The effect of Pasteurella haemolytica cytotoxin on bovine polymorphonuclear leukocytes can be attenuated by β-adrenoceptor antagonists

Henricks, Paul A.J.; Binkhorst, G. J.; Drijver, Annet A.; Vliet, H. van der; Nijkamp, Frans P.

doi:10.1016/0378-1135(90)90113-a

Cited by 7 publications

(2 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The mechanism by which leukotoxin activates bovine neutrophils has not been identified. The uptake of calcium is likely an important event (14,27) although there is some evidence for the role of 3-adrenergic receptors in the inhibitory effects of leukotoxin on bovine neutrophils (29). Future studies will address the membrane interaction and intracellular alterations associated with the effects of P. haemolytica leukotoxin on bovine neutrophils.…”

Section: Discussionmentioning

confidence: 99%

Activation of bovine neutrophils by partially purified Pasteurella haemolytica leukotoxin

et al. 1991

View full text Add to dashboard Cite

In this study we developed a new method for the partial purification of Pasteurella haemolytica leukotoxin by size-exclusion high-performance liquid chromatography. The partially purified leukotoxin had a molecular weight of 104,000, as estimated by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and reacted on an immunoblot with an antileukotoxin monoclonal antibody. As expected, high concentrations of the leukotoxin were inhibitory or lethal to bovine neutrophils. Incubation of bovine neutrophils with diluted leukotoxin, however, resulted in significant neutrophil activation that was comparable in magnitude to that obtained with standard activating agents such as opsonized zymosan or zymosan-activated serum. Dilute leukotoxin (1:128 to 1:8,192 dilutions) stimulated an oxidative burst (luminol-dependent chemiluminescence) by bovine neutrophils that was comparable in magnitude to that obtained with opsonized zymosan. Preincubation with leukotoxin did not significantly prime the neutrophils for an enhanced oxidative burst when they were then exposed to opsonized zymosan as a second stimulus. Dilute leukotoxin (1:100 to 1:1,000 dilutions) also stimulated cytoskeletal alterations in bovine neutrophils, as measured by a significant shape change response. Preferential release of secondary granule constituents (lactoferrin) occurred when neutrophils were incubated with 1:100 to 1:500 dilutions of leukotoxin. Significant release of primary granules, as measured by I-glucosaminidase activity, was not observed except at low dilutions (1:20) of leukotoxin that resulted in significant release of cytosolic constituents (i.e., lactate dehydrogenase activity). The neutrophil-activating activity of the leukotoxin was heat labile, unaffected by polymyxin B, and abrogated by a leukotoxinneutralizing monoclonal antibody. These data indicate that P. haemolytica leukotoxin, like the closely related Escherichia coli hemolysin, is a potent neutrophil-activating agent. Leukotoxin-stimulated release of neutrophil oxygen intermediates and granule constituents may contribute to the intense inflammation that characterizes bovine pulmonary pasteurellosis. * Corresponding author. trophil oxidative activity, degranulation, and shape change. Our results indicate that the P. haemolytica leukotoxin, like the E. coli hemolysin, is a potent neutrophil-modulating agent.

show abstract

Section: Discussionmentioning

confidence: 99%

Activation of bovine neutrophils by partially purified Pasteurella haemolytica leukotoxin

et al. 1991

View full text Add to dashboard Cite

show abstract

“…The -adrenoceptor antagonists ALP and PRO were able to attenuate the effect of cytotoxin from Pasteurella haemolytica on the CL response of PMNL. The membrane stabilising characteristics of the -adrenoceptor antagonists used were probably not responsible for the diminished interaction between PMNL and the cytotoxin [243].…”

Section: Bab Drugs and Reactive Oxygen Metabolites (Rom) In Pmnlsmentioning

confidence: 97%

Antiplatelet and Antileukocyte Effects of Cardiovascular,Immunomodulatory and Chemotherapeutic Drugs

Nosáľ¹

2006

CHAMC

View full text Add to dashboard Cite

In vitro and ex vivo interactions of betaadrenoceptor blocking drugs, antihistamines and chloroquine with blood platelets and polymorphonuclear leukocytes resulted in different alterations of regulatory functions of these blood cells. Inhibition of platelet aggregation, arachidonate regulatory pathway, 5-hydroxytryptamine transportation, removal of platelet membrane receptors, inhibition of second messenger pathways at subcellular level and suppression of phagocytosis are indicative of nonreceptor rather than specific receptor interactions. Binding of drugs with biomembranes is reversible depending on the ionic charge of the molecule and hydrophobicity of the bilayer, partition coefficient, pH and pKa of the amphiphilic molecules and other physico-chemical properties of amphiphilic drugs. Alterations in the drug molecule structure alters the drug-phospholipid binding profile. Any change in the metabolism of membrane phospholipids directly or indirectly influences one or more of the important components of the phospholipid-signalling pathway. In addition to changes in phospholipase A, C and D activities, protein kinase C, calmodulin-phosphodiesterase, Ca2+,Mg2+-ATPase, Na+,K+-ATPase and other messengers were found to be changed in cells and tissue after cationic amphiphilic drug (CAD) administration. Although not much has been understood of the mechanism by which some CAD affect immune functions, there are good reasons to suggest that these effects might occur. CADs share sufficient similarities in their structure even though they come from diverse pharmacological classes. CADs affect ion transport, immune functions, tumour growth, serotonin metabolism and several other functions in the body. Extensive therapeutic use and associated side effects have generated a great deal of interest in understanding the nonreceptor interactions with CADs.

show abstract

[54] Identification and assay of RTX family of cytolysins

Lobo¹,

Welch²

1994

Methods in Enzymology

View full text Add to dashboard Cite

The effect of Pasteurella haemolytica cytotoxin on bovine polymorphonuclear leukocytes can be attenuated by β-adrenoceptor antagonists

Cited by 7 publications

References 12 publications

Activation of bovine neutrophils by partially purified Pasteurella haemolytica leukotoxin

Activation of bovine neutrophils by partially purified Pasteurella haemolytica leukotoxin

Antiplatelet and Antileukocyte Effects of Cardiovascular,Immunomodulatory and Chemotherapeutic Drugs

[54] Identification and assay of RTX family of cytolysins

Contact Info

Product

Resources

About