The Effect of Phlebotomy and Mannitol on Acute Renal Injury Induced by Ischemia/Reperfusion of Lower Limbs in Rats

Gökşin, İbrahim; Adalı, Fahri; Enli, Yaşar; Akbulut, Metin; Teke, Zafer; Sackan, Gokhan; Ocak, Erkin; Özcan, Ali

doi:10.1016/j.avsg.2011.07.007

Cited by 22 publications

(13 citation statements)

References 43 publications

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“…In previous studies investigating the effects of different agents including PDE-5 inhibitors, several authors showed that there was a considerable decrease of damage in treatment groups compared to the I/R groups [1617242526]. In our study, the histopathological renal damage in the early period was found to be higher in the I/R, mannitol and udenafil groups when compared to the control group.…”

Section: Discussionsupporting

confidence: 58%

The renoprotective effects of mannitol and udenafil in renal ischemia-reperfusion injury model

et al. 2017

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PurposeThe aim of this study was to investigate and compare the effects of udenafil and mannitol in an experimental renal ischemia-reperfusion (I/R) injury model.Materials and MethodsA total of 64 female Wister Albino rats were used. Right nephrectomy was performed in all groups. In the control group; I/R injury was not performed. In the I/R group; left renal pedicle was clamped for 45 minutes and then underwent 60 minutes and 24 hours of reperfusion. In the mannitol group; 1 mL 20% mannitol was given intravenously 15 minutes before clamping. In the udenafil group; 10-mg/kg udenafil was given orally 1 hour before clamping. Creatinine (Cr), blood urea nitrogen (BUN), Cr clearance, malondialdehyde, neutrophil gelatinase associated lipocalin (NGAL), histological examination and DNA damage (Comet Assay method) levels were compared in tissue, serum and urine samples.ResultsUdenafil had a better protective effect than mannitol according to biochemical parameters (Cr, BUN, Cr clearance, and NGAL levels) and histopathological findings when compared with the I/R group. In the Comet sampling analysis no significant difference was detected.ConclusionsUdenafil has a better renoprotective effect than mannitol against I/R injury and this effect supports more functional improvements. Further clinical trials are needed to demonstrate those effects and clinical utility of udenafil for that purpose in humans.

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Section: Discussionsupporting

confidence: 58%

The renoprotective effects of mannitol and udenafil in renal ischemia-reperfusion injury model

et al. 2017

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“…Indeed, healthy, young rats are extremely robust and resilient and previous studies subjected animals to 3 or more hours of ischemia to ensure a significant degree of tissue injury. 24,26 Overall, this study adds further evidence to support the role of hydrogen sulfide as an agent that is protective against inflammation in IRI.…”

Section: Discussion the Role Of Hydrogen Sulfide In A Rodent Model Ofmentioning

confidence: 78%

“…Many of the rodent models use durations of up to 3 hr of lower limb ischemia, which results in a greater degree of injury but does not accurately represent the clinical scenario. 24,26 There is evidence that a shorter duration of ischemia leads to enough remote renal injury to demonstrate a difference between control and treatment groups. 25 In addition, in the clinical settings, other factors such as blood loss, hypotension, preexisting renal disease, and comorbidities such as diabetes and cardiac events would also have an impact on renal injury.…”

Section: Discussion the Role Of Hydrogen Sulfide In A Rodent Model Ofmentioning

confidence: 98%

Hydrogen Sulfide Reduces Inflammation Following Abdominal Aortic Occlusion in Rats

Hunter

Hosgood

Patel

et al. 2015

Annals of Vascular Surgery

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“…Oxidative stress and the released free-radicals have important roles in renal dysfunction following lower limb IR [54], [55]. There are two main pathways of forming free-radicals: by way of oxidative and nitrosative reactions.…”

Section: Discussionmentioning

confidence: 99%

Attenuation of Skeletal Muscle and Renal Injury to the Lower Limb following Ischemia-Reperfusion Using mPTP Inhibitor NIM-811

et al. 2014

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IntroductionOperation on the infrarenal aorta and large arteries of the lower extremities may cause rhabdomyolysis of the skeletal muscle, which in turn may induce remote kidney injury. NIM-811 (N-metyl-4-isoleucine-cyclosporine) is a mitochondria specific drug, which can prevent ischemic-reperfusion (IR) injury, by inhibiting mitochondrial permeability transition pores (mPTP).ObjectivesOur aim was to reduce damages in the skeletal muscle and the kidney after IR of the lower limb with NIM-811.Materials and methodsWistar rats underwent 180 minutes of bilateral lower limb ischemia and 240 minutes of reperfusion. Four animal groups were formed called Sham (receiving vehicle and sham surgery), NIM-Sham (receiving NIM-811 and sham surgery), IR (receiving vehicle and surgery), and NIM-IR (receiving NIM-811 and surgery). Serum, urine and histological samples were taken at the end of reperfusion. NADH-tetrazolium staining, muscle Wet/Dry (W/D) ratio calculations, laser Doppler-flowmetry (LDF) and mean arterial pressure (MAP) monitoring were performed. Renal peroxynitrite concentration, serum TNF-α and IL-6 levels were measured.ResultsLess significant histopathological changes were observable in the NIM-IR group as compared with the IR group. Serum K+ and necroenzyme levels were significantly lower in the NIM-IR group than in the IR group (LDH: p<0.001; CK: p<0.001; K+: p = 0.017). Muscle mitochondrial viability proved to be significantly higher (p = 0.001) and renal function parameters were significantly better (creatinine: p = 0.016; FENa: p<0.001) in the NIM-IR group in comparison to the IR group. Serum TNF-α and IL-6 levels were significantly lower (TNF-α: p = 0.003, IL-6: p = 0.040) as well as W/D ratio and peroxynitrite concentration were significantly lower (p = 0.014; p<0.001) in the NIM-IR group than in the IR group.ConclusionNIM-811 could have the potential of reducing rhabdomyolysis and impairment of the kidney after lower limb IR injury.

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The Effect of Phlebotomy and Mannitol on Acute Renal Injury Induced by Ischemia/Reperfusion of Lower Limbs in Rats

Cited by 22 publications

References 43 publications

The renoprotective effects of mannitol and udenafil in renal ischemia-reperfusion injury model

The renoprotective effects of mannitol and udenafil in renal ischemia-reperfusion injury model

Hydrogen Sulfide Reduces Inflammation Following Abdominal Aortic Occlusion in Rats

Attenuation of Skeletal Muscle and Renal Injury to the Lower Limb following Ischemia-Reperfusion Using mPTP Inhibitor NIM-811

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