The effect of ovarian steroids on oxytocin-stimulated secretion and synthesis of prostaglandins in bovine myometrial cells

Słonina, Dominika; Kowalik, Magdalena K.; Subocz, Mariola; Kotwica, J.

doi:10.1016/j.prostaglandins.2009.08.006

Cited by 20 publications

(12 citation statements)

References 31 publications

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“…Therefore, it is possible that a PGRMC1/ SERBP1 complex is involved in nongenomic P4 action and can play a role in the maintenance of pregnancy in cows. It has been suggested that P4 impairs binding of OT to its membrane receptor via a nongenomic mechanism [9,13,14] and thus diminishes levels of intracellular Ca 2+ and decreases prostaglandin secretion from the bovine endometrium and myometrium [10,12]. However, the nongenomic effect of P4 on the OT receptor was not confirmed by other authors [31,32].…”

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confidence: 96%

“…58, No 3, 2012 EFFECT OF PROGESTERONE ON THE BOVINE MYOMETRIUM 289 both genomic and nongenomic pathways [12]. So, it can be assumed that the latter effect of P4 was evoked partly via a membrane progesterone receptor, PGRMC1, found in the human [20] and mouse myometrium [22].…”

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confidence: 99%

“…This nongenomic effect of P4 has been found in a number of tissues from the female reproductive tract [5,7,8] in mammals, including cows [9][10][11][12], but the nature of this mechanism is not fully understood. It has been suggested that P4, a lipophilic substance, can modify the fluidity of the cell membrane and thus change the affinity of membrane receptors for their ligands [9,13,14].…”

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confidence: 99%

“…We have shown that P4 decreased intracellular Ca 2+ mobilization in bovine myometrial cells [21] and that P4 inhibited oxytocin (OT)-stimulated PGF2α and PGE2 secretion from these cells by both genomic and nongenomic pathways [12]. So, it can be assumed that the latter effect of P4 was evoked partly via a membrane progesterone receptor, PGRMC1, found in the human [20] and mouse myometrium [22].…”

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confidence: 99%

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Expression of Progesterone Receptor Membrane Component 1, Serpine mRNA Binding Protein 1 and Nuclear Progesterone Receptor Isoforms A and B in the Bovine Myometrium During the Estrous Cycle and Early Pregnancy

Słonina

Kowalik

Kotwica

2012

Journal of Reproduction and Development

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Abstract. The aim of this study was to investigate the (1) expression of progesterone membrane component 1 (PGRMC1), serpine mRNA binding protein 1 (SERBP1) and progesterone receptor (PR) mRNA and (2) protein expression levels of PGRMC1, SERBP1 and PR isoforms A and B in the bovine myometrium during the estrous cycle and early pregnancy. Uteri from cows on days 1-5, 6-10, 11-16 and 17-21 of the estrous cycle and weeks 3-5, 6-8 and 9-12 of pregnancy were used (n=5-6 per period). There were no changes (P>0.05) in PGRMC1 mRNA expression during the estrous cycle, while expression of SERBP1 and PR mRNA was the lowest (P<0.05) on days 11-16 relative to other days of the cycle. The highest mRNA expression of PGRMC1, SERBP1 and PR was found during pregnancy. There were no changes (P>0.05) in SERBP1 protein expression in cycling and pregnant cows, while the highest (P<0.05) PGRMC1 protein expression was found during weeks 3-5 of pregnancy. Similar protein expression profiles for PRA and PRB were found, and protein levels were highest on days 1-5 of the estrous cycle. From day 6 of the cycle, PRA and PRB protein expression decreased and were maintained at this lower level during pregnancy. In conclusion, our study assessed mRNA and protein expression levels of PGRMC1, SERBP1 and PR in the bovine myometrium during the estrous cycle and the first trimester of pregnancy. It is possible that progesterone (P4) affects myometrial function in a genomic and nongenomic manner. P rogesterone (P4), which is produced in the corpus luteum (CL), regulates various female reproductive functions. This hormone is also responsible for morphological, functional and structural changes in the endometrium during the luteal phase and for suppression of myometrial contractions, which prepares the uterus for blastocyst implantation and ensures maintenance of pregnancy [1]. Many functions of P4 are mediated through binding to its specific nuclear progesterone receptor (nPR), which acts as a ligand-transcription factor and is expressed in two main isoforms, A (PRA) and B (PRB) [2]. PRA (94 kDa) is about 164 amino acids shorter than PRB (120 kDa) in humans [2], and a similar molecular mass was found in cattle [3,4]. The different isoforms of nPR play different roles in the cells. Isoform B acts mainly as an activator of progesterone-responsive genes, while PRA can inhibit the activity of PRB and other nuclear receptors such as the estrogen, glucocorticoid and mineralocorticoid receptors [2].P4 can also exert its effects more directly, by rapid, nongenomic pathways [5][6][7]. This nongenomic effect of P4 has been found in a number of tissues from the female reproductive tract [5,7,8] in mammals, including cows [9][10][11][12], but the nature of this mechanism is not fully understood. It has been suggested that P4, a lipophilic substance, can modify the fluidity of the cell membrane and thus change the affinity of membrane receptors for their ligands [9,13,14]. Moreover, P4 can be bound by its specific membrane receptor to stimulate early intracellular...

