The effect of penicillin administration in early life on murine gut microbiota and blood lymphocyte subsets

Daniluk, Jarosław; Daniluk, Urszula; Rusak, Małgorzata; Dąbrowska, Milena; Reszeć, Joanna; Garbowicz, Magdalena; Humińska, Kinga; Dâbrowski, A

doi:10.1016/j.anaerobe.2017.03.015

Cited by 7 publications

(4 citation statements)

References 25 publications

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“…Alongside well-known in vitro effects of a wide range of antibiotics on granulocyte chemotaxis and lymphocyte function, this may also indicate the likelihood of treatment associated alterations in peripheral blood neutrophil counts that may reflect the selective increase in bacteria populations not covered by the prescribed antibiotic regimen. Notably, in mice treated with antibiotic effective against gram-positive bacteria, selective increase of gram negative populations of bacteria in colon and stool was shown to be accompanied by the increased percentage of neutrophils in peripheral blood of antibiotic treated mice as compared with the control mice [28].…”

Section: Discussionmentioning

confidence: 96%

Neutrophil-to-Lymphocyte Ratio as a Potential Early Marker of Antibiotic Resistance in Patients with Infected Cirrhotic Ascites

Şahintürk¹,

Çekın²

2018

Clin. Lab.

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Background: To evaluate the role of neutrophil-to-lymphocyte ratio (NLR) in identification and management of infected ascites among patients with cirrhosis. Methods: A total of 439 patients (mean (SD) age: 64.5 (± 12.7) years, 63.3% were males) hospitalized with cirrhotic ascites were included in this retrospective study. Data on patient demographics, etiology of cirrhosis, type of ascites (sterile ascites, infected ascites), culture findings treatment response (antibiotic resistance vs. sensitivity) and baseline (Day 0), Day 1 and Day 2 levels for serum C-reactive protein (CRP; mg/L), and NLR were recorded. Receiver operating characteristics (ROC) curve was plotted to determine performance of % change from baseline NLR on Day 1 in identifying treatment response. Results: In patients with infected ascites, antibiotic resistant patients had significantly higher Day 1 (6.9 (1.9-74.9) vs. 4.9 (1.1-51.1), p = 0.001) and Day 2 (8.0 (2.6-75.9) vs. 4.0 (1.1-40.3), p = 0.000) levels for NLR as compared with antibiotic sensitive patients, while the two groups had similar baseline (Day 0) NLR values (5.8 (1.1-62.3) vs. 5.7 (1.1-72.3), p = 0.969). ROC analysis revealed less than 0.93% decrease from baseline NLR on Day 1 (AUC (95% CI): 0.852 (0.799-0.895), p < 0.001) to be a potential marker of antibiotic resistance with a sensitivity of 87.72% and specificity of 88.50%. Conclusions: Our findings indicate percent change from baseline NLR on Day 1 to be a potential early marker of antibiotic resistance in patients with infected cirrhotic ascites. Our findings emphasize the role of determining NLR levels in earlier recognition of treatment failure and thus prompt modification of antibiotic treatment in cirrhotic patients with infected ascites.

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Section: Discussionmentioning

confidence: 96%

Neutrophil-to-Lymphocyte Ratio as a Potential Early Marker of Antibiotic Resistance in Patients with Infected Cirrhotic Ascites

Şahintürk¹,

Çekın²

2018

Clin. Lab.

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“…The weight/length ratio of the colon is used as a marker of hyperplasia of the mucosa and of the severity of chronic colitis; because DSS induces a shortening of the colon, this can be used as a visual index and has been used for the study of foods with anti-inflammatory potential (Cuéllar-Núñez et al, 2018;Elimrani et al, 2017). Furthermore, a characteristic of intestinal inflammation is the thickening and shortening of the large bowel which can be measured in mice as an increase in the colon weight/length ratio, which is and indirect indicator of colonic edema and inflammation (Sydora et al, 2012;Daniluk et al, 2017). Okayasu et al (1990) proposed a model for the induction of chronic ulcerative colitis with DSS and found that the colon of DSS-treated animals was significantly shorter than in non-treated animals.…”

Section: Discussionmentioning

confidence: 99%

Protocol for short-term tumor development, as an option for the study of chemopreventive agents

