The Effect of Oncogene Proteins of Human Papillomaviruses on Apoptosis Pathways in Prostate Cancer

Baladehi, Robabeh Faghani; Memar, Mohammad Yousef; Jafari-Sales, Abolfazl; Bazmani, Ahad; Nahand, Javid Sadri; Aghbash, Parisa Shiri; Baghi, Hossein Bannazadeh

doi:10.32604/oncologie.2022.020648

Cited by 6 publications

(4 citation statements)

References 119 publications

(174 reference statements)

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“…Ferroptosis is a new controllable programmed cell death mode characterized by iron-dependent accumulation of intracellular ROS and lipid ROS, leading to mitochondrial membrane thickening, ridge reduction and cell membrane fragmentation. A series of abnormal gene expression and signal transduction are involved in the process of programmed cell death [ 20 – 22 ]. With the in-depth research on ferroptosis and degenerative diseases in recent years, more and more evidence has demonstrated the close connection between ferroptosis and OA progression.…”

Section: Discussionmentioning

confidence: 99%

Baicalin inhibits IL-1β-induced ferroptosis in human osteoarthritis chondrocytes by activating Nrf-2 signaling pathway

Liu,

Zhou,

Chen

et al. 2024

J Orthop Surg Res

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Background Osteoarthritis (OA) is a common degenerative disease involving articular cartilage, in which ferroptosis of chondrocytes plays an important role. Baicalin (BAI) exerts regulatory effects in a wide range of orthopedic diseases including OA, but its effect on ferroptosis of chondrocytes (CHs) is still unclear. The purpose of this study was to determine the effect of BAI on ferroptosis in human OA chondrocytes (OACs), and to explore its possible mechanism. Methods CHs were treated with IL-1β (10 ng/mL) to simulate inflammation in vitro. Immunofluorescence, quantitative RT-PCR, Western blotting and cell viability assay were performed to evaluate the impacts of BAI on Fe2+ level, mitochondrial dysfunction, ferroptosis-related proteins, oxidative stress and cytotoxicity in CHs. Additionally, siRNA was made use of to knock out nuclear factor E2-related factor 2 (Nrf2) to analyze the role played by Nrf2 in BAI-induced CH ferroptosis. Results BAI eliminated IL-1β-induced Fe2+ accumulation, changes in mitochondrial membrane potential and ferroptosis-related protein GPX4, SLC7A11, P53 and ACSL4 levels, as well as reactive oxygen species (ROS), lipid peroxidation (LPO) and malondialdehyde (MDA) accumulation in CHs. Besides, BAI reversed IL-1β-induced decrease of Collagen II and increase of MMP13 in CHs. Meanwhile, BAI attenuated IL-1β-induced CH toxicity and promoted Nrf2 antioxidant system activation. When Nrf2 was knocked down by siRNA, the effects of BAI on IL-1β-induced ferroptosis-related proteins and antioxidant stress in CHs were significantly weakened. Conclusions This study demonstrates that IL-1β can induce CH ferroptosis. BAI is able to inhibit IL-1β-induced CH ferroptosis and ECM degradation, and the specific mechanism may be that it can inhibit IL-1β-induced CH ferroptosis by activating Nrf2 antioxidant system to attenuate the accumulation of intracellular ROS and lipid ROS.

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Section: Discussionmentioning

confidence: 99%

Baicalin inhibits IL-1β-induced ferroptosis in human osteoarthritis chondrocytes by activating Nrf-2 signaling pathway

Liu,

Zhou,

Chen

et al. 2024

J Orthop Surg Res

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“…COX-2 derived prostaglandin at chronic inflammatory diseases may lead to cancer [16]. Multiple signaling pathways are activated by COX-2-induced synthesis of prostaglandins in cancer cells, including apoptosis pathway [17][18][19][20]. High COX-2 expression is detected in a variety of cancers, and is closely related to the characteristics of malignant tumor features and poor prognosis [21].…”

Section: Discussionmentioning

confidence: 99%

Brucine suppresses proliferation and promotes apoptosis of human cholangiacarcinoma cells via the inhibition of COX2 expression

