The effect of oxidative stress on the membrane dipole potential of human red blood cells

Jewell, S. A.; Petrov, Peter G.; Winlove, C. Peter

doi:10.1016/j.bbamem.2012.12.019

Cited by 31 publications

(32 citation statements)

References 37 publications

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“…Previous studies 28 have demonstrated that human red blood cell membranes can be readily labelled with Di-8-ANEPPS. Ratiometric imaging of healthy discotic RBCs indicates that is of the order of 250 mV, varying by around 30 mV along the surface of the cell.…”

Section: Ratiometric Imaging Of Di-8-anepps Labelled Red Blood Cells mentioning

confidence: 99%

“…The value of differs by 14 % between the two regions of the post-toxin vesicle. This is likely to arise from the change in surface morphology and increase in local curvature in the membrane 28 around the DAG droplet which enables a change in lipid packing as well as changes in spatial organisation of lipid molecules with respect to each other, lipid headgroup configuration and headgroup-associated water.…”

Section: Ratiometric Imaging Of Di-8-anepps-labelled Vesicles Shows Amentioning

confidence: 99%

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Clostridium perfringens α-toxin interaction with red cells and model membranes

et al. 2015

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The effects of Clostridium perfringens -toxin on host cells have previously been studied extensively but the biophysical processes associated with toxicity are poorly understood. The work reported here shows that the initial interaction between the toxin and lipid membrane leads to measurable changes in the physical properties and morphology of the membrane. A Langmuir monolayer technique was used to assess the response of different lipid species to toxin. Sphingomyelin and unsaturated phosphatidylcholine showed the highest susceptibility to toxin lypolitic action, with a two stage response to the toxin (an initial, rapid hydrolysis stage followed by the insertion and/or reorganisation of material in the monolayer). Fluorescence confocal microscopy on unsaturated phosphatidylcholine vesicles shows that the toxin initially aggregates at discrete sites followed by the formation of localised "droplets" accumulating the hydrolysis products. This process is accompanied by local increases in the membrane dipole potential by about 50 (± 30) mV. In contrast, red blood cells incubated with the toxin suffered a decrease of the membrane dipole potential by 50 (± 40) mV in areas of high toxin activity (equivalent to a change in electric field strength of 10 7 Vm -1) which is sufficient to affect the functioning of the cell membrane. Changes in erythrocyte morphology caused by the toxin are presented, and the early stages of interaction between toxin and membrane are characterised using thermal shape fluctuation analysis of red cells which revealed two distinct regimes of membrane-toxin interaction.

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Section: Ratiometric Imaging Of Di-8-anepps Labelled Red Blood Cells mentioning

confidence: 99%

Section: Ratiometric Imaging Of Di-8-anepps-labelled Vesicles Shows Amentioning

confidence: 99%

Clostridium perfringens α-toxin interaction with red cells and model membranes

et al. 2015

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“…The conformation and properties of Hb were studied by Raman scattering (RS) using inVia confocal Raman microscope (Renishaw, Wotton-under-Edge, UK) [21][22][23][24] with short focus high luminosity monochromator (focal length not more than 250 mm). For excitation of Raman spectra, a laser was used (wavelength of 532 nm, maximum power of 100 mW and 100£ objective).…”

Section: Conformation and Properties Of Hbmentioning

confidence: 99%

Effect of hypoxia on the composition and state of lipids and oxygen-transport properties of erythrocyte haemoglobin

Ревин

Grunyushkin

Gromova

et al. 2016

Biotechnology & Biotechnological Equipment

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The aim of this study was to investigate the relationship between the composition and state of erythrocytic lipids and oxygen-binding properties of erythrocyte haemoglobin-derived haematoporphyrin under hypoxia. The results from this study showed that hypoxia causes a profound change in the composition of the lipids that form the bilayer of erythrocyte membranes, as well as in the functional characteristics of erythrocytes. The detected changes correlate with morphological reconstruction of erythrocytes and, consequently, affect the oxygen-transport properties of erythrocytes. The obtained results showed that the lipid phase plays an important role in the functioning of erythrocytes in health as well as in event of development of ischemic heart disease and other pathological processes that accompany hypoxia.