Cid-Gallegos

Sánchez‐Chino

Álvarez-González

et al. 2022

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Introduction: Colon cancer diagnosis is usually performed late; so, it is necessary to search for prevention options. In vivo models are an option for the evaluation of chemopreventive agents, which are based mainly on the induction and promotion of carcinogenesis; however, they take a long time. This work aimed to evaluate and propose a carcinogenesis model, with tumor manifestation in a short time to prove the efficacy of chemopreventive compounds. Method: Colon carcinogenesis was induced in three groups (n = 7) male BALB/c mice with azoxymethane (AOM) and dextran sodium sulfate (DSS). The damage was assessed 14 weeks after the induction. Protocols were: 1) P1: two AOM injections and two DSS cycles at 1.5 % for five days, with three resting days between cycles; 2) P2: one AOM injection and two DSS cycles at 2 % for seven days with five rest days, and 3) P3: one AOM injection and two DSS cycles at 2 % for four days, with four resting days. Negative control was used in parallel, P0: with one injection of saline solution and water ad libitum. Weight, disease activity index (DAI), survival, tumor incidence, lipids, and protein oxidation were determined. Results: P2 showed greater severity in the assessed signs (100 % tumor incidence, colon weight/length ratio 101.68 ± 2.99 mg/cm), with low survival (43 %). P1 depicted lower mortality (14 %) and 83 % tumor incidence, without a significant difference to P2. P3 developed the disease but to a lesser degree (33 % tumor incidence). Furthermore, the three protocols showed lipid oxidation (0.4-0.58 ng/μg of protein) and proteins oxidation (0.6-1 ng/μg of protein). The P1 and P3 induction protocols presented less mortality, weight loss, and acceptable DAI, a weight/length ratio higher than the negative control and presence of tumors. Discussion: The use of AOM (10mg/kg) combined with DSS (1.5-2 %) are suitable models to evaluate the carcinogenic effect of compounds of interest, inflammation signs, lipids and proteins oxidation and a survival number adequate to perform the statistical analysis leading to accurate conclusions. Therefore, P1 and P3 are protocols that can be used in chemoprevention assays with satisfactory results.

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“…For example, in a mouse model, intestinal TH17 cells contributed to development of a microbiota that maintained metabolic homeostasis [29]. Mice treated with penicillin demonstrated a elevated percent of B cells,regulatory T cells, T helper 1 (Th1) and T helper 2 (Th2) cells, but a decreased percent of total CD4+ cell [30]. Treg cell numbers are normalized when the microbiota is reconstituted in germ-free mice, and their development appears to be driven by a mixture of clostridial species [31].…”

Section: Discussionmentioning

confidence: 99%

Clinical Correlations of Peripheral Blood Lymphocyte Populations in Sepsis

Zhao¹,

Chen²,

Wu³

et al. 2020

Preprint

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Background Our understanding of sepsis-associated immune impairment is incomplete. The objective of this retrospective study was to investigate correlations of sepsis clinical manifestations with peripheral blood lymphocyte subpopulations in lymphocyte immunity. Methods Twenty individuals without sepsis and eighteen with sepsis were enrolled. Lymphocyte phenotypes (CD3+, CD4+, CD8+, CD3-CD16+CD56+, CD19+, CD4+CD25+CD127+, CD8+CD28-, and CD8+CD28+) were assessed by flow cytometry. Fresh fecal bacteria cue proportion was measured to determine intestinal dysbacteriosis. Results Compared with the non-sepsis group, the sepsis patients had clearly lower proportions of CD3+, CD4+ and CD8+CD28+ cells and substantially higher proportions of CD19+ cells (p<0.05). Among 38 patients with infection, CD4+ cells and CD8+CD28+ cells in a survivor group had significantly higher presence compared with patients who had died (p<0.05), The subgroup analysis results showed that CD4+ cells in the survivor subgroups were higher than those in the deceased subgroups (p<0.05). CD8+CD28+ cells in the non-sepsis survivor subgroup were higher than those in the deceased subgroups (p<0.05).. Bivariate correlation analysis showed that the intestinal dysbacteriosis was significantly correlated with the severity of sepsis and its prognosis (r2=0.2788, p=0.001, r2=0.1764, p=0.009, respectively). CD4+, CD19+, and CD8+CD28+ cells were significantly correlated with intestinal dysbacteriosis (r2=0.1024, p=0.049, r2=0.1063, p=0.046, r2=0.1909, p=0.006, respectively). Conclusions In conclusion, the lymphocyte populations of CD3+, CD4+, CD8+CD28+ and CD19+ cells were accessible for predicting the severity and mortality of sepsis patients. In addition, intestinal dysbacteriosis had a significant impact on the immune system of sepsis patients as revealed by peripheral blood lymphocyte population.

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The effect of penicillin administration in early life on murine gut microbiota and blood lymphocyte subsets

Cited by 7 publications

References 25 publications

Neutrophil-to-Lymphocyte Ratio as a Potential Early Marker of Antibiotic Resistance in Patients with Infected Cirrhotic Ascites

Neutrophil-to-Lymphocyte Ratio as a Potential Early Marker of Antibiotic Resistance in Patients with Infected Cirrhotic Ascites

Protocol for short-term tumor development, as an option for the study of chemopreventive agents

Clinical Correlations of Peripheral Blood Lymphocyte Populations in Sepsis

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