The effect of oxidized glutathione and its pharmacological analogue glutoxim on intracellular Ca2+ concentration in macrophages Ca2+

Arp2/3 complex is involved in the effect of glutoxim and molixan on intracellular Ca2+ concentration in macrophages

“…The results about suppression by compound CK0944666 of Ca 2+ entry induced by glutoxim and molixan in mac rophages agree with our data about participation of elements of cytoskeleton in regulation of Ca 2+ entry evoked by glutoxim or molixan [8] and also purinergic agonists ATP and UTP and inhibitors of endoplasmic Ca 2+ ATPases thapsigargin and cyclopiazonic acid in rat peritoneal macrophages [20]. The glutoxim or molixan evoked Ca 2+ entry into cell occurs by a store dependent mechanism [3]. Earlier [20] in the experi ments with the use of ATP, UTP, thapsigargin and cyclopiazonic acid we have shown that store depen dent Ca 2+ entry in rat peritoneal macrophages occurs according to a store dependent Ca 2+ entry model named "secretion like coupling," implying reversible translocation of the Ca 2+ store to the plasmalemma, including active participation of actin filaments [21].…”

“…Earlier we have shown for the first time that glu toxim and molixan increase the intracellular concen tration of Ca 2+ ([Ca 2+ ] i ), causing mobilization of Ca 2+ from thapsigargin sensitive Ca 2+ stores and subse quent Ca 2+ entry into rat peritoneal macrophages [2][3][4]. Using a wide range of agents affecting components of intracellular signaling systems in cells, we have shown for the first time that key players in a signal cas cade triggered by GSSG and glutoxim and leading to [Ca 2+ ] i increase in macrophages are tyrosine kinases and tyrosine phosphatases [3,5], phosphatidylinositol kinases [6], key enzymes of the phosphoinositide sig nal pathway -phospholipase C and protein kinase C [7].…”

“…Using a wide range of agents affecting components of intracellular signaling systems in cells, we have shown for the first time that key players in a signal cas cade triggered by GSSG and glutoxim and leading to [Ca 2+ ] i increase in macrophages are tyrosine kinases and tyrosine phosphatases [3,5], phosphatidylinositol kinases [6], key enzymes of the phosphoinositide sig nal pathway -phospholipase C and protein kinase C [7]. It was also found that the effect of glutoxim and molixan on [Ca 2+ ] i in macrophages was mediated by actin cytoskeleton [8] and microtubules [9].…”

The involvement of actin cytoskeleton in glutoxim and molixan effect on intracellular Ca2+-concentration in macrophages

“…Earlier we found that GSSG and glutoxim increase intracellular Ca 2+ concentration, [Ca 2+ ] i , inducing Ca 2+ mobilization from thapsigargin sensitive Ca 2+ stores and subsequent Ca 2+ entry into the rat perito neal macrophages (Kruretskaya et al, 2007a;Kurilova et al, 2008). Later we demonstrated that the same effect on [Ca 2+ ] i is observed in macrophages treated with molixan (Krutetskaya et al, 2010).…”

Glutathion as an Important Component of Thiosulfid System of Infertility Pathogenesis in Overweight Men

“…Так, установлено, что GSSG (фармакологический аналог -глутамил-цистеинил-глицин) в концентрациях, близких или превышающих те, что определяются вне клеток, про-демонстрировал способность оказывать рецептор-опосре-дованное Са 2+ -зависимое влияние на клеточные процессы при ряде заболеваний [17]. Обнаружено модулирующее влияние окисленного глутатиона на регуляцию функций цитокинов и воспалительные процессы [18].…”