Kang,

Zheng,

Jiang

et al. 2023

J. Cancer

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Aims The aim of this study was to investigate the anti-tumor efficacy of brucine on intrahepatic cholangiocarcinoma (ICC). Methods ICC QBC939 cells were treated with brucine, cell viability, cell cycle and apoptosis were analyzed using CCK-8 and flow cytometry. The expression of COX-2 and apoptosis related proteins Casp3, Bax and Bcl-2 were detected by Western blot analysis. QBC939 cells were subcutaneously transplanted into nude mice and the mice were injected with brucine intraperitoneally. The expression of Ki67, COX-2 and apoptosis related proteins were detected by immunohistochemical staining and Western blot analysis. Results Brucine significantly inhibited the proliferation and cell cycle progression while promoted the apoptosis of QBC939 cells. The expression of the apoptotic proteins Casp3 and Bax was upregulated, while the expression of Bcl-2 and COX-2 was downregulated in QBC939 cells with brucine treatment. Moreover, the overexpression of COX-2 could antagonize the effects of brucine on QBC939 cells. In vivo , brucine inhibited subcutaneous tumor formation in nude mice, and the expression of Ki67, COX-2 and Bcl-2 decreased while the expression of Casp3 and Bax increased in tumor tissues from nude mice with brucine treatment. Conclusions Brucine can significantly inhibit the progression of cholangiocarcinoma in vitro and in vivo , and the mechanism may be related to the inhibition of COX-2 expression.

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“…Scanpy v. 1.8.1 ( https://pypi.org/project/scanpy/ ) under Python v. 3.7 ( https://www.python.org/downloads/ ) was used for quality control, dimensionality reduction, and clustering. The expression matrix was filtered for each sample dataset, according to the following exclusion criteria: 1) cells with gene count <200 and the top 2% gene counts; 2) cells with the top 2% UMI counts; 3) cells with mitochondrial content >10%; and 4) genes expressed in fewer than 5 cells ( Satija et al, 2015 ; Baladehi et al, 2022 ). After filtering, 49,027 cells were retained for the downstream analyses, and there were averages of 2,146 genes and 8,911 UMIs/cell.…”

Section: Methodsmentioning

confidence: 99%

Cascading effects of hypobaric hypoxia on the testis: insights from a single-cell RNA sequencing analysis

Ji,

Wang,

Zhang

et al. 2023

Front. Cell Dev. Biol.

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Most mammals tolerate exposure to hypobaric hypoxia poorly as it may affect multiple regulatory mechanisms and inhibit cell proliferation, promote apoptosis, limit tissue vascularization, and disrupt the acid–base equilibrium. Here, we quantified the functional state of germ cell development and demonstrated the interaction between the germ and somatic cells via single-cell RNA sequencing (scRNA-seq). The present study elucidated the regulatory effects of hypobaric hypoxia exposure on germ cell formation and sperm differentiation by applying enrichment analysis to genomic regions. Hypobaric hypoxia downregulates the genes controlling granule secretion and organic matter biosynthesis, upregulates tektin 1 (TEKT1) and kinesin family member 2C (KIF2C), and downregulates 60S ribosomal protein 11 (RPL11) and cilia- and flagella-associated protein 206 (CFAP206). Our research indicated that prosaposin-G protein-coupled receptor 37 (PSAP-GPR37) ligands mediate the damage to supporting cells caused by hypobaric hypoxic exposure. The present work revealed that hypoxia injures peritubular myoid (PTM) cells and spermatocytes in the S phase. It also showed that elongating spermatids promote maturation toward the G2 phase and increase their functional reserve for sperm–egg binding. The results of this study provide a theoretical basis for future investigations on prophylactic and therapeutic approaches toward protecting the reproductive system against the harmful effects of hypobaric hypoxic exposure.

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The Effect of Oncogene Proteins of Human Papillomaviruses on Apoptosis Pathways in Prostate Cancer

Cited by 6 publications

References 119 publications

Baicalin inhibits IL-1β-induced ferroptosis in human osteoarthritis chondrocytes by activating Nrf-2 signaling pathway

Baicalin inhibits IL-1β-induced ferroptosis in human osteoarthritis chondrocytes by activating Nrf-2 signaling pathway

Brucine suppresses proliferation and promotes apoptosis of human cholangiacarcinoma cells via the inhibition of COX2 expression

Cascading effects of hypobaric hypoxia on the testis: insights from a single-cell RNA sequencing analysis

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