The effect of phenols on the parameters of chlorophyll fluorescence and reactions of P700 in green algae Scenedesmus quadricauda

Маторин, Д. Н.; Плеханов, С. Е.; Bratkovskaya, L. B.; Yakovleva, O. V.; Alekseev, A. A.

doi:10.1134/s0006350914030166

Cited by 9 publications

(10 citation statements)

References 11 publications

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“…Phenol and its derivatives are thought to specifically inhibit the Q i site [ 29 ]. Phenol also reportedly acts as an uncoupler of phosphorylation or an inhibitor of electron transfer in photosystems, depending on the concentration [ 30 ]. The I 0 appeared to be due primarily to the re-oxidation of DCBQ that had been reduced by cytochrome b L or ubiquinone in the Q i site.…”

Section: Resultsmentioning

confidence: 99%

An Evaluation of Sensor Performance for Harmful Compounds by Using Photo-Induced Electron Transfer from Photosynthetic Membranes to Electrodes

Kasuno

Kimura

Yasutomo

et al. 2016

Sensors

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Rapid, simple, and low-cost screening procedures are necessary for the detection of harmful compounds in the effluent that flows out of point sources such as industrial outfall. The present study investigated the effects on a novel sensor of harmful compounds such as KCN, phenol, and herbicides such as 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), 2-chloro-4-ethylamino-6-isopropylamino-1,3,5-triazine (atrazine), and 2-N-tert-butyl-4-N-ethyl-6-methylsulfanyl-1,3,5-triazine-2,4-diamine (terbutryn). The sensor employed an electrode system that incorporated the photocurrent of intra-cytoplasmic membranes (so-called chromatophores) prepared from photosynthetic bacteria and linked using carbon paste electrodes. The amperometric curve (photocurrent-time curve) of photo-induced electron transfer from chromatophores of the purple photosynthetic bacterium Rhodobacter sphaeroides to the electrode via an exogenous electron acceptor was composed of two characteristic phases: an abrupt increase in current immediately after illumination (I0), and constant current over time (Ic). Compared with other redox compounds, 2,5-dichloro-1,4-benzoquinone (DCBQ) was the most useful exogenous electron acceptor in this system. Photo-reduction of DCBQ exhibited Michaelis-Menten-like kinetics, and reduction rates were dependent on the amount of DCBQ and the photon flux intensity. The Ic decreased in the presence of KCN at concentrations over 0.05 μM (=μmol·dm−3). The I0 decreased following the addition of phenol at concentrations over 20 μM. The Ic was affected by terbutryn at concentrations over 10 μM. In contrast, DCMU and atrazine had no effect on either I0 or Ic. The utility of this electrode system for the detection of harmful compounds is discussed.

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Section: Resultsmentioning

confidence: 99%

An Evaluation of Sensor Performance for Harmful Compounds by Using Photo-Induced Electron Transfer from Photosynthetic Membranes to Electrodes

Kasuno

Kimura

Yasutomo

et al. 2016

Sensors

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“…The phenol groups attached to the main structure of tannic acid could be another reason as to why TA is capable of P. minimum growth decline. Phenols have the capacity to inhibit electron transport in photosystem II while the share of Q B -nonreducing centers is increased [ 62 ]. Meanwhile, Nakai et al .…”

Section: Discussionmentioning

confidence: 99%

Physiological and Molecular Response of Prorocentrum minimum to Tannic Acid: An Experimental Study to Evaluate the Feasibility of Using Tannic Acid in Controling the Red Tide in a Eutrophic Coastal Water

