The effect of osmotic pressure on the membrane fluidity of Saccharomyces cerevisiae at different physiological temperatures

Laroche, Céline; Beney, Laurent; Marechal, Pierre-André; Gervais, Patrick

doi:10.1007/s002530000583

Cited by 106 publications

(76 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Membrane fluidity is believed to play a crucial role in stress resistance, and the relationship between fluidity and stress resistance has been used to predict the outcome of cell resistance to stress (15,24,39). The membrane composition of yeast during stress has been extensively studied, and it was reported that more unsaturation in the membrane increased the sensitivity to heat (40).…”

Section: Discussionmentioning

confidence: 99%

Modification of the Technical Properties of Lactobacillus johnsonii NCC 533 by Supplementing the Growth Medium with Unsaturated Fatty Acids

Müller

Ross

Sybesma

et al. 2011

Appl Environ Microbiol

View full text Add to dashboard Cite

The aim of this study was to investigate the influence of supplementing growth medium with unsaturated fatty acids on the technical properties of the probiotic strain Lactobacillus johnsonii NCC 533, such as heat and acid tolerance, and inhibition of Salmonella enterica serovar Typhimurium infection. Our results showed that the membrane composition and morphology of L. johnsonii NCC 533 were significantly changed by supplementing a minimal Lactobacillus medium with oleic, linoleic, and linolenic acids. The ratio of saturated to unsaturated plus cyclic fatty acids in the bacterial membrane decreased by almost 2-fold when minimal medium was supplemented with unsaturated fatty acids (10 g/ml). The subsequent acid and heat tolerance of L. johnsonii decreased by 6-and 20-fold when the strain was grown in the presence of linoleic and linolenic acids, respectively, compared with growth in oleic acid (all at 10 g/ml). Following acid exposure, significantly higher (P < 0.05) oleic acid content was detected in the membrane when growth medium was supplemented with linoleic or linolenic acid, indicating that saturation of the membrane fatty acids occurred during acid stress. Cell integrity was determined in real time during stressed conditions using a fluorescent viability kit in combination with flow cytometric analysis. Following heat shock (at 62.5°C for 5 min), L. johnsonii was unable to form colonies; however, 60% of the bacteria showed no cell integrity loss, which could indicate that the elevated heat inactivated vital processes within the cell, rendering it incapable of replication. Furthermore, L. johnsonii grown in fatty acid-enriched minimal medium had different adhesion properties and caused a 2-fold decrease in S. enterica serovar Typhimurium UK1-lux invasion of HT-29 epithelial cells compared with bacteria grown in minimal medium alone. This could be related to changes in the hydrophobicity and fluidity of the membrane. Our study shows that technical properties underlying probiotic survivability can be affected by nutrient composition of the growth medium.

show abstract

Section: Discussionmentioning

confidence: 99%

Modification of the Technical Properties of Lactobacillus johnsonii NCC 533 by Supplementing the Growth Medium with Unsaturated Fatty Acids

Müller

Ross

Sybesma

et al. 2011

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…In development of HVA, Sinensky (Sinensky, 1974) observed using Electron Spin Resonance (ESR) that the order parameter of E. coli membranes remained constant, determined as a function of spin probe rotational correlation time, despite their phospholipid compositions changing in response to different growth temperatures. Subsequently, evidence for HVA as a conserved evolutionary mechanism emerged (Behan-Martin et al, 1993;Cossins and Prosser, 1978) and HVA has since been reported under many other changing environmental conditions, such as in response to hydrostatic pressure (Behan et al, 1992;Macdonald, 1986, 1984), osmotic pressure (Laroche et al, 2001) , low magnetic field strength (Santoro et al, 1997) and chemicals such as PCB-153 (Gonzalez et al, 2013), mitelfosine (Rybczynska et al, 2001) and crude oil (Mazzella et al, 2005). To date evidence for HVA has been found in species across the three domains of life and it is arguably the most widely used theory to explain phospholipid compositional changes, usually through the concept of maintaining membrane fluidity or membrane viscosity.…”

Section: Introductionmentioning

confidence: 99%

Mammalian phospholipid homeostasis: Homeoviscous adaptation deconstructed by lipidomic data driven modelling

