The Effect of Oxidative Stress on the Chicken Ovary: Involvement of Microbiota and Melatonin Interventions

Abstract

The poultry ovary is used as a classic model to study ovarian biology and ovarian cancer. Stress factors induced oxidative stress to cause follicle atresia, which may be a fundamental reason for the reduction in fertility in older laying hens or in aging women. In the present study, we set out to characterize the relationships between oxidative stress and ovarian function. Layers (62 weeks of age; BW = 1.42 ± 0.12 kg) were injected with tert-butyl hydroperoxide (tBHP) at 0 (CON) and 800 μmol/kg BW (oxidative stress group, OS) for 24 days and the role of melatonin (Mel) on tBHP-induced ovary oxidative stress was assessed through ovary culture in vitro. The OS (800 μmol/kg BW tert-butyl hydroperoxide) treatment decreased the reproduction performance and ovarian follicle numbers. OS decreased the expression of SIRT1 and increased the P53 and FoxO1 expression of the ovary. A decreased Firmicutes to Bacteroidetes ratio, enriched Marinifilaceae (family), Odoribacter (genus) and Bacteroides_plebeius (species) were observed in the cecum of the OS group. Using Mel in vitro enhanced the follicle numbers and decreased the ovary cell apoptosis induced by tBHP. In addition, it increased the expression of SIRT1 and decreased the P53 and FoxO1 expression. These findings indicated that oxidative stress could decrease the laying performance, ovarian function and influence gut microbiota and body metabolites in the layer model, while the melatonin exerts an amelioration the ovary oxidative stress through SIRT1-P53/FoxO1 pathway.

Dietary Vitamin A Improved the Flesh Quality of Grass Carp (Ctenopharyngodon idella) in Relation to the Enhanced Antioxidant Capacity through Nrf2/Keap 1a Signaling Pathway

“…Lipid and protein oxidation, not only is one of the major causes of meat quality deterioration [ 12 ], but it also leads to damage of the structural integrity of cells [ 38 ]. High unsaturated lipid levels in fish flesh make it more susceptible to oxidative deterioration, which is mainly caused by an imbalance between ROS production and antioxidative defense [ 12 ].…”

Dietary resveratrol improved production performance, egg quality, and intestinal health of laying hens under oxidative stress

“…A total of 40 Lohmann gray hens (25 wk of age; initial BW = 1.44 ± 0.10 kg) with similar performance, were randomly assigned to four treatments with 10 replicates of 1 hen. All laying hens were randomly allotted into a 2 by 2 factorial design that were dietary supplementation either resveratrol (0 or 600 mg/kg) or intraperitoneal injection of tert-butyl hydroperoxide ( tBHP ) (0 or 800 μmol/kg BW) for 28 d (The dosage of tBHP were set according to the previous study; Wang et al., 2021 ). Resveratrol (≥99%) and tBHP (≥99%) were purchased from Sigma-Aldrich Corp (St Louis, MO).…”

“…Moreover, it has been proved that oxidative stress plays a role in follicular atresia to lead to ovarian aging and reproductive disorders ( Gupta et al., 2006 ; Devine et al., 2012 ; Shen et al., 2012 ). Serval previous studies has shown that oxidative stress could led to reduction in production performance and intestinal health ( Mishra and Jha, 2019 ; Wang et al., 2019 , 2021 ; Li et al., 2020 , Wang et al., 2021 ).…”

“…Oxidative stress has been proven to be linked to internal mechanism for many environmental stressors (i.e., UV, ionizing radiations, pollutants, and heat stress), xenobiotics (mycotoxins, heavy metals, pesticides, etc. ), health disorders and aging ( Pizzino et al., 2017 ; Liguori et al., 2018 ; Wang et al., 2019 , 2021 ; Liu et al., 2021a , b , Wang et al., 2021 ). Moreover, it has been proved that oxidative stress plays a role in follicular atresia to lead to ovarian aging and reproductive disorders ( Gupta et al., 2006 ; Devine et al., 2012 ; Shen et al., 2012 ).…”

“…Resveratrol (trans-3, 5, 40-trihydroxytilbene; RV ) is a naturally polyphenol compound found in berries, nuts, skins of grapes and thus in red wine ( Jang et al., 1997 ). RV has antioxidant, antitumor, antiviral, and free radical scavenging properties, that is thought to be an effective compound for the prevention aging and age-related diseases ( Li et al., 2018 ; Nunes et al., 2018 ; Zhou et al., 2021 , Wang et al., 2021 ). Also, RV is associated with protection against oxidative stress of ovary to increase follicle numbers and prolong the ovary life-extending effect in human and rat model ( Liu et al., 2013 ; Li and Liu, 2018 ; Ochiai and Kuroda, 2019 ).…”