The Effect of Phaseoli Semen Herbal-acupuncture at KI10 in Lipopolysaccharide Induced Acute Nephritis in Rats

Abstract

Objectives : This study was designed to evaluate the effects of Phaseoli Semen Herbalacupuncture(PS-HA) at KI10 in acute nephritis induced by lipopolysaccharide(LPS) in rat. Methods : The rats were divided into 5 groups, which were control, LPS, PS-HA, NP and saline group. LPS, PS-HA, NP and saline groups were given LPS to induce acute nephritis and control group did not receive LPS. LPS group did not receive any treatment after the onset of acute nephritis. PS-HA, NP and saline group received PS-HA, normal acupuncture, and saline injection at KI10 three times per week, respectively. To evaluate the effect of PS-HA at KI10, the complete blood count, BUN, creatinine, TNF-α, and CINC-1 in serum were measured. To show its effect on renal function, creatinine, and total protein in urine was measured as well as urine output. The level of myeloperoxidase in renal tissue was quantified and complete histology was done in kidney samples obtained from the rats. Results : PS-HA group showed a significant reduction in the proportion of WBC and neutrophil, serum BUN, TNF-a, and CINC-1 compared to LPS group. Furthermore, a significant increase in urine output and a decrease in urinary creatinine level, MPO in renal tissue, and number of neutrophils at glomerulus was observed in PS-HA group compared to LPS group Conclusions : PS-HA at KI10 was shown to have a significantly effect on treating LPS induced acute nephrits. Therefore, future study is needed to further evaluate the clinical usefulness of PS-HA at KI10 in treating acute nephritis.

The Effect of Herbal-acupuncture at KI on Lipopolysaccharide Induced Nephritis in Rats

“…한의학적으로는 ≪黃帝內經≫에서 "腎者主水, 受五藏六府之精而藏之, 故五藏盛, 乃能寫" 4) , "腎者, 作强之官, 伎巧出焉 . Kim et al 17) , Park et al 18) , Kwak et al 19) 에 , Park et al 18) , Kwak et al 19) 의 (Fig. 2).…”

“…기존의 한의학적 실험 연구로는 구맥 17) ㆍ차전자 18) ㆍ적소 두 19) 약침을 음곡 상응부위에 시행하여 유효한 효과를 보인 보고가 있다. 백복령의 신장염에 관련된 연구로는 Moon et al 20) 의 白茯苓 전탕액 배수혈 약침이 정상 흰쥐의 신장 기 능에 미치는 영향에 대한 연구, Yoo et al 21) 의 白茯苓 전탕 …”

The Effect of Pharmacopuncture at KI in Lipopolysaccharide Induced Acute Nephritis in Rats

“…기존 신장의 손상에 관한 연구로는 Ju et al 9) , Lee et al 10) , Lee et al 11) , Yu et al 12) , Lee et al 13) , Kim et al 14), Kim et al 15) , Jo et al 16) 의 단일약물 및 복합제제를 이용 한 연구와, Park et al 17) , Lee et al 18) , Yoon et al 19) , Her et al 20) , Cho et al 21) , Kwak et al 22) 의 여러 약침을 음곡에 시술하여 신장염에 유의한 효과를 보인 연구가 있었으나, 비 해를 이용하여 신장염에 적용한 연구는 아직 접하지 못하 였다.…”