The effect of overexpression of Dlx2 on the migration, proliferation and osteogenic differentiation of cranial neural crest stem cells

Dai, Jiewen; Kuang, Ying; Fang, Bing; Gong, Hui; Shen, Lu; Mou, Zhifang; Sun, Hao; Dong, Yuefu; Lu, Jingting; Zhang, Wenbin; Zhang, Jianfei; Wang, Zhugang; Wang, Xudong; Shen, Guofang

doi:10.1016/j.biomaterials.2012.11.051

Cited by 21 publications

(31 citation statements)

References 64 publications

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“…1 Genetic factors and tobacco chewing also play an important role. 1,2,4,6,7,10,11 These factors work together additively or synergistically.…”

Section: Discussionmentioning

confidence: 97%

“…1 The effect of opium consumption on inducing some cancers, such as laryngeal or esophageal cancer, has been assessed by some researchers. 11,12 The development of oral cancer in opium users is a notable theory. 13 Opium addiction is a concerning issue in the Iranian society.…”

Section: Discussionmentioning

confidence: 99%

“…The cells were then detached with 0.25% of trypsin/ethylenediamine tetraacetic acid (EDTA), subcultured at a density of 1 Â 10 5 cells/cm 2 in 100-mm dishes, and cultured as previously described. 11 Briefly, the pooled cells were plated on one 10-cm dish in DMEM medium containing 10% of FBS and 1% of penicillinstreptomycin at 37°C in 5% of carbon dioxide for 5 to 7 days. After reaching confluence, the CSCs were continuously passaged for further experiments.…”

Section: Culture Of Cscsmentioning

confidence: 99%

“…11 Briefly, total cellular RNA was extracted from cell samples using the TRIzol reagent (Invitrogen) according to the manufacturer's instructions. The quality and quantity of the total RNA were analyzed using a NanoDrop 1000 spectrophotometer (Thermo Scientific) following the recommended protocol.…”

Section: Quantitative Real-time Polymerase Chain Reactionmentioning

confidence: 99%

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The Effect of Fibroblast Growth Factor 9 on the Osteogenic Differentiation of Calvaria-Derived Mesenchymal Cells

Lu¹,

Dai²,

Wang³

et al. 2014

Journal of Craniofacial Surgery

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Fibroblast growth factor 9 (FGF9) plays complicated and crucial roles in bone formation, and the biologic effect of FGF9 may depend on the gene dosage, developmental stage, cell type, or interactions with other cytokines. In this study, we demonstrated that FGF9 enhanced the phosphorylation of extracellular regulated protein kinases 1/2 in calvaria-derived mesenchymal cells. However, the inhibitory effect of FGF9 on the osteogenic differentiation of calvaria-derived mesenchymal cells did not depend on the phosphorylation of extracellular regulated protein kinases 1/2. Combined with the previous findings that FGF9 promotes dental pulp stem cells chondrogenesis in vitro, we suggest that FGF9 may be applied to promote chondrogenesis and inhibit osteogenesis in mesenchymal stem cells in vitro.

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“…1 Genetic factors and tobacco chewing also play an important role. 1,2,4,6,7,10,11 These factors work together additively or synergistically.…”

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Culture Of Cscsmentioning

confidence: 99%

Section: Quantitative Real-time Polymerase Chain Reactionmentioning

confidence: 99%

See 2 more Smart Citations

The Effect of Fibroblast Growth Factor 9 on the Osteogenic Differentiation of Calvaria-Derived Mesenchymal Cells

Lu¹,

Dai²,

Wang³

et al. 2014

Journal of Craniofacial Surgery

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“…Both are regulators of cNC-derived mesenchyme survival (Macatee et al, 2003; Dai et al, 2013). This may occur through miR-452, which is highly expressed in cNC-derived mesenchyme at E10.5 in the first pharyngeal arch and regulates Fgf8 and Dlx2 expression.…”

Section: Introductionmentioning

confidence: 99%

MicroRNAs in Palatogenesis and Cleft Palate

et al. 2017

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Palatogenesis requires a precise spatiotemporal regulation of gene expression, which is controlled by an intricate network of transcription factors and their corresponding DNA motifs. Even minor perturbations of this network may cause cleft palate, the most common congenital craniofacial defect in humans. MicroRNAs (miRNAs), a class of small regulatory non-coding RNAs, have elicited strong interest as key regulators of embryological development, and as etiological factors in disease. MiRNAs function as post-transcriptional repressors of gene expression and are therefore able to fine-tune gene regulatory networks. Several miRNAs are already identified to be involved in congenital diseases. Recent evidence from research in zebrafish and mice indicates that miRNAs are key factors in both normal palatogenesis and cleft palate formation. Here, we provide an overview of recently identified molecular mechanisms underlying palatogenesis involving specific miRNAs, and discuss how dysregulation of these miRNAs may result in cleft palate.

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Hypoxia and hyperoxia differentially control proliferation of rat neural crest stem cells via distinct regulatory pathways of the HIF1α–CXCR4 and TP53–TPM1 proteins

Chen

Hsia

et al. 2017

Developmental Dynamics

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The effect of overexpression of Dlx2 on the migration, proliferation and osteogenic differentiation of cranial neural crest stem cells

Cited by 21 publications

References 64 publications

The Effect of Fibroblast Growth Factor 9 on the Osteogenic Differentiation of Calvaria-Derived Mesenchymal Cells

The Effect of Fibroblast Growth Factor 9 on the Osteogenic Differentiation of Calvaria-Derived Mesenchymal Cells

MicroRNAs in Palatogenesis and Cleft Palate

Hypoxia and hyperoxia differentially control proliferation of rat neural crest stem cells via distinct regulatory pathways of the HIF1α–CXCR4 and TP53–TPM1 proteins

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