The Effect of pH on Adhesion and Hyphal Formation by Strains of<i>Candida albicans</i>

Shakespeare, Ann P.; Willcox, Mark; Knox, K. W.

doi:10.3109/08910609109140266

Cited by 13 publications

(8 citation statements)

References 30 publications

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“…This observation is in agreement with Persi et al [10] 261 who found that C. albicans yeast cell adherence to vaginal epithelial cells was highly dependent upon the pH and was different for two clinical isolates. However, more recent work did not demonstrate an effect of pH on the adherence process of C. albicans to acrylic [11].…”

Section: Discussionmentioning

confidence: 86%

The contribution of electrostatic forces to the process of adherence of Candida albicans yeast cells to substrates

Klotz¹

1994

FEMS Microbiology Letters

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The contribution of electrostatic interactions to the adherence process of Candida albicans and other Candida species was investigated by mixing cationic or anionic exchange resins possessing free -COO- or -NH+4 groups, respectively, on their surface. The adherence process of yeast cells to the anionic exchange resin is a saturable event that is essentially complete by 60 min. There is no measurable interaction of yeast cells with the cationic exchange resin. All clinical isolates of C. albicans, C. pseudotropicalis, one isolate each of C. tropicalis and Torulopsis glabrata possessed electrostatic charge as defined by this method, whereas two clinical isolates of C. parapsilosis, C. krusei and one isolate of C. tropicalis did not have measurable electrostatic surface charge. The adherence process to the exchange resin with the free -NH+4 group was dependent upon the pH of the suspending medium and varied from one isolate to another. Fixing yeast cells, or alternatively, endothelial cells, in such a manner as to change the surface charge of both and then measuring adherence of yeast cells to the target cells was an event that was not controlled by electrostatic interactions as they are defined herein. It appears that whatever contribution electrostatic charges make to the adherence process, that at best, it is a minor contribution.

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Section: Discussionmentioning

confidence: 86%

The contribution of electrostatic forces to the process of adherence of Candida albicans yeast cells to substrates

Klotz¹

1994

FEMS Microbiology Letters

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“…yeast cells used in individual assays and the sucrose concentrations of the media increased the adherence of yeast in vitro (Verran et al, 1991). This work was undertaken on perspex with a uniform smooth surface and measured by means of an automated image analysis system (Magi-scan 2, Joyce Loebel Ltd, Gateshead, UK).…”

Section: (62) Later Studies (1980 To the Present)mentioning

confidence: 99%

Denture Plaque and Adherence of Candida Albicans To Denture-Base Materials in Vivo and in Vitro

Radford

Challacombe

Walter

1999

Critical Reviews in Oral Biology & Medicine

298

249

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The aim of this paper is to review our understanding of the mechanisms and clinical significance of adhesion of C. albicans to denture-base materials in relation to denture plaque and denture-related stomatitis. Earlier reports in the literature of a 65% prevalence level of denture-related stomatitis seem to be exaggerated. More recent studies indicate that denture-related stomatitis is considerably less common, particularly in normal healthy subjects. The etiology of the condition is discussed in this review, and although much of the literature supports the view that the condition is strongly associated with C. albicans, this is not always so. In some subjects, the cause appears to be related to a non-specific plaque. This review also considers the role of denture plaque in the pathogenesis of denture-related stomatitis, the sequential development of denture plaque, and its colonization by Candida organisms. Designing controlled in vivo studies is difficult, and as a consequence, many investigators have had to resort to in vitro studies. The majority of these studies have attempted to investigate the hydrophobicity of C. albicans, relating the surface free-energy of denture-base materials, particularly acrylic resin, to that of the organism. Surprisingly little work has been directed at surface roughness and how it affects retention of organisms. Further, no attention has been paid to the properties and character of the surface, other than average surface roughness, as it affects adhesion. A comparison of results from in vitro studies on the effect on adhesion of pre-coating the surfaces of denture-base materials with saliva has produced equivocal conclusions. This is largely due to little standardization of experimental protocols between studies, particularly in the collection and handling of the saliva used. In conclusion, the review strongly supports the suggestion that adherence of C. albicans to denture-base materials in vitro is related to the hydrophobicity of the organism. The clinical significance of the observation and the mechanisms for the development and maturation of denture plaque are yet to be understood. There is a clear need for further investigation of other factors that may moderate the adhesion of organisms and subsequent colonization of denture-base materials.

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“…16 The effect of pH on the adhesion of C. albicans strains to mucosal surfaces has been shown to vary with the source of the mucosal cell and the strain. A recent study by Verran et al 18 has shown that C. albicans strains appeared to behave differently in response to a change of pH and that all strains were capable of adhering to buccal epithelial cells (BEC) at pH 7.3, 6.0 and 2.6, although adhesion was low. However, adhesion to BEC was increased when stationary phase cells were used instead of early exponential phase cells, with one strain producing hyphae at pH 2.6.…”

Section: Phmentioning

confidence: 99%

“…2 The same study also showed that at temperatures below 30°C and pH less than 6.0 most C. albicans strains on nutrient-poor media can form very long hyphae over a period of several days. 2 Verran et al 18 demonstrated that at pH 2.6, adherent C. albicans were able to produce hyphae but suspended candida cells did not have this ability. A calcium-calmodulin interaction was shown to induce blastospore-hyphae transition, whereas u n r e s t ricted calcium uptake resulted in specific inhibition of C. albicans hyphal growth 47,48 while another study demonstrated that carbon dioxide alone can induce germ tube form ation in C. albicans.…”

Section: Hyphaementioning

confidence: 99%

Candida‐associated denture stomatitis. Aetiology and management: A review: Part1. Factors influencing distribution of candida species in the oral cavity

Webb

Thomas

Willcox

et al. 1998

Australian Dental Journal

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204

162

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Candida species are yeasts and within the oral cavity, Candida albicans is the most frequently isolated. There is clear evidence that C. albicans adheres to oral surfaces including acrylic dentures and mucosa. The mechanisms of attachment differ, with candidal adhesion to inert surfaces under the control of hydrophobic and electrostatic forces and adhesion to mucosa dependent on a number of complex ligand-recognition systems. Other factors within the oral environment such as saliva, pH, bacteria and hyphal formation have been shown to influence adhesion of candida species to surfaces in the mouth.

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The Effect of pH on Adhesion and Hyphal Formation by Strains ofCandida albicans

Cited by 13 publications

References 30 publications

The contribution of electrostatic forces to the process of adherence of Candida albicans yeast cells to substrates

The contribution of electrostatic forces to the process of adherence of Candida albicans yeast cells to substrates

Denture Plaque and Adherence of Candida Albicans To Denture-Base Materials in Vivo and in Vitro

Candida‐associated denture stomatitis. Aetiology and management: A review: Part1. Factors influencing distribution of candida species in the oral cavity

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