The effect of obstructive jaundice on the migration of reticulo-endothelial cells and fibroblasts into early experimental granulomata

Abstract

Clinical and experimental evidence indicates that obstructive jaundice inhibits the healing of wounds. Experiments in the rat designed to investigate the migration of reticulo‐endothelial and fibroblasts into experimental granulomata are described. Standard preparations of obstructive jaundice and controls were devised using Wistar rats. The effects of bile‐duct obstruction and sham ligation are described. In another group of animals which was similarly prepared granuloma‐pouch experiments were carried out by burying sterile cotton‐wool pledgets of a known weight. These peledgets were removed from the jaundiced and control animals on the third, fifth, seventh, and ninth days after implantation, dried to remove the fluid component of the inflammatory process, and weighed on a microbalance. The weights were compared and it was found that a significant inhibition of weight increase occurred in the jaundiced animals during the first week, suggesting a delay in the migratory activity of reticuloendothelial cells and fibroblasts.

Hepatocellular apoptosis after hepatectomy in obstructive jaundice in rats

“…After ligation of the proximal and distal bile ducts, the common bile duct was divided to prevent recanalization [10] . The ligatures were placed in the same position in all rats.…”

Viability and Plasma Vitamin K Levels in the Common Bile Duct-Ligated Rats

“…Therefore the viability of this model is poor. Lee et al improved the viability of CBDL rats by supplying a diet enriched with protein, ascorbic acid and vitamin K [20]. Beck reduced the mortality rate of this model from 20-25% to 10% within 4 weeks of operation by subcutaneous…”

Evaluation of the effects of Aegle marmelos and Punica granatum in an experimental model of gastrointestinal barrier dysfunction

“…In group 1 (sham operated), the common bile duct was identified, freed from the surrounding soft tissue and manipulated without carrying out ligation and transection. In groups 2-7, the common bile duct was dissected and double-ligated with 6/0 silk and sectioned between the ligatures according to the procedure described by Lee et al 11 All abdominal incisions were closed in 2 layers using 4/0 chromic catgut. After surgery, the rats were allowed to recover from anaesthesia following which each rat was housed in an individual cage with free access to food and water.…”