and Pirovano, G. 1984. Effect of benzyladenine on development of derooted watermelon seedlings. -Physiol. Plant. 62: 615-620.We have examined the effects of benzyladenine (BA) on derooted watermelon (CitruUus vuigaris Schrad., cv. Fairfax) seediings with special attention to the cotyledons. The growth regulator was supplied either as a droplet of solution between the cotyledons (application from above) or through the hypocotyl stump (application from below). Application of BA from above stimulates several developmental parameters of cotyledons [growth, Iipid breakdown, chlorophyll and earotenoid accumulation, hydroxypyruvate reductase (EC 1.1.1.81.) activity]. The stimulation is much smaller with application from below. Also the distribution of labelled BA changes according to the method of application. The bulk of the label remains in the eotyledons when BA is supplied from above and in the hypocotyl when it is supplied from below. The absolute amount of radioactivity found in the cotyledons after 24 h of treatment is approximately the same in both cases. This seems to indicate that the different effects of the two treatments depend on concentration ratios of BA (or metabolic derivates) between hypocotyl and cotyledons rather than on absolute growth regulator levels.Additional key words -Cotyledons, cytokinin transport, hypocotyl,
G. P. Longo (reprint requests) et a