The effect of operational parameters on the biodegradation of bisphenols by Trametes versicolor laccase immobilized on Hippospongia communis spongin scaffolds

Zdarta, Jakub; Antecka, Katarzyna; Frankowski, Robert; Zgoła‐Grześkowiak, Agnieszka; Ehrlich, Hermann; Jesionowski, Teofil

doi:10.1016/j.scitotenv.2017.09.213

Cited by 156 publications

(68 citation statements)

References 78 publications

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“…Laccases (EC 1.10.3.2) are a promising group of oxidoreductases that have high activity in oxidizing phenolic compounds and arylamine, including phenols, polyphenols, methoxy‐substituted phenols, aromatic and aliphatic amines, by using molecular oxygen as the electron acceptor without the need for cofactors (Strong & Claus, ; Thakur, Patel, Gupte, & Gupte, ). Prior studies have demonstrated the use of laccases for effective degradation of emerging contaminants, such as endocrine disruptors (bisphenol A [Zdarta, Antecka et al, ] and triclosan [Le et al, ]), pharmaceuticals (sulfamethoxazole [Margot, Copin, von Gunten, Barry, & Holliger, ] and naproxen [Tran, Urase, & Kusakabe, ]), and pesticides (bromofenoxim [Torres‐Duarte, Roman, Tinoco, & Vazquez‐Duhalt, ] and carbofuran [Wang, Liu, Yao, Zhang, & Bao, ]). However, using free enzymes faces challenges including short enzyme lifetimes, time‐consuming and expensive purification processes, and nonreusability (Sheldon & van Pelt, ).…”

Section: Introductionmentioning

confidence: 99%

Biocatalytic properties of cell surface display laccase for degradation of emerging contaminant acetaminophen in water reclamation

Chen

Wei

2019

Biotech & Bioengineering

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The surface display laccase (SDL) biocatalyst, where the enzyme laccase is displayed on the surface of biological cells through synthetic biology, provides a new opportunity to develop sustainable technologies for removal of emerging contaminants from wastewater. This study vigorously characterized biocatalytic properties of the SDL in comparison to free laccase in removing emerging contaminant acetaminophen (APAP), with the aim to understand the effect of surface display on enzyme functionality and identify the strategy to overcome the potential limitation. The SDL could effectively remove APAP. Adding redox mediators substantially improved the removal efficiency. The Michaelis–Menten kinetic analysis showed that the redox mediator 2,2‐azinobis‐3‐ethylbenzothiazoline‐6‐sulfonate could overcome the limitation of APAP accessing the active site of laccase in the SDL biocatalyst. The APAP removal rate catalyzed by the SDL in real secondary wastewater effluent was higher than that in acetate buffer; comprehensive enzyme kinetic analysis provided clear evidence that there were redox mediating compounds in the wastewater. Analysis of transformation products revealed that surface display did not change laccase functionality in terms of APAP transformation mechanism. In addition, the SDL retained 88% of the initial activity after six repeated APAP biotransformation reactions. Results from this study provide a scientific basis for developing and implementing SDL as an innovative biocatalytic material for contaminant treatment applications.

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Section: Introductionmentioning

confidence: 99%

Biocatalytic properties of cell surface display laccase for degradation of emerging contaminant acetaminophen in water reclamation

Chen

Wei

2019

Biotech & Bioengineering

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“…[6,10] Various techniques and methods of immobilization such as adsorption, covalent binding, entrapment or encapsulation have been described previously. [11][12][13] Silica-based materials are frequently used as support materials in enzyme immobilization due to their thermal, chemical and mechanical resistance, good sorption properties, and the presence of many hydroxyl groups that facilitate enzyme binding. [14][15][16] Moreover, silica-based materials are easy to obtain and relatively cheap, a feature of particular significance in biomass valorization processes.…”

