The effect of oxygen tension on haemopoietic and fibroblast cell proliferation in vitro

Bradley, T. R.; Hodgson, George; Rosendaal, Martin

doi:10.1002/jcp.1040970327

Cited by 213 publications

(95 citation statements)

References 13 publications

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“…Clonal agar culture was performed in a double-layer agar system using a modification of the method routinely used for the growth of marrow cell colonies in this laboratory and extensively described elsewhere (Bradley et al, 1978 02, 10% CO2 and 83% N2 in sealed plastic boxes. A low, oxygen tension in the gas mixture is used routinely in this laboratory in preference to conventional gas mixtures of CO2 in air, as it has been our experience that cell growth in both agar and liquid cultures is markedly improved under low oxygen tension (Bradley et al, 1978). Colonies of at least 40 cells were counted and sized with a calibrated eye-piece grid using an Olympus SZ III dissecting binocular microscope with transmitted indirect lighting at x 20 magnification.…”

Section: Methodsmentioning

confidence: 99%

Limitations of the clonal agar assay for the assessment of primary human ovarian tumour biopsies

Bertoncello¹,

Bradley²,

Campbell³

et al. 1982

Br J Cancer

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Summary.-114 biopsy specimens from 70 patients with ovarian carcinoma at all stages of disease were submitted for assessment of clonogenic capacity in agar. A highly significant correlation was found between agar clonogenicity and patient survival after biopsy. However, problems related to inherent tumour heterogeneity, quality of sample and tissue disaggregation indicate that this technique may have limited applicability in the routine assessment of patients. Only 41 biopsy specimens (36%) from 31 patients (44.3%o) complied with the prerequisite criteria for agar clonogenic assessment, namely: (a) the confirmed presence of malignant cells in the biopsy, (b) the ability to prepare a single-cell suspension, and (c) adequate viable cell numbers for assay. Furthermore, although the dominant patterns of agar clonogenic growth could be identified and correlated with stage of disease, the heterogeneity in both initial clonogenic capacity and "self-renewal" capacity assessed by the ability of primary clones to propagate in liquid culture and reclone in agar was too inconsistent for the assay to be used as a prognostic index for the individual patient.

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Section: Methodsmentioning

confidence: 99%

Limitations of the clonal agar assay for the assessment of primary human ovarian tumour biopsies

Bertoncello¹,

Bradley²,

Campbell³

et al. 1982

Br J Cancer

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“…Assay of myeloid progenitors. Bone marrow and spleen cell suspensions (2,500 and 10,000 cells per dish, respectively) were assayed for the presence of macrophage lineage progenitor cells in a double-layer nutrient agar culture system exactly as previously described (5). A predetermined optimal concentration of partially purified pregnant mouse uterus extract was used as a source of macrophage CSF (6).…”

Section: Immunoprecipitations and Immunoblottingmentioning

confidence: 99%

“…Mice were immunized with the hapten nitrophenol coupled either to a CFC responsive to CSF-1 alone were assayed as an index of the committed progenitor cell compartment (5).…”

Section: Targeting Of the C-cbl Locusmentioning

confidence: 99%

Tissue Hyperplasia and Enhanced T-Cell Signalling via ZAP-70 in c-Cbl-Deficient Mice

Murphy

Schnall

Venter

et al. 1998

Molecular and Cellular Biology

348

335

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“…Hypoxia is well known to act as a stimulator of the formation and activation of cells derived from marrow precursors, including cells of the monocyte-macrophage lineage, which are closely related to osteoclasts (Bradley et al, 1978;Broxmeyer et al, 1990;Koller et al, 1992;Lewis et al, 1999). Only one early study (Stern et al, 1966) appears to have examined the effect of oxygen tension on a bone resorption parameter: the authors found that a moderate reduction in PO 2 (10%) was associated with decreased release of 3 H-proline from cultured calvaria, whereas hyperoxia (30-50%) had the opposite effect.…”

mentioning

confidence: 99%

Hypoxia is a major stimulator of osteoclast formation and bone resorption

Arnett

Gibbons

Utting

et al. 2003

Journal Cellular Physiology

262

204

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Hypoxia is known to act as a general stimulator of cells derived from marrow precursors. We investigated the effect of oxygen tension on the formation and function of osteoclasts, the cells responsible for bore resorption, which are of promonocytic origin. Using 7-and 13-day cultures of mouse marrow cells on ivory discs, we found that reducing oxygen tension from the ambient atmospheric level of 20% by increasing the proportion of nitrogen caused progressive increases in the formation of multinucleated osteoclasts and resorption pits. Peak effects occurred in 2% oxygen, where stimulations of resorption up to 21-fold were measured. Significant stimulations of osteoclast formation and resorption were observed even in severely hypoxic cultures gassed with 0.2% oxygen. Short-term cultures of cells disaggregated from rat bones indicated that hypoxia did not alter the resorptive activity of mature osteoclasts, but reduced their survival or adherence. In 3-day organ cultures of mouse calvarial bones, exposure to 2% oxygen resulted in maximal, fivefold stimulation of osteoclast-mediated calcium release, an effect equivalent to that of prostaglandin E 2 (PGE 2 ), a reference osteolytic agent. Hypoxia also caused a moderate acidosis in calvarial cultures, presumably as a result of increased anaerobic metabolism; this observation is significant because osteoclast activation is dependent on extracellular acidification. Our experiments reveal a previously-overlooked mechanism of considerable potential importance for the regulation of bone destruction. These findings may help explain the bone loss associated with a wide range of pathological states involving local or systemic hypoxia, and emphasize the importance of the vasculature in bone.

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The effect of oxygen tension on haemopoietic and fibroblast cell proliferation in vitro

Cited by 213 publications

References 13 publications

Limitations of the clonal agar assay for the assessment of primary human ovarian tumour biopsies

Limitations of the clonal agar assay for the assessment of primary human ovarian tumour biopsies

Tissue Hyperplasia and Enhanced T-Cell Signalling via ZAP-70 in c-Cbl-Deficient Mice

Hypoxia is a major stimulator of osteoclast formation and bone resorption

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