Jeong

Malazarte

et al. 2016

IJERPH

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Bioassay and gene expression experiments were conducted in order to evaluate the growth and physiology of Prorocentrum minimum isolated from a eutrophic coastal water in response to tannic acid. In the bioassay experiments, variations in abundance, chlorophyll (chl) a concentration, maximum fluorescence (in vivo Fm), and photosynthetic efficiency (Fv/Fm) were measured over the course of a seven-day incubation. Moreover, stress-related gene expression in both the control and an experimental (2.5 ppm TA treatment) group was observed for 24 h and 48 h. The molecular markers used in this study were the heat shock proteins (Hsp70 and Hsp90) and cyclophilin (CYP). The findings show that P. minimum can thrive and grow at low concentrations (<2.5 ppm) of tannic acid, and, above this concentration, cells begin to slow down development. In addition, TA concentration of 10 ppm halted photosynthetic activity. At the molecular level, treatment with tannic acid increased the expression of Hsp70, Hsp90, and CYP, and heat shock proteins are more upregulated than the cyclophilin gene. Exposure to tannic acid increased the expression of stress factors over time (48 h) by 10- to 27-fold the expression level of the control group. These results suggest that tannic acid can be used to control harmful algal blooms such as those containing P. minimum in eutrophic coastal waters.

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“…Several studies have reported that the structure of algal cells can be affected by the existence of phenol; for instance, the membranes of algal cells may be damaged by hydrophobic interaction with lipid bilayer structures [9,10]. Additionally, photosynthetic systems of algal cells might be damaged by phenol, as recent studies have shown that phenol changes their PSII structures and functions [10,11]. However, most studies have focused on the effects of phenol on the growth and physiology of single algal species in mono-cultures [11,12,13], while there is a lack of information on its influence on interspecific competition and dominance establishment.…”

Section: Introductionmentioning

confidence: 99%

Effects of Phenolic Pollution on Interspecific Competition between Microcystis aeruginosa and Chlorella pyrenoidosa and their Photosynthetic Responses

Tan

Dai

Parajuli

et al. 2019

IJERPH

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The demand for phenolic compounds has been increasing rapidly, which has intensified the production and usage of phenol at a commercial scale. In some polluted water bodies, phenol has become one of the typical aromatic contaminants. Such water bodies are inescapably influenced by nutrients from human activities, and also suffer from nuisance cyanobacterial blooms. While phenolic pollution threatens water safety and ecological balance, algal cells are ubiquitous and sensitive to pollutants. Therefore, effects of phenolic pollution on interspecific competition between a bloom-forming cyanobacterium and other common alga merit quantitative investigation. In this study, the effects of phenol on Microcystis aeruginosa (M. aeruginosa, a bloom-forming cyanobacterium) and Chlorella pyrenoidosa (C. pyrenoidosa, a ubiquitous green alga) were analyzed in mono- and co-cultures. The two species were exposed to a series of phenol treatments (0, 2, 20, and 200 μg mL−1). Population dynamics were measured by a flow cytometer and analyzed by the Lotka-Volterra model. The results showed that M. aeruginosa was more sensitive to phenol (EC50 = 80.8 ± 0.16 μg mL−1) compared to C. pyrenoidosa (EC50 = 631.4 ± 0.41 μg mL−1) in mono-cultures. M. aeruginosa won in the co-cultures when phenol was below or equal to 20 μg mL−1, while C. pyrenoidosa became the dominant species in the 200 μg mL−1 treatment. Photosynthetic activity was measured by a fluometer. Results showed phenol significantly impacted the photosynthetic activity of M. aeruginosa by inhibiting the acceptor side of its photosystem II (PSII), while such inhibition in C. pyrenoidosa was only observed in the highest phenol treatment (200 μg mL−1). This study provides a better understanding for predicting the succession of algal community structure in water bodies susceptible to phenolic contamination. Moreover, it reveals the mechanism on photosynthetic responses of these two species under phenolic stress.

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The effect of phenols on the parameters of chlorophyll fluorescence and reactions of P700 in green algae Scenedesmus quadricauda

Cited by 9 publications

References 11 publications

An Evaluation of Sensor Performance for Harmful Compounds by Using Photo-Induced Electron Transfer from Photosynthetic Membranes to Electrodes

An Evaluation of Sensor Performance for Harmful Compounds by Using Photo-Induced Electron Transfer from Photosynthetic Membranes to Electrodes

Physiological and Molecular Response of Prorocentrum minimum to Tannic Acid: An Experimental Study to Evaluate the Feasibility of Using Tannic Acid in Controling the Red Tide in a Eutrophic Coastal Water

Effects of Phenolic Pollution on Interspecific Competition between Microcystis aeruginosa and Chlorella pyrenoidosa and their Photosynthetic Responses

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