The Effect of Phototherapy on Cancer Predisposition Genes of Diabetic and Normal Human Skin Fibroblasts

Chotikasemsri, Pongsathorn; Tangtrakulwanich, Boonsin; Sangkhathat, Surasak

doi:10.1155/2017/7604861

Cited by 4 publications

(3 citation statements)

References 5 publications

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“…Interestingly, this sharp reduction in retinal PDE11A4 expression coincides with the approximate period when mice open their eyes for the first time (P10-13). This correlation is particularly interesting given a report showing that light exposure can regulate PDE11A4 expression in vitro [32]. In contrast, we observed a gradual age-related increase in PDE11A4 expression in the anterior segment (Figure 2B), which would reduce cAMP and cGMP.…”

Section: Pde11a4 Expression In the Eye Changes With Age In A Segment-...supporting

confidence: 63%

“…Although the presence of PDE11A protein has not yet been reported in the eye, its presence has been implied by human single-cell RNA sequencing studies [24][25][26][27][28]. Further, myopic development is associated with inadequate exposure to light [13,29,30], photo-transduction is highly dependent on hydrolysis of the PDE11A substrate cGMP [31], and specific wave lengths of light that upregulate PDE11A expression in fibroblast cells [32] demonstrate promise as potential anti-myopic agents [29]. Of particular interest to the fact that PDE11A-Y727C is both protective against myopia and enhancing to sleep quality, myopia has been associated with reduced sleep quality [33,34] and a dysregulated retinal circadian clock [35,36].…”

Section: Introductionmentioning

confidence: 99%

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The Sleep Quality- and Myopia-Linked PDE11A-Y727C Variant Impacts Neural Physiology by Reducing Catalytic Activity and Altering Subcellular Compartmentalization of the Enzyme

Sbornova,

van der Sande,

Milosavljevic

et al. 2023

Cells

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Recently, a Y727C variant in the dual-specific 3′,5′-cyclic nucleotide phosphodiesterase 11A (PDE11A-Y727C) was linked to increased sleep quality and reduced myopia risk in humans. Given the well-established role that the PDE11 substrates cAMP and cGMP play in eye physiology and sleep, we determined if (1) PDE11A protein is expressed in the retina or other eye segments in mice, (2) PDE11A-Y7272C affects catalytic activity and/or subcellular compartmentalization more so than the nearby suicide-associated PDE11A-M878V variant, and (3) Pde11a deletion alters eye growth or sleep quality in male and female mice. Western blots show distinct protein expression of PDE11A4, but not PDE11A1-3, in eyes of Pde11a WT, but not KO mice, that vary by eye segment and age. In HT22 and COS-1 cells, PDE11A4-Y727C reduces PDE11A4 catalytic activity far more than PDE11A4-M878V, with both variants reducing PDE11A4-cAMP more so than PDE11A4-cGMP activity. Despite this, Pde11a deletion does not alter age-related changes in retinal or lens thickness or axial length, nor vitreous or anterior chamber depth. Further, Pde11a deletion only minimally changes refractive error and sleep quality. That said, both variants also dramatically alter the subcellular compartmentalization of human and mouse PDE11A4, an effect occurring independently of dephosphorylating PDE11A4-S117/S124 or phosphorylating PDE11A4-S162. Rather, re-compartmentalization of PDE11A4-Y727C is due to the loss of the tyrosine changing how PDE11A4 is packaged/repackaged via the trans-Golgi network. Therefore, the protective impact of the Y727C variant may reflect a gain-of-function (e.g., PDE11A4 displacing another PDE) that warrants further investigation in the context of reversing/preventing sleep disturbances or myopia.

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Section: Pde11a4 Expression In the Eye Changes With Age In A Segment-...supporting

confidence: 63%

Section: Introductionmentioning

confidence: 99%

The Sleep Quality- and Myopia-Linked PDE11A-Y727C Variant Impacts Neural Physiology by Reducing Catalytic Activity and Altering Subcellular Compartmentalization of the Enzyme

Sbornova,

van der Sande,

Milosavljevic

et al. 2023

Cells

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“…It has been shown that blue light at lower doses (≤300 mJ/cm 2 ) triggers the synthesis of hydrogen peroxide in T-cells and affects cell motility [1]. Other studies suggest that blue light changes the redox state of the cell causing oxidative stress and alter mitochondrial activity [2,3] or that green light can change gene expression [4]. As recent technological advances brought televisions, LEDs, and displays, blue light emitters have become a part of our everyday life and blue light is also used for polymerization of dental resin in dentistry [5], its effect on the behavior of cells should be deeply studied.…”

Section: Introductionmentioning

confidence: 99%

Quantitative Phase Dynamics of Cancer Cell Populations Affected by Blue Light

et al. 2020

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Increased exposition to blue light may induce many changes in cell behavior and significantly affect the critical characteristics of cells. Here we show that multimodal holographic microscopy (MHM) within advanced image analysis is capable of correctly distinguishing between changes in cell motility, cell dry mass, cell density, and cell death induced by blue light. We focused on the effect of blue light with a wavelength of 485 nm on morphological and dynamical parameters of four cell lines, malignant PC-3, A2780, G361 cell lines, and the benign PNT1A cell line. We used MHM with blue light doses 24 mJ/cm2, 208 mJ/cm2 and two kinds of expositions (500 and 1000 ms) to acquire real-time quantitative phase information about cellular parameters. It has been shown that specific doses of the blue light significantly influence cell motility, cell dry mass and cell density. These changes were often specific for the malignant status of tested cells. Blue light dose 208 mJ/cm2 × 1000 ms affected malignant cell motility but did not change the motility of benign cell line PNT1A. This light dose also significantly decreased proliferation activity in all tested cell lines but was not so deleterious for benign cell line PNT1A as for malignant cells. Light dose 208 mJ/cm2 × 1000 ms oppositely affected cell mass in A2780 and PC-3 cells and induced different types of cell death in A2780 and G361 cell lines. Cells obtained the least damage on lower doses of light with shorter time of exposition.

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The effects of photobiomodulation on human dermal fibroblasts in vitro: A systematic review

Tripodi

Corcoran

Antonello

et al. 2021

Journal of Photochemistry and Photobiology B: Biology

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The Effect of Phototherapy on Cancer Predisposition Genes of Diabetic and Normal Human Skin Fibroblasts

Cited by 4 publications

References 5 publications

The Sleep Quality- and Myopia-Linked PDE11A-Y727C Variant Impacts Neural Physiology by Reducing Catalytic Activity and Altering Subcellular Compartmentalization of the Enzyme

The Sleep Quality- and Myopia-Linked PDE11A-Y727C Variant Impacts Neural Physiology by Reducing Catalytic Activity and Altering Subcellular Compartmentalization of the Enzyme

Quantitative Phase Dynamics of Cancer Cell Populations Affected by Blue Light

The effects of photobiomodulation on human dermal fibroblasts in vitro: A systematic review

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