The effect of phototherapy on sister chromatid exchange with different light density in newborn hyperbilirubinemia

Abstract

Aim: Concerns of possible genotoxic effects of hyperbilirubinemia and phototherapy were raised from experimental and observational studies in neonates. The purpose of this study was to assess the effect of hyperbilirubinemia and phototherapy with three different methods on DNA damage by investigating sister chromatid exchange frequency. Material and Methods: Patients whose gestational ages were >37 weeks and bilirubin levels above phototherapy limits were enrolled into three groups and each group was planned to receive 25 babies. Group 1 received enhanced light-emitting diode phototherapy, group 2 had light-emitting diode phototherapy, and group 3 received conventional phototherapy. Infants with hyperbilirubinemia but did not require phototherapy comprised the control group, which was subdivided into two groups regarding bilirubin levels (<or>10mg/dL). Blood samples were collected before and after phototherapy for sister chromatid exchange frequency and samples were examined by a biologist who was blinded to the study groups. Results: The mean pre-treatment sister chromatid exchange frequency was 1.41±0.34/cell, post-treatment 2.65±0.68/cell, and 1.61±0.61/ cell for the control group (p<0.05). A statistically significant increase in sister chromatid exchange frequency after phototherapy was observed in all three intervention groups (p values: 0.01, 0.01, and 0.008, respectively). When the treatment groups were compared with each other in terms of irradiance, no significant difference was found (p=0.08). Conclusions: Phototherapy causes an increase in the frequency of sister chromatid exchange regardless of the irradiance. Phototherapy could have some genotoxic adverse effects on chromosomes; however, further investigations are warranted to enlighten as to whether these effects are permanent or clinically important.

Challenges of phototherapy for neonatal hyperbilirubinemia (Review)

“…It has also been suggested that neonatal phototherapy may increase the risk of hemangioma in infants, which may be associated with oxidative stress caused by phototherapy, which can damage vascular endothelial cells and stimulate the formation of new blood vessels ( 111 ). The increased risk of cancer in children treated with phototherapy may be associated with hyperbilirubinemia per se , phototherapy or a combination of the two ( 112 ).…”

Olgu sunumu: moleküler düzeyde tanısı konulmuş olan ilk Türk Rotor sendromlu aile

“…Subsequently, it is reabsorbed by OATP1B1 and OATP1B3 proteins in hepatocytes. Because OATP1B1 and OATP1B3 proteins are abnormal in RS, bilirubin reuptake and excretion by the liver is less efficient, and this causes accumulation of bilirubin in the blood and urine; this is reflected in the phenotype as jaundice and dark colored urine ( 4 – 7 ).…”

External Factors that Direct the Biological Clock and Role in Treatment of Disease

“…For example, light is used in modern medicine in the treatment of many diseases [42][43][44]. According to a study by Tuzun and Cesare different color fluorescences can be obtained when examining various diseases with a Wood light (black light), which is a fluorescent lamp that emits a purple light.…”