The Effect of Oxytocin on Reparative Histogenesis in the Periodontium and Mucous Membrane of the Airways (Experimental Study)

Abstract

The aim of the study was to elucidate the role and significance of oxytocin in reparative histogenesis of tissues of various genesis using the example of periodontal tissues and extrapulmonary airways.Material and methods. Periodontitis and tracheobronchitis were simulated on sexually mature white outbred male rats. Periodontitis was simulated by traumatic injury to the periodontium, followed by the wound infection with Staphylococcus aureus. Introduction of oxytocin as an additional tool was used in the treatment of periodontitis. Experimental tracheobronchitis was simulated in rats by intratracheal infection with S. aureus (strains with and without antilactoferrin activity). Paraventricular large-cell nuclei of the hypothalamus were additionally destroyed in the simulated experimental tracheobronchitis (in order to reduce the production of endogenous oxytocin). The resulting material (the periodontium, alveolar processes, trachea and extrapulmonary bronchi) was examined using histological methods of investigation.Results. Analysis of histological sections demonstrated that when oxytocin was used in the complex therapy of experimental periodontitis, the proliferation and cyto-differentiation of fibroblasts, osteoblasts and cementoblasts was intensified, followed by their activation; this resulted in the optimization of reparative histogenesis in the structures of the periodontium and alveolar ridge. In animals with destruction of the paraventricular nuclei of the hypothalamus (against the background of an oxytocin deficiency in the body), a decrease in the reparative potential of the tracheal and bronchial mucosa was observed. Moreover, the severity of the inflammatory process in the mucous membrane of the trachea and bronchi with the introduction of staphylococci with antilactoferrin activity was significantly higher than that of animals that were administered staphylococci without antilactoferrin activity.Conclusion. The results obtained indicate the optimizing and correcting effect of the hypothalamic nonapeptide oxytocin on the reparative regeneration of the studied periodontal and the trachea and bronchi structures.

Morphogenesis of Skin Regenerate and Local Factors of Its Regulation

“…Известные способы ускорения заживления ран, ожогов, травм направлены на стимуляцию биологических потенций клеток, участвующих в репарации. Состояние раневого дефекта на различных стадиях восстановления нарушенной структуры зависит от активности регуляторных факторов локального и дистантного характера действия, способа обработки и клинического ведения раневого дефекта [1][2][3][4][5].…”

“…С помощью пинцета вытягивали кожу через трафарет на 2 мм кнаружи и отрезали ножницами. Подкожная мышца при этом не затрагивается, получается полнослойный лоскут и открытая рана площадью 60-70 мм 2 .…”