The Effect of Ovarian Extracts on the Calcium Blood‐level in Man

Abstract

IN a previous communication (1) we showed that alcoholic extracts of the different portions of the ovary when injected into rabbits produce a fall of blood-calcium. We then tried the effects of this extract on man.Our procedure was in all respects the same as that used for rabbits, as described in our previous paper. We took the additional precaution of purifying our extracts and removing all traces of cholesterol until the preparation no longer gave the Liebermann-Burchardt reaction for cholesterol. The liquor folliculi and residual ovary were derived from cows' and pigs' ovaries, and the corpus luteum from cows' only. The extracts were sterilised by heating to 800 C. for about half an hour, and were then dissolved in olive oil which had been sterilised by boiling.

A Study of Calcium Metabolism in the Human Female

“…The year following this work Mirvish and Bosman (8) correlated the blood ealcium content with the secretion of the ovarian hormone, and then demonstrated that the introduction of foUicular extract (prepared according to the method of Allen and Doisy (10) into rabbits reduced the blood calcium. The same result was also found wheu the extract was injected into humans (9), either male or female.…”