The Effect of pH on Human Platelet Aggregation Induced by Epinephrine and ADP

Rogers, Anne B.

doi:10.3181/00379727-139-36307

Cited by 45 publications

(26 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, when changes o f similar magnitude occurred under both storage conditions as they did for ADP, epinephrine and collagen, they are not likely attributable to pH change. Indeed, in the present study, the rate o f ADP and 2° epinephrine-induced aggregation decreased with time in the face o f an increasing pH, while Lamberth et al (1974) and Rogers (1972) reported that this parameter increased with increasing pH over a similar pH range. However, our finding of an increase in the rate o f 1 ° epinephrineinduced aggregation in parallel with a decrease in the rate of 2° aggregation is in agreement with the findings o f Warlow et al (1974) who observed similar changes with time in plasma held at constant pH.…”

Section: Discussionsupporting

confidence: 46%

Effect of Temperature on Short-Term Preservation of Platelets for <i>in vitro </i>Tests

Rosenstein¹,

Zacharski²

1978

Pathophysiol Haemos Thromb

View full text Add to dashboard Cite

We have evaluated the effect on the response of citrated platelet-rich plasma to aggregating agents of storage at 4 °C versus room temperature (21 °C) for 2 and 4 h after venipuncture. While there were small decreases in some responses to epinephrine, ADP and collagen attributable to 21 °C storage, only in the case of ristocetin-induced aggregation was a profound difference noted. Platelets stored at 4 °C for 4 h showed no significant change in response to ristocetin. In contrast, those stored at room temperature showed a marked decrease. This change is not likely to be attributable to a change of pH, although pH rose less with storage at 4 °C than at 21 °C. It is recommended that PRP for in vitro testing be stored at 4 °C.

show abstract

Section: Discussionsupporting

confidence: 46%

Effect of Temperature on Short-Term Preservation of Platelets for <i>in vitro </i>Tests

Rosenstein¹,

Zacharski²

1978

Pathophysiol Haemos Thromb

View full text Add to dashboard Cite

show abstract

“…As seen with other agonists [5,[7][8][9][10], this aggregation is very much dependent on pH conditions [ 11].…”

Section: Discussionmentioning

confidence: 68%

Continuous Inhibition of Serotonin-Induced Platelet Aggregation during Chronic Ketanserin Administration to Man Can Be Detected after Plasma pH Control

Arnout¹,

Russelt²,

Deckmyn³

et al. 1987

Pathophysiol Haemos Thromb

View full text Add to dashboard Cite

Ketanserin is a well-known serotonin S₂ receptor antagonist but its capacity to inhibit serotonin-induced aggregation ex vivo during chronic administration has been a matter of debate. In vitro evidence is presented that the inhibitory capacity of ketanserin is lowered by increasing plasma pH. Since the pH of plasma kept at the open air increases with time, we studied the effect of chronic administered ketanserin on serotonin-induced platelet aggregation in plasma kept at a lowered pH of 7.70, by replacing the air with 95% O₂–5% CO₂. Using this slightly modified technique, we could, in contrast to our previous studies, observe a complete inhibition of the serotonin-induced aggregation throughout the ketanserin treatment period.

show abstract

“…The inhibitory activity of local acidosis on platelets has been described long time ago [28]. Low pH was shown to inhibit ADPinduced change in platelet shape, adhesion, spreading and interaction with fibrinogen, fibronectin or collagen [29].…”

Section: Discussionmentioning

confidence: 98%

Improved circulating microparticle analysis in acid-citrate dextrose (ACD) anticoagulant tube

György

Pálóczi

Kovacs

et al. 2014

Thrombosis Research

108

View full text Add to dashboard Cite

Introduction: Recently extracellular vesicles (exosomes, microparticles also referred to as microvesicles and apoptotic bodies) have attracted substantial interest as potential biomarkers and therapeutic vehicles. However, analysis of microparticles in biological fluids is confounded by many factors such as the activation of cells in the blood collection tube that leads to in vitro vesiculation. In this study we aimed at identifying an anticoagulant that prevents in vitro vesiculation in blood plasma samples. Materials and Methods: We compared the levels of platelet microparticles and non-platelet-derived microparticles in platelet-free plasma samples of healthy donors. Platelet-free plasma samples were isolated using different anticoagulant tubes, and were analyzed by flow cytometry and Zymuphen assay. The extent of in vitro vesiculation was compared in citrate and acid-citrate-dextrose (ACD) tubes. Results: Agitation and storage of blood samples at 37°C for 1 hour induced a strong release of both platelet microparticles and non-platelet-derived microparticles. Strikingly, in vitro vesiculation related to blood sample handling and storage was prevented in samples in ACD tubes. Importantly, microparticle levels elevated in vivo remained detectable in ACD tubes. Conclusions: We propose the general use of the ACD tube instead of other conventional anticoagulant tubes for the assessment of plasma microparticles since it gives a more realistic picture of the in vivo levels of circulating microparticles and does not interfere with downstream protein or RNA analyses.© 2013 Elsevier Ltd. All rights reserved. IntroductionExtracellular vesicles (EVs) are membrane surrounded structures of various sizes (30-5000 nm) that have received significant attention recently [1]. EVs may be classified on the basis of their biogenesis, diameter and membrane markers. The two best characterized types of EVs include exosomes of endosomal origin and plasma membrane-derived microparticles (MPs) (recently often referred to also as microvesicles or ectosomes in the literature) [1]. EVs are present in all biological fluids including blood plasma, synovial fluid, cerebrospinal fluid, urine, tears and breast milk [1]. MPs are in between 100 and 1000 nm in diameter, and they are also detectable by flow cytometry [2,3]. Thus, MP profiles are easily analyzed in the routine clinical laboratory practice, and represent novel biomarkers of various diseases. In circulation, most MPs are derived from platelets, red blood cells, endothelial cells and leukocytes. Because of their abundance, platelet-derived MPs (PMPs) received the highest attention during the past few years. Elevated PMP counts are characteristic for nearly all autoimmune disorders [4,5], and also for several cardiovascular and metabolic diseases [1]. EVs not only contain proteins but also RNA molecules [1]. Extracellular RNA (exRNA) in blood plasma is encapsulated in EVs, or bound to either proteins or HDL molecules [6]. exRNAs, particularly miRNAs, are specific and sensitive biomarkers...

show abstract

The Effect of pH on Human Platelet Aggregation Induced by Epinephrine and ADP

Cited by 45 publications

References 1 publication

Effect of Temperature on Short-Term Preservation of Platelets for <i>in vitro </i>Tests

Effect of Temperature on Short-Term Preservation of Platelets for <i>in vitro </i>Tests

Continuous Inhibition of Serotonin-Induced Platelet Aggregation during Chronic Ketanserin Administration to Man Can Be Detected after Plasma pH Control

Improved circulating microparticle analysis in acid-citrate dextrose (ACD) anticoagulant tube

Contact Info

Product

Resources

About