The effect of perfusion with UW solution on the skeletal muscle and vascular endothelial exocrine function in rat hindlimbs

Preservation of Amputated Extremities by Extracorporeal Blood Perfusion; a Feasibility Study in a Porcine Model

“…The use of hemoglobin was thereafter abandoned and extremities were washed out [33,34], stored [35][36][37], perfused intermittently [34,38] or continuously [38][39][40] with fluids of different compositions, including physiological saline [41], Ringer Lactate [39], compositions using mannitol [42], and the standard organ preservation solutions University of Wisconsin [34,35,40,[43][44][45] and Euro Collins [33,39,43,45]. To increase the oxygenation of the perfused extremity, artificial oxygen carriers like Fluosol [36,38,46] and Fluorocarbon [38] have been used and one study even investigated the effect of hyperbaric oxygen chambers [47].…”

The A3Adenosine Receptor: History and Perspectives

“…Therefore protecting skeletal muscle from IR insult is an important aim of therapy to ameliorate muscle and organ injury (Tsuchida et al, 2003).…”

Extracorporeal perfusion of isolated organs of large animals – Bridging the gap between in vitro and in vivo studies

“…The former distinguishes itself by its relatively high glucose content (214 mmol/l) and is best suited for lung preservation (Hicks et al, 2006), while the latter is characterized by three impermeants (lactobionate, raffinose, and hydroxyethyl starch) and is well-matched for kidney, liver and pancreas preservation (Hicks et al, 2006). Both solutions are unsuitable for the preservation of muscle and extremities (Tsuchida et al, 2001(Tsuchida et al, , 2003.…”

“…Comparing replantation of amputated rat hind limbs after perfusion with the superior set-up (University of Wisconsin solution, high perfusate pressure) with replantation of non-perfused limbs, outcomes with perfusion were only slightly superior. An observation that might be explained by the potential deterioration of vascular endothelial function and consequently blood flow with the use of University of Wisconsin solution (Tsuchida et al, 2003).…”

“…As discussed above, half of this reported total weight gain occurred during the initial wash out period (20 to 30 minutes) with cold Ringer's solution, illustrating that this perfusate solution does not maintain oncotic pressure sufficiently . Human liver perfusion with Krebs bicarbonate buffer containing 20% prewashed human or bovine red blood cells, bovine serum albumin, α1-acid-glycoprotein, calcium and glucose, how-1924;Newton, 1933). This technique was later replaced by the availability of artificial oxygenation devices (Hardwick and Linzell, 1960).…”