Carboxylate Methyl Chloride in Tobacco and Rat Liver Preparations

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