The Effect of Pithing and of Nerve Stimulation on the Depletion of Noradrenaline by Reserpine in the Rat Anococcygeus Muscle and Vas Deferens

Gillespie, John; McGrath, J.C.

doi:10.1111/j.1476-5381.1974.tb09727.x

Cited by 44 publications

(44 citation statements)

References 22 publications

(25 reference statements)

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“…The effect mine. Using reserpine alone, Sj6strand & Swedin one was identical with (1968) and Gillespie & McGrath (1974) showed that o L it was more difficult to deplete noradrenaline in the vas deferens than in the heart though doses of reserpine higher than 1.0 mg/kg were usually effective in both tissues. Sjostrand & Swedin (1968) thought that this could be due to the properties of the short adrenergic neurones which supply the vas deferens, or less likely to poor circulation to the organ.…”

Section: Dicussionmentioning

confidence: 85%

“…Sjostrand & Swedin (1968) thought that this could be due to the properties of the short adrenergic neurones which supply the vas deferens, or less likely to poor circulation to the organ. Gillespie & McGrath (1974) showed that it was more likely to be due to much less frequent stimulation of the vas than the heart in the intact reserpine-treated animals, since stimulation of the sympathetic nerves to the vas, after reserpine treatment increased the depleting effect of the drug. Therefore, to ensure that the maximum possible depletion of the noradrenaline in the vas was obtained in these experiments, a high dose of reserpine was used and the extent of the depletion of noradrenaline in the heart was measured for comparison.…”

Section: Dicussionmentioning

confidence: 99%

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The Effects of Release and Depletion of Endogenous Noradrenaline on the Transmission of Impulses in the Mouse Vas Deferens

Marshall

Nasmyth

Shepperson

1978

British J Pharmacology

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1 The effects of endogenous noradrenaline released by tyramine and the influence of depletion of the tissue noradrenaline with reserpine and/or oa-methyl-p-tyrosine on the twitch responses of the field-stimulated mouse vas deferens have been studied.2 Tyramine (10-40 gM) inhibited the twitch responses to field stimulation and failed to produce a contraction. The inhibition decreased as the rate of stimulation increased.3 The inhibition produced by tyramine was antagonized by cocaine (10 PM) and by yohimbine (10 nM), which indicated that it was produced by released noradrenaline acting on presynaptic a-adrenoceptors. 4 Depletion of the tissue noradrenaline by 39% by blockade of the synthesis of noradrenaline with a-methyl-p-tyrosine, was without effect on the twitch response but it reduced the inhibitory effect of tyramine. 5 Depletion of the tissue noradrenaline by 96.5% with reserpine alone and by 99.4%, with a combination of reserpine and ax-methyl-p-tyrosine, reduced the twitch responses by approximately 66% and virtually abolished the inhibition produced by tyramine. It also increased the rate of decline of the responses when the tissue was continuously stimulated. The remaining twitch was not antagonized by phenoxybenzamine (15 gM).6 Residual twitches were bigger in tissues depleted by 99.4% than in those depleted by only 96.5%. This difference was eliminated in the presence of yohimbine (128 nM). 7 It is concluded that inhibition of the twitch responses by tyramine is produced by stimulation of presynaptic a-adrenoceptors and that the twitch response is associated with stimulation of the sympathetic neurone, but that it is not mediated by postsynaptic a-adrenoceptors.

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Section: Dicussionmentioning

confidence: 85%

Section: Dicussionmentioning

confidence: 99%

The Effects of Release and Depletion of Endogenous Noradrenaline on the Transmission of Impulses in the Mouse Vas Deferens

Marshall

Nasmyth

Shepperson

1978

British J Pharmacology

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“…This schedule reduces the noradrenaline content of the rat vas deferens by over 98% (Gillespie & McGrath, 1974).…”

Section: Methodsmentioning

confidence: 99%

“…Following reserpine, no 'twitch' is present and the response is monophasic. Previously the effect of reserpine could not be clearly interpreted since it was thought that the 'twitch' was 'nonadrenergic' (Ambache & Zar, 1971), yet it was reduced by reserpine (Gillespie & McGrath, 1974). …”

Section: Trains Of Stimulimentioning

confidence: 99%

Separation of adrenergic and non‐adrenergic contractions to field stimulation in the rat vas deferens

Brown

Docherty

French

et al. 1983

British J Pharmacology

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126

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“…The muscle has a dense adrenergic motor innervation, but there is no evidence of cholinergic nerves; it does, however, possess a powerful inhibitory innervation mediated by an unknown transmitter (Gillespie, 1972;Gillespie & McGrath, 1972).…”

Section: Introductionmentioning

confidence: 99%

The Response of the Cat Anococcygeus Muscle to Nerve or Drug Stimulation and a Comparison With the Rat Anococcygeus

Gillespie

McGrath

1974

British J Pharmacology

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1The cat anococcygeus muscle is shown to possess a dual innervation similar to the rat anococcygeus, with a motor adrenergic innervation and an inhibitory innervation whose transmitter is unknown. The pharmacological properties of the cat muscle were invesjigated and compared with those of the rat muscle.2 The cat muscle contracts to noradrenaline, 5-hydroxytryptamine, tyramine, amphetamine, guanethidine, cocaine and lysergic acid diethylamide (LSD). The effects of noradrenaline and 5-hydroxytryptamine are blocked by phentolamine and methysergide respectively. 3 The cat anococcygeus is relaxed by acetylcholine, carbachol, isoprenaline, ATP, prostaglandins E1, E2 and F2a, and vasopressin, all of which contract the rat muscle. The effects of acetylcholine and carbachol are blocked by atropine and those of isoprenaline by propranolol. 4 Field stimulation produces contraction of the cat anococcygeus, which is blocked by phentolamine and guanethidine but unaffected by hexamethonium, atropine or neostigmine.5 In the presence of guanethidine (10-5 M), the tone of the muscle is raised and field stimulation produces relaxation of the muscle. These inhibitory responses are unaffected by phentolamine, hexamethonium, atropine or neostigmine. 6 Neostigmine potentiates the effects of acetylcholine, but not of carbachol in relaxing the cat anococcygeus and in contracting the rat anococcygeus, but has no effect on either motor or inhibitory responses to field stimulation. 7 Cold storage for up to eight days had little effect on either the motor response to noradrenaline or the motor or inhibitory response to field stimulation of the cat anococcygeus. Beyond eight days, the response to field stimulation diminishes more rapidly than the response to noradrenaline.

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The Effect of Pithing and of Nerve Stimulation on the Depletion of Noradrenaline by Reserpine in the Rat Anococcygeus Muscle and Vas Deferens

Cited by 44 publications

References 22 publications

The Effects of Release and Depletion of Endogenous Noradrenaline on the Transmission of Impulses in the Mouse Vas Deferens

The Effects of Release and Depletion of Endogenous Noradrenaline on the Transmission of Impulses in the Mouse Vas Deferens

Separation of adrenergic and non‐adrenergic contractions to field stimulation in the rat vas deferens

The Response of the Cat Anococcygeus Muscle to Nerve or Drug Stimulation and a Comparison With the Rat Anococcygeus

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