The effect of ph on contractility of porcine renal and mesenteric arteries in vitro

The 2023 Sir David Cuthbertson Lecture. A fluid journey: Experiments that influenced clinical practice

“…The next aim was to determine if these contractions were sodium-dependent or chloride-dependent. We used similar concentrations of sodium gluconate, but this did not produce a concentration-dependent increase in contractility, confirming that the high chloride concentration was responsible for this [61].…”

“…We first used isolated porcine renal arteries and showed that if we placed them in a bath containing increasing concentrations of KCl there was an almost linear relationship between concentration and contraction. We then used increasing concentrations of NaCl and found a similar, albeit smaller degree of contraction, even at clinically relevant concentrations of chloride [61]. The concentrationdependent contractions were greater in the renal than in the mesenteric arteries.…”

“…In a recent in vitro experiment published in abstract form we sought to ask the question if there was a vascular component to acute kidney injury caused by hyperchloraemic acidosis [61]. We first used isolated porcine renal arteries and showed that if we placed them in a bath containing increasing concentrations of KCl there was an almost linear relationship between concentration and contraction.…”