The effect of P2X7R- mediated Ca2+ and MAPK signaling in OPG-induced duck embryo osteoclasts differentiation and adhesive structure damage

Ma, Yonggang; Di, Ran; Zhao, Hongyan; Shi, Xueni; Song, Ruilong; Zou, Hui; Liu, Zongping

doi:10.1016/j.lfs.2022.120337

Cited by 3 publications

(2 citation statements)

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“…Since the discovery of OPG, almost all studies on OPG have focused on its effects on osteoclast formation and bone resorption. In recent years, some researchers have explained the effect of OPG on osteoclast survival and apoptosis [ 5 , 14 , 15 ]. In addition, OPG may induce other death modes of mature osteoclasts to play its protective role.…”

Section: Discussionmentioning

confidence: 99%

“…It was found that OPG transgenic mice showed osteosclerosis, and OPG knockout mice showed severe osteoporosis [ 4 ]. In vitro results showed that OPG also inhibited the maturation and differentiation of osteoclast precursors and inhibited the activity of mature osteoclasts [ 5 ]. The study also found that the cessation of estrogen production in postmenopausal women leads to bone loss and osteoporosis in the elderly.…”

Section: Introductionmentioning

confidence: 99%

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The Mechanism of Osteoprotegerin-Induced Osteoclast Pyroptosis In Vitro

Zhu

Wang

et al. 2023

IJMS

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Osteoprotegerin (OPG) is a new member of the tumor necrosis factor (TNF) receptor superfamily, which can inhibit the differentiation and activity of osteoclasts by binding to nuclear factor kappa B receptor activator (RANK) competitively with nuclear factor kappa B receptor activator ligand (RANKL). The previous experiments found that OPG can induce apoptosis of mature osteoclasts in vitro, which can inhibit the activity of mature osteoclasts, thereby exerting its role in protecting bone tissue. In addition, pyroptosis is a new type of cell death that is different from apoptosis. It is unclear whether OPG can induce mature osteoclast pyroptosis and thereby play its role in protecting bone tissue. In this study, the results showed that compared with the control group, the survival rate of osteoclasts in the OPG group was significantly reduced, and the contents of IL-1β, IL-18, and LDH in the supernatant both increased. Many osteoclast plasma membranes were observed to rupture in bright fields, and OPG induced loss of their morphology. Flow cytometry was used to analyze the pyroptosis rate; OPG significantly increased the osteoclast pyroptosis rate. To further reveal the mechanism of OPG-induced osteoclast pyroptosis, we examined the expression level of pyroptosis-related genes and proteins, and the results found that OPG increased the expression of NLRP3, ASC, caspase-1, and GSDMD-N compared with the control group. In summary, OPG can induce osteoclast pyroptosis, and its mechanism is related to the expression levels of ASC, NLRP3, caspase 1 and GSDMD, which were included in the classical pathway of pyroptosis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%