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“…5). We have previously shown that treatment of RBCs with cumOOH increased both the membrane bending elastic modulus (64) and membrane dipole potential (77), yet these cells are much more resistant to toxin compared with healthy cells, for which higher bending rigidity and dipole potential meant lower resistance to PLY (Fig. 8, A and B, respectively).…”

Section: Discussionmentioning

confidence: 99%

“…This motivated us to investigate whether differences in the membrane physical properties of individual cells within a population of RBCs are correlated with their susceptibility to PLY. We measured the membrane bending elastic modulus (a primary measure of the mechanical compliance of the lipid bilayer (75)), the membrane electrostatic dipole potential (76,77), and the membrane electrostatic surface potential (60, 61) for a number of individual cells within a population of RBCs before toxin exposure. The buffer surrounding the cells was then exchanged with buffer containing toxin, and the time to lysis, i.e.…”

Section: Differences In the Membrane Physical Properties Of Individuamentioning

confidence: 99%

Red Blood Cell Susceptibility to Pneumolysin

Bokori-Brown

Petrov

Khafaji

et al. 2016

Journal of Biological Chemistry

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This study investigated the effect of the biochemical and biophysical properties of the plasma membrane as well as membrane morphology on the susceptibility of human red blood cells to the cholesterol-dependent cytolysin pneumolysin, a key virulence factor of Streptococcus pneumoniae, using single cell studies. We show a correlation between the physical properties of the membrane (bending rigidity and surface and dipole electrostatic potentials) and the susceptibility of red blood cells to pneumolysin-induced hemolysis. We demonstrate that biochemical modifications of the membrane induced by oxidative stress, lipid scrambling, and artificial cell aging modulate the cell response to the toxin. We provide evidence that the diversity of response to pneumolysin in diabetic red blood cells correlates with levels of glycated hemoglobin and that the mechanical properties of the red blood cell plasma membrane are altered in diabetes. Finally, we show that diabetic red blood cells are more resistant to pneumolysin and the related toxin perfringolysin O relative to healthy red blood cells. Taken together, these studies indicate that the diversity of cell response to pneumolysin within a population of human red blood cells is influenced by the biophysical and biochemical status of the plasma membrane and the chemical and/or oxidative stress pre-history of the cell.The interaction of toxins with their target cells is generally characterized by a dose-response curve that is sigmoidal in shape (1). This shape is taken to reflect the differing susceptibilities of individual cells within the population. The fact that red blood cells (RBCs), with their less developed glycocalyx compared with other cell types (2), exhibit such a response is evidence that the initial interaction between the toxin and the plasma membrane is subject to, and probably the main determinant of, such variability. Therefore, it is important to understand the biochemical and biophysical factors affecting toxin-membrane interactions and the resulting variability of susceptibility within the same cell population.Individual RBCs within a population differ significantly (3) with respect to their shape, volume, and surface area. Some of these changes are related to cell age, due to the variety of mechanical and chemical stresses that an RBC undergoes in its life span of ϳ120 days (4, 5), and others can be associated with diseases, such as diabetes and with acute conditions, such as sepsis, among many others (6), and thus are likely to be present in the membranes of many other cell types.Previous work on bulk cell preparations revealed that in addition to its normal discocytic shape, the RBC at rest can assume a variety of other distinct shapes, such as echinocytes and acanthocytes, characterized by exterior projections, and stomatocytes with cup-shaped invaginations (7). Many of these unusual shapes appear in normal blood at a frequency of about 1%. However, during blood bank storage and in many inflammatory, degenerative, and microvascular disorders, the frequ...

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The effect of oxidative stress on the membrane dipole potential of human red blood cells

Cited by 31 publications

References 37 publications

Clostridium perfringens α-toxin interaction with red cells and model membranes

Clostridium perfringens α-toxin interaction with red cells and model membranes

Effect of hypoxia on the composition and state of lipids and oxygen-transport properties of erythrocyte haemoglobin

Red Blood Cell Susceptibility to Pneumolysin

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