Section: Introductionmentioning

confidence: 99%

Upgrading of Biomass Monosaccharides by Immobilized Glucose Dehydrogenase and Xylose Dehydrogenase

et al. 2018

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Direct upgrading and separation of the monosaccharides from biomass liquors is an overlooked area. In this work we demonstrate enzymatic production of gluconic acid and xylonic acid from glucose and xylose present in pretreated birchwood liquor by glucose dehydrogenase (GDH, EC 1.1.1.47) and xylose dehydrogenase (XDH, EC 1.1.1.175), respectively. The biocatalytic conversions were compared using two different kinds of silica support materials (silica nanoparticles (nanoSiO2) and porous silica particles with hexagonal pores (SBA 15 silica) for enzyme immobilization. Upon immobilization, both enzymes showed significant improvement in their thermal stability and robustness at alkaline pH and exhibited over 50 % activity even at pH 10 and 60 °C on both immobilization matrices. When compared to free enzymes at 45 °C, GDH immobilized on nanoSiO2 and SBA silica displayed a 4.5 and 7.25 fold increase in half‐life, respectively, whilst XDH immobilized on nanoSiO2 and SBA showed a 4.7 and 9.5 fold improvement in half‐life, respectively. Additionally, after five reaction cycles both nanoSiO2GDH and nanoSiO2XDH retained more than 40 % activity and GDH and XDH immobilized on SBA silica maintained around 50 % of their initial activity resulting in about 1.5–1.6 fold increase in biocatalytic productivity compared to the free enzymes.

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“…More so, Zdarta et al. () reported approximately 100% removal of BPA and bisphenol F (BPF) by Trametes versicolor laccase immobilized on Hippospongia communis spongin scaffolds. However, the removal efficiency of the enzyme on bisphenol S (BPS) was only greater than 40%.…”

Section: Potential Of Les In the Elimination Of Edc In Wastewatermentioning

confidence: 99%

“…However, addition of H 2 O 2 may also increase laccase activity during oxidation of some phenolic compounds (Min, Kim, Kim, Jung, & Hah, 2001). Although both enzymes usually perform optimally in slightly acidic pH region (Jarosz-Wilkolazka, Luterek, & Olszewska, 2008;Min et al, 2001;Zdarta et al, 2018), it is important to determine the pH requirements of the enzymes to ensure best performance during application. Likewise, efforts should be geared toward assessing the potential toxicity of Mn 2+ and malonate in the effluent and the chance of recovering them after the enzymatic treatment.…”

Section: Prop Os Ed Scheme Of Wa S Te Water Tre Atment Pro Ce Ss Fomentioning

confidence: 99%

Ligninolytic enzymes: Versatile biocatalysts for the elimination of endocrine‐disrupting chemicals in wastewater

et al. 2018

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Direct municipal wastewater effluent discharge from treatment plants has been identified as the major source of endocrine‐disrupting chemicals (EDC) in freshwaters. Consequently, efficient elimination of EDC in wastewater is significant to good water quality. However, conventional wastewater treatment approaches have been deficient in the complete removal of these contaminants. Hence, the exploration of new and more efficient methods for elimination of EDC in wastewater is imperative. Enzymatic treatment approach has been suggested as a suitable option. Nonetheless, ligninolytic enzymes seem to be the most promising group of enzymes for EDC elimination, perhaps, owing to their unique catalytic properties and characteristic high redox potentials for oxidation of a wide spectrum of organic compounds. Therefore, this paper discusses the potential of some ligninolytic enzymes (laccase, manganese peroxidase, and versatile peroxidase) in the elimination of EDC in wastewater and proposes a new scheme of wastewater treatment process for EDC removal.

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The effect of operational parameters on the biodegradation of bisphenols by Trametes versicolor laccase immobilized on Hippospongia communis spongin scaffolds

Cited by 156 publications

References 78 publications

Biocatalytic properties of cell surface display laccase for degradation of emerging contaminant acetaminophen in water reclamation

Biocatalytic properties of cell surface display laccase for degradation of emerging contaminant acetaminophen in water reclamation

Upgrading of Biomass Monosaccharides by Immobilized Glucose Dehydrogenase and Xylose Dehydrogenase

Ligninolytic enzymes: Versatile biocatalysts for the elimination of endocrine‐disrupting chemicals in wastewater

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