The effect of orexin B on steroidogenic acute regulatory protein, P450 side-chain cleavage enzyme, and 3β-hydroxysteroid dehydrogenase gene expression, and progesterone and androstenedione secretion by the porcine uterus during early pregnancy and the estrous cycle1

Kisielewska, Katarzyna; Rytelewska, Edyta; Gudelska, Marlena; Kieżun, Marta; Dobrzyń, Kamil; Szeszko, Karol; Bors, Kinga; Wyrębek, Joanna; Kamiński, Tadeusz

doi:10.1093/jas/sky458

Cited by 16 publications

(8 citation statements)

References 49 publications

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“…The other prominent steroidogenic enzyme present in the endometrium was HSD3B2. Previously known as HSD3B1, this enzyme has been reported in the equine chorioallantois [32] and testis [54], as well as early pregnant porcine endometrium[55], but hasn’t previously been studied in the equine endometrium. In contrast, INHBA has been previously reported to be endometrium-specific [56], although these data suggest that it is also expressed at a lower, yet still significant, level in the chorioallantois (Table 2).…”

Section: Discussionmentioning

confidence: 99%

Characterization of the placental transcriptome through mid to late gestation in the mare

et al. 2019

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The placenta is a dynamic organ which undergoes extensive remodeling throughout pregnancy to support, protect and nourish the developing fetus. Despite the importance of the placenta, very little is known about its gene expression beyond very early pregnancy and post-partum. Therefore, we utilized RNA-sequencing to characterize the transcriptome from the fetal (chorioallantois) and maternal (endometrium) components of the placenta from mares throughout gestation (4, 6, 10, 11 m). Within the endometrium, 47% of genes changed throughout pregnancy, while in the chorioallantois, 29% of genes underwent significant changes in expression. Further bioinformatic analyses of both differentially expressed genes and highly expressed genes help reveal similarities and differences between tissues. Overall, the tissues were more similar than different, with ~ 95% of genes expressed in both tissues, and high similarities between the most highly expressed genes (9/20 conserved), as well as marked similarities between the PANTHER pathways identified. The most highly expressed genes fell under a few broad categories, including endocrine and immune-related transcripts, iron-binding proteins, extracellular matrix proteins, transport proteins and antioxidants. Serine protease inhibitors were particularly abundant, including SERPINA3, 6 and 14, as well as SPINK7 and 9. This paper also demonstrates the ability to effectively separate maternal and fetal components of the placenta, with only a minimal amount of chorioallantoic contamination in the endometrium (~8%). This aspect of equine placentation is a boon for better understanding gestational physiology and allows the horse to be used in areas where a separation of fetal and maternal tissues is essential. Overall, these data represent the first large-scale characterization of placental gene expression in any species and include time points from multiple mid- to late-gestational stages, helping further our understanding of gestational physiology.

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Section: Discussionmentioning

confidence: 99%

Characterization of the placental transcriptome through mid to late gestation in the mare

et al. 2019

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“…However, the residual data obtained in our previous studies seem to confirm that OXB participates in the expression of uterine genes and macromolecules biosynthesis processes. Our previous research revealed that OXB exerted a dose‐dependent influence on the expression of genes encoding enzymes that are crucial for steroidogenesis, as well as the production and secretion of steroids such as testosterone, progesterone and oestradiol in the porcine uterus during early gestation (Kaminski et al., 2018; Kisielewska et al., 2019). An analysis of the interactions between DEG also confirmed that OXB affects the genes linked with lipid transport, steroid metabolism, hormone binding and regulation of hormone levels, which seems to confirm that OXB modulates endometrial gene expression and the biosynthesis of macromolecules, including key steroid enzymes and factors.…”

Section: Discussionmentioning

confidence: 99%

“…The above suggests that orexins' effects could be modulated by specific local factors in different phases of the oestrous cycle or pregnancy (Nitkiewicz et al., 2012; Smolinska et al., 2015). Our previous studies demonstrated that OXB modulates the expression of genes encoding crucial steroidogenic enzymes and proteins, including the cytochrome P450 side‐chain cleavage enzyme ( CYP11A1 ), 3β‐hydroxysteroid dehydrogenase ( HSD3B1 ), steroidogenic acute regulatory protein ( StAR ), cytochrome P450 aromatase ( CYP19A3 ) and cytochrome P450c17 ( CYP17A1 ), and influences the secretion of progesterone, androstenedione, testosterone, oestrone and oestradiol by porcine uterine tissues during early gestation and in the luteal phase of the oestrous cycle (Kaminski et al., 2018; Kisielewska et al., 2019).…”