Dymond

2015

Chemistry and Physics of Lipids

View full text Add to dashboard Cite

One of the mostly widely cited theories of phospholipid homeostasis is the theory of homeoviscous adaptation (HVA). HVA states that cells maintain membrane order (frequently discussed in terms of membrane fluidity or viscosity) within tight conditions in response to environmental induced changes in membrane lipid composition. In this article we use data driven modelling to investigate membrane order, using methodology we previously developed to investigate another theory of phospholipid homeostasis, the intrinsic curvature hypothesis. A set of coarse-grain parameters emerge from our model which can be used to deconstruct the relative contribution of each component membrane phospholipid to net membrane order. Our results suggest, for the membranes in the mammalian cells we have studied, that a ratio control function can be used to model membrane order. Using asynchronous cell lines we quantify the relative contribution of around 130 lipid species to net membrane order, finding that around 16 of these phospholipid species have the greatest effect in vivo. Then using lipidomic data obtained from partially synchronised cultures of HeLa cells we are able to demonstrate that these same 16 lipid species drive the changes in membrane order observed around the cell cycle.Our findings in this study suggest, when compared with our previous work, that cells maintain both membrane order and membrane intrinsic curvature within tight conditions.

show abstract

“…For example, reduced incubation temperatures increase the molecular order of membrane lipids, i.e., rigidification (29), and alter the activity of membrane-associated enzymes and transporters (5). Other stresses, e.g., heat shock, freezing, and osmotic stress, induce dramatic changes in the organization and dynamic properties of membrane lipids (12,27). To date, most of the research in this field has focused on the connections between the physical state of the membrane and cold tolerance.…”

mentioning

confidence: 99%

Fluidization of Membrane Lipids Enhances the Tolerance of Saccharomyces cerevisiae to Freezing and Salt Stress

Rodríguez-Vargas

Sánchez‐García

Martínez-Rivas

et al. 2007

Appl Environ Microbiol

185

101

View full text Add to dashboard Cite

Unsaturated fatty acids play an essential role in the biophysical characteristics of cell membranes and determine the proper function of membrane-attached proteins. Thus, the ability of cells to alter the degree of unsaturation in their membranes is an important factor in cellular acclimatization to environmental conditions. Many eukaryotic organisms can synthesize dienoic fatty acids, but Saccharomyces cerevisiae can introduce only a single double bond at the ⌬ 9 position. We expressed two sunflower (Helianthus annuus) oleate ⌬ 12 desaturases encoded by FAD2-1 and FAD2-3 in yeast cells of the wild-type W303-1A strain (trp1) and analyzed their effects on growth and stress tolerance. Production of the heterologous desaturases increased the content of dienoic fatty acids, especially 18:2⌬ 9,12 , the unsaturation index, and the fluidity of the yeast membrane. The total fatty acid content remained constant, and the level of monounsaturated fatty acids decreased. Growth at 15°C was reduced in the FAD2 strains, probably due to tryptophan auxotrophy, since the trp1 (TRP1) transformants that produced the sunflower desaturases grew as well as the control strain did. Our results suggest that changes in the fluidity of the lipid bilayer affect tryptophan uptake and/or the correct targeting of tryptophan transporters. The expression of the sunflower desaturases, in either Trp ؉ or Trp ؊ strains, increased NaCl tolerance. Production of dienoic fatty acids increased the tolerance to freezing of wild-type cells preincubated at 30°C or 15°C. Thus, membrane fluidity is an essential determinant of stress resistance in S. cerevisiae, and engineering of membrane lipids has the potential to be a useful tool of increasing the tolerance to freezing in industrial strains.Tolerance to freezing is an essential trait influencing the viability and leavening capacity of baker's yeast in frozen dough (4, 42). The so-called frozen-dough technology has been widely accepted by consumers and bakers due to several advantages, which include supplying oven-fresh bakery products and improving labor conditions. However, no appropriate industrial strain with a high tolerance to freezing is available, and it is unlikely that the classical breeding program could significantly improve this trait. Freezing is a complex and multifaceted stress, in which different stressors and stress responses appear to play important roles. Cells exposed to subzero temperatures are injured by the formation of ice crystals and crystal growth during frozen storage (33). At a slow freezing rate, cells are exposed to hyperosmotic solutions and equilibrate by movement of water across the membranes (65). Finally, during the thawing process, cells can suffer biochemical damage by oxidative stress (16). It is not surprising, therefore, that tolerance to freezing involves several mechanisms working in concert.Biological membranes are the first barrier that separates cells from their environment and are a primary target for damage during environmental stress. Sudden changes in envi...

show abstract

The effect of osmotic pressure on the membrane fluidity of Saccharomyces cerevisiae at different physiological temperatures

Cited by 106 publications

References 28 publications

Modification of the Technical Properties of Lactobacillus johnsonii NCC 533 by Supplementing the Growth Medium with Unsaturated Fatty Acids

Modification of the Technical Properties of Lactobacillus johnsonii NCC 533 by Supplementing the Growth Medium with Unsaturated Fatty Acids

Mammalian phospholipid homeostasis: Homeoviscous adaptation deconstructed by lipidomic data driven modelling

Fluidization of Membrane Lipids Enhances the Tolerance of Saccharomyces cerevisiae to Freezing and Salt Stress

Contact Info

Product

Resources

About