The Effect of Photodynamic Therapy with Bacteriochlorin a on Lens Epithelial Cells in a Capsular Bag Model

Tenten, Yasmine van; Schuitmaker, Hans J.; Wolf, Anneke de; Willekens, B.; Vrensen, Gijs F.J.M.; Tassignon, Marie-José

doi:10.1006/exer.2000.0924

Cited by 33 publications

(30 citation statements)

References 22 publications

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“…Recently, PDT with bacteriochlorin a effectively decreased PCO in vitro in a human capsular bag model [4]. Because our study used an in vivo model, we were able to provide further information on the issues which the previous in vitro study could not address.…”

Section: Discussionmentioning

confidence: 67%

“…PCO is caused by the proliferation and migration of lens epithelial cells (LECs) remaining in the capsular bag after cataract surgery [2,3]. Therefore, one of the approaches to prevent postoperative proliferation of LECs is to kill these cells in the capsular bag during surgery [4]. Applying antimetabolites into the capsular bag during phacoemulsification seems to be an effective way of eliminating LECs, but the safety of the drug remaining in the bag and its possible antimetabolic effect on adjacent tissues form a problem for clinical application [5][6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

“…Photodynamic therapy (PDT) consists of light irradiation after the injection of a photosensitizer [4,10]. This procedure can therefore be used in cataract surgery after the removal of the nucleus and cortex.…”

Section: Introductionmentioning

confidence: 99%

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The Effect of Photodynamic Therapy with Rose Bengal on Posterior Capsule Opacification in Rabbit Eyes

Koh

Kang²,

Lim³

et al. 2002

Ophthalmic Res

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We investigated the in vivo effect of photodynamic therapy (PDT) using rose bengal on the development of posterior capsule opacification (PCO). Endocapsular phacoemulsification was performed on white rabbits, which were divided into 4 groups: control group; group 1, treated with visible light only; group 2, treated with rose bengal only, and group 3, treated with PDT. In the case of the PDT group, rose bengal dissolved in sodium hyaluronate was injected into the empty capsular bag and treated with visible light. Three months after surgery, the rabbits were sacrificed and the eyeballs enucleated. The obstruction rate of visible light caused by PCO was measured with an optical powermeter. The mean obstruction rate was 30.6% in the control group, 28.3% in group 1, 19.3% in group 2, and 14.3% in group 3. Group 3 showed a statistically significant decrease in PCO compared with the control group and group 1 (p = 0.0014). Our results suggest that PDT using rose bengal effectively decreased PCO in rabbit eyes.

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Section: Discussionmentioning

confidence: 67%

Section: Introductionmentioning

confidence: 99%

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The Effect of Photodynamic Therapy with Rose Bengal on Posterior Capsule Opacification in Rabbit Eyes

Koh

Kang²,

Lim³

et al. 2002

Ophthalmic Res

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“…[1][2][3][4] The basic principle of PDT for cancer is the combination of a photosensitizing drug capable of absorbing within the body's therapeutic window ( ¼ 620{850 nm), a light source (e.g., a laser) of an appropriate wavelength and molecular oxygen. The photosensitizer, which accumulates preferentially in cancer cells and has a low dark toxicity, is injected into human body tissue and then irradiated with visible light.…”

Section: Introductionmentioning

confidence: 99%

TD-DFT Studies on Hematoporphyrin and Its Dimers

et al. 2008

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A theoretical study has been performed on a hematoporphyrin and its dimers which are components of Photofrin, a photosensitizer. Full geometry optimizations have been carried out using the PBEPBE functional and 6-31G(d) basis set. This combination gives better agreement with X-ray crystal data of porphyrin. Among the dimers studied, the C-C linked structure is found to have the highest stability. The predicted change of free energy (ÁG ¼ À13:9 kcal/mol) suggests that the interconversation of ester to ether would be thermodynamically favorable. The time-dependent density functional theory (TDDFT) studies show that Q-band absorption maxima undergo a less intense transition and low oscillator strength, indicating that dimers have activity when treated under higher dosage.

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“…In a previous study, it was observed that PDT with BCA is able to induce cell death in LECs in vitro [20]. For these experiments, a human lens capsular bag culture system was used, as designed by Liu et al [21].…”

Section: Introductionmentioning

confidence: 99%

A Preliminary Study on the Prevention of Posterior Capsule Opacification by Photodynamic Therapy with Bacteriochlorin <i>A</i> in Rabbits

et al. 2002

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Purpose: Photodynamic therapy (PDT) with bacteriochlorin a (BCA) has proven to be successful in the treatment of cancers and to be cytocidal for lens epithelial cells (LECs) in culture. The present study aimed to determine whether PDT with BCA is also effective in destroying LECs in the capsular bag in vivo and could therefore be a strategy for prevention of posterior capsule opacification (PCO). Materials and Methods: BCA was obtained by saponification and acid hydrolysis of bacteriochlorophyll a and was formulated in 30% polyethylene glycol, 20% ethanol and 50% water. Nine albino rabbits were anesthesized and both pupils dilated. Extracapsular lens extraction by phacoemulsification was performed on both eyes. One eye of each animal served as control. In the other eye, 1.5 ml BCA (10 or 50 µg/ml) was injected in the capsular bag and after 10 min, the eye was illuminated with a diode laser (wavelength 760 nm) for 10 or 15 min. Six weeks after surgery, the rabbits were sacrificed and the globes were enucleated, the capsular bags and the corneas removed, fixed and examined using stereomicroscopy and light microscopy. Results: In the control capsular bags, extensive proliferation of LECs and formation of a complete ring of Soemmering was found, while in the PDT-treated capsular bags, LEC proliferation was markedly diminished and an incomplete irregular and much thinner ring of Soemmering was formed. Using the presently described application, the corneas of the PDT-treated animals were opaque and swollen and had lost their endothelial lining. Conclusion: PDT with BCA induces cell death in LECs and greatly reduces the formation of a ring of Soemmering. Therefore, it could be a promising novel means of prevention of PCO, provided the total length of the treatment can be substantially reduced and the negative effects on corneal transparency avoided.

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The Effect of Photodynamic Therapy with Bacteriochlorin a on Lens Epithelial Cells in a Capsular Bag Model

Cited by 33 publications

References 22 publications

The Effect of Photodynamic Therapy with Rose Bengal on Posterior Capsule Opacification in Rabbit Eyes

The Effect of Photodynamic Therapy with Rose Bengal on Posterior Capsule Opacification in Rabbit Eyes

TD-DFT Studies on Hematoporphyrin and Its Dimers

A Preliminary Study on the Prevention of Posterior Capsule Opacification by Photodynamic Therapy with Bacteriochlorin <i>A</i> in Rabbits

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