Section: Introductionmentioning

confidence: 99%

Orexin B affects the transcriptome of incubated in vitro porcine endometrial explants from the early‐implantation period

Dobrzyń

Kieżun

Szeszko

et al. 2020

Reprod Domestic Animals

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This study determined the effect of orexin B (OXB) on the porcine endometrial transcriptome during the embryo attachment phase. Microarray analyses of gene ontology (GO), biological pathways, networks and differentially expressed genes (DEG) were performed. Orexin B influenced the expression of 887 genes (fold change > 1.2; p < .05): 620 genes were up‐regulated, and 267 were down‐regulated. The analysis of the relationship between DEG revealed that OXB interacts with genes linked with processes such as cell hormone binding, regulation of hormone levels, lipid transport, steroid metabolic processes, the apoptotic signalling pathway and the acute inflammatory response, which are pivotal for reproductive success. Orexin B played a bivalent role in the early‐pregnant uterus by limiting the pregnancy outcome, promoting embryo development, suppressing the immune system and, consequently, preventing embryo rejection. These findings suggest that OXB could be responsible for the proper course of gestation by adapting litter size to the metabolic status of the maternal organism.

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“…In contrast, the role of OXB and OX2R in the regulation of reproductive activity has been poorly investigated. Kaminski et al [ 18 ] and Kisielewska et al [ 19 ] documented an involvement of OXB in the regulation of steroidogenesis by the porcine uterus during the luteal phase and early pregnancy. As for the gonads, Liguori et al [ 20 ] provided the first evidence of OXB and OXR2 localization within the rat epididymis.…”

Section: Introductionmentioning

confidence: 99%

Effects of Orexin B on Swine Granulosa and Endothelial Cells

Grasselli

Bussolati

Grolli

et al. 2021

Animals

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In addition to the well-known central modulatory role of orexins, we recently demonstrated a peripheral involvement in swine granulosa cells for orexin A and in adipose tissue for orexin B (OXB). The aim of present research was to verify immunolocalization of OXB and its potential role in modulating the main features of swine granulosa cells. In particular, we explored the effects on granulosa cell proliferation (through the incorporation of bromodeoxyuridine), cell metabolic activity (as indirect evaluation by the assessment of ATP), steroidogenic activity (by immunoenzymatic examination) and redox status (evaluating the production of superoxide anion by means of the WST test, production of nitric oxide through the use of the Griess test and the non-enzymatic reducing power by FRAP test). Our data point out that OXB does not modify granulosa cell growth, steroidogenesis and superoxide anion generation. On the contrary, the peptide stimulates (p < 0.05) nitric oxide output and non-enzymatic reducing power. Since new vessel growth is crucial for ovarian follicle development, a further aim of this study was to explore the expression of prepro-orexin and the effects of OXB on swine aortic endothelial cells. We found that the peptide is ineffective in modulating cell growth, while it inhibits redox status parameters. In addition, we demonstrated a stimulatory effect on angiogenesis evaluated in fibrin gel angiogenesis assay. Taken together, OXB appears to be potentially involved in the modulation of redox status in granulosa and endothelial cells and we could argue an involvement of the peptide in the follicular angiogenic events.

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The effect of orexin B on steroidogenic acute regulatory protein, P450 side-chain cleavage enzyme, and 3β-hydroxysteroid dehydrogenase gene expression, and progesterone and androstenedione secretion by the porcine uterus during early pregnancy and the estrous cycle1

Cited by 16 publications

References 49 publications

Characterization of the placental transcriptome through mid to late gestation in the mare

Characterization of the placental transcriptome through mid to late gestation in the mare

Orexin B affects the transcriptome of incubated in vitro porcine endometrial explants from the early‐implantation period

Effects of Orexin B on Swine Granulosa and Endothelial Cells

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