The effect of pinealectomy and autonomic denervation on crypt cell proliferation in the rat small intestine

Callaghan, B. D.

doi:10.1111/j.1600-079x.1991.tb00813.x

Cited by 21 publications

(31 citation statements)

References 27 publications

(7 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(c) There were no differences between groups in average apoptotic ratio in the duodenum, jejunum, or ileum. (d) There were no differences between groups in average total villus cell number in the duodenum, jejunum, or ileum Several groups demonstrate that proliferation increases one week post-PNS denervation in the jejunum 4,5 and the ileum in rats. 4 However, others suggest that there is no difference in the ileum compared to sham controls at 6 or 10 days postdenervation.…”

Section: Discussionmentioning

confidence: 99%

“…(d) There were no differences between groups in average total villus cell number in the duodenum, jejunum, or ileum Several groups demonstrate that proliferation increases one week post-PNS denervation in the jejunum 4,5 and the ileum in rats. 4 However, others suggest that there is no difference in the ileum compared to sham controls at 6 or 10 days postdenervation. 2 Despite these discrepancies in proliferation at early time points, human jejunal biopsies post-PNS denervation have suggested that there is no change in intestinal proliferation three and six weeks post-surgery.…”

Section: Discussionmentioning

confidence: 99%

“…2,3 The early decrease in proliferation may be due to a concomitant decrease in food intake, which is known to decrease intestinal epithelial cell proliferation independent of an ANS influence. 8 At later time points post-PNS denervation, proliferation has been shown to be increased 4,5 or equal to that of control animals, 2,9 perhaps demonstrating a recovery over time. 2 Thus, these changes may be indirectly driven by changes in food intake after PNS denervation, rather than a direct influence of the loss of the PNS.…”

Section: Introductionmentioning

confidence: 99%

“…6,7 However, a more focused surgical denervation targeting the celiacomesenteric nerves that innervate the intestine results in no change in proliferation along most of the proximal to distal axis of the intestine. [2][3][4]6 Thus, we propose that PNS or SNS denervation does not have a long-term effect on intestinal epithelial regeneration when you control for postsurgical changes in food intake and use minimally invasive surgical techniques. A recovery of function after long-term ANS denervation is seen in processes related to intestinal function, including motility, 10 food intake 11,12 and blood flow.…”

Section: Introductionmentioning

confidence: 99%

“…A focus of prior research has been on the ANS control of intestinal epithelial cell turnover, as it is among the most rapidly regenerating tissues of the body. 1 Ablation of either branch of the ANS, the parasympathetic (PNS) or sympathetic (SNS), alters intestinal epithelial cell proliferation as indicated by a change in the mitotic index [2][3][4][5][6][7] and length of the cell cycle. 6 These data, though, are inconsistent across studies.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Long-term effect of parasympathetic or sympathetic denervation on intestinal epithelial cell proliferation and apoptosis

Davis

Washington

Yaniz

et al. 2017

Exp Biol Med (Maywood)

View full text Add to dashboard Cite

This study investigates the long-term effect of autonomic denervation on intestinal epithelial cell turnover, as measured by proliferation, apoptosis, and total cell number. Although previous research has established that autonomic denervation can alter intestinal epithelial turnover under short-term conditions, here we establish for the first time that these changes do not persist long-term when you control for surgical-induced changes in food intake and use targeted denervation procedures. These findings add to the base of knowledge on autonomic control of tissue turnover, highlight the ability of the intestinal epithelium to recover after autonomic injury and reveal possible implications of the use of ANS denervation for disease treatment in humans. AbstractIntestinal epithelial tissue is constantly regenerated as a means to maintain proper tissue function. Previous studies have demonstrated that denervation of the parasympathetic or sympathetic nervous system to the intestine alters this process. However, results are inconsistent between studies, showing both increases and decreases in proliferation after denervation of the parasympathetic or sympathetic. The effect appears to correlate with (1) the timing post-denervation, (2) denervation-induced changes in food intake, (3) the denervation technique used, and (4) which intestinal segment is investigated. Thus, we proposed that parasympathetic or sympathetic denervation does not have an effect on intestinal epithelial regeneration when you (1) evaluate denervation after long-term denervation, (2) control for post-surgical changes in food intake, (3) use minimally invasive surgical techniques and (4) include a segmental analysis. To test this, adult male Sprague Dawley rats underwent parasympathetic denervation via subdiaphragmatic vagotomy, sympathetic denervation via celiacomesenteric ganglionectomy, a parasympathetic denervation sham surgery, or a sympathetic denervation sham surgery. Sham surgery ad libitum-fed groups and sham surgery pair-fed groups were used to control for surgically induced changes in food intake. Three weeks post-surgery, animals were sacrificed and tissue from the duodenum, jejunum, and ileum was excised and immunohistochemically processed to visualize indicators of proliferation (bromodeoxyuridine-positive cells) and apoptosis (caspase-3-positive cells). Results showed no differences between groups in proliferation, apoptosis, or total cell number in any intestinal segment. These results suggest that parasympathetic or sympathetic denervation does not have a significant long-term effect on intestinal epithelial turnover. Thus, intestinal epithelial regeneration is able to recover after autonomic nervous system injury.

show abstract

Section: Discussionmentioning

confidence: 99%