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confidence: 96%

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confidence: 99%

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confidence: 99%

mentioning

confidence: 99%

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Expression of Progesterone Receptor Membrane Component 1, Serpine mRNA Binding Protein 1 and Nuclear Progesterone Receptor Isoforms A and B in the Bovine Myometrium During the Estrous Cycle and Early Pregnancy

Słonina

Kowalik

Kotwica

2012

Journal of Reproduction and Development

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“…Entretanto, dentre as sintases de PGE2, os esteroides foram capazes de modular somente a expressão gênica da isoforma PTGES1, apresentando maior expressão no grupo F/CLG (32%; P>0,10; Tabela 2). Tais resultados contrastaram com os obtidos por pesquisas envolvendo cultivo de células miometriais por 14 a 16 dias, onde a suplementação com esteroides não alterou a expressão de PTGES1 (SLONINA et al, 2009). Adicionalmente, em experimento retrospectivo, as enzimas PTGES1 e PTGES2 não apresentaram expressão gênica diferenciada entre grupos de vacas apresentando endométrio associado à alta ou baixa receptividade, tanto em análises de microarranjo como em qPCR (PONSUKSILI et al, 2012).…”

Section: Abundância De Rnam De Fosfolipases (Pla2) E Ciclooxigenasesunclassified

Efeito do ambiente endócrino periovulatório na expressão gênica do endométrio durante a primeira semana do ciclo estral em bovinos: biossíntese e sinalização de eicosanoides

Oliveira¹

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AGRADECIMENTOSPrimeiramente a Deus por sempre estar guiando cada passo que dou em minha vida, além da proteção, saúde e força de vontade.Principalmente por sempre colocar anjos em minha vida, me presenteando com uma família linda e grandes amigos.À minha mãe Maria Helena, ao meu pai Edmilson e ao meu irmão Éverton pelos conselhos, incentivos e esforços sem medida, sempre oferecendo o máximo que podem para que consiga alcançar todos os meus objetivos. E pelo amor, carinho e proteção em qualquer situação, a qualquer hora e lugar. Tudo que eu faço é sempre pensando em ser motivo de orgulho para vocês.Ao Prof. Mário Binelli por oferecer além do necessário ao desenvolvimento deste trabalho, sempre pensando no crescimento dos seus pós-graduandos. Pela disponibilidade, paciência, conselhos e orientações. Por me fazer despertar o interesse pela pesquisa.Obrigada pelas oportunidades e confiança.A Cláudia Maria Bertan por toda atenção, confiança e por ter me inspirado a gostar das atividades desenvolvidas no LFEM. A Juliane Diniz, Éverton, Roney, Moana e Mariana, pela grande ajuda na edição, revisões e força nos momentos finais deste trabalho, agradecimentos sempre serão poucos perto de toda ajuda de vocês.Agradeço a todos os meus amigos e familiares que perto ou longe sempre se fazem presentes em minha vida, dando apoio e incentivo para a conquista de meus sonhos. i.m.) on D0. Plasma was obtained for measurement of estradiol concentrations from D-2 to D0 and from D0 to D7 for progesterone measurements. On D7, the animals that ovulated in response to GnRH were slaughtered and uterus flushing and endometrium were collected. Differences between group means were determined by student′s t test. Animals from LF/CL group presented larger preovulatory follicles, corpus luteum, estradiol and progesterone levels when compared to animals from SF/CL group (P<0.1). The quantification of the transcripts was performed by qPCR.PTGES, AKR1C3, AKR1C4, CBR1, PTGIS and ALOX12 were more abundant and PLA2G10 was less expressed in the endometrium from animals from LF/CL when compared to SF/CL (P<0.10). Metabolites concentration was measured by mass spectrometry in endometrial tissue (pg/g) and uterine flushings (ng/mL). The metabolite 9-HETE was more abundant on uterine flushings from animals with SF/CL (P<0.05). Different concentrations of estradiol (proestrous) and progesterone (beginning of diestrous) modulated the expression of genes related to eicosanoids synthesis, but it did not affect eicosanoids levels in the endometrium and uterine flushing in beef cattle on day 7 of the estrous cycle.

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Steroid Hormone Receptors in the Corpus Luteum

Rękawiecki

Kowalik

Kotwica

2016

The Life Cycle of the Corpus Luteum

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The effect of ovarian steroids on oxytocin-stimulated secretion and synthesis of prostaglandins in bovine myometrial cells

Cited by 20 publications

References 31 publications

Expression of Progesterone Receptor Membrane Component 1, Serpine mRNA Binding Protein 1 and Nuclear Progesterone Receptor Isoforms A and B in the Bovine Myometrium During the Estrous Cycle and Early Pregnancy

Expression of Progesterone Receptor Membrane Component 1, Serpine mRNA Binding Protein 1 and Nuclear Progesterone Receptor Isoforms A and B in the Bovine Myometrium During the Estrous Cycle and Early Pregnancy

Efeito do ambiente endócrino periovulatório na expressão gênica do endométrio durante a primeira semana do ciclo estral em bovinos: biossíntese e sinalização de eicosanoides

Steroid Hormone Receptors in the Corpus Luteum

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