Nutritionally important chemical constituents and yield of carrot (Daucus carota L.) roots grown organically using ten levels of green manure. Accepted February 27, 2002. Acta Agric. Scand., Sect. B, Soil and Plant Sci. 51: 125 -136, 2001. © 2002 Taylor & Francis.In a eld experiment carried out over two years (1995, 1996) carrots were grown organically on a sandy loam soil. A broad range of mineral nitrogen (N-min) levels at carrot emergence was obtained by depleting or amending the soil by removing or supplying different amounts of green manure. With N-min values ranging from 22 to 162 kg N ha ¼ 1 at carrot emergence, the N uptake of the carrot crop increased linearly from 70 to 200 kg N ha ¼ 1 . The yield increased with N-min at lower levels of N, but levelled off above 90 -100 kg N ha ¼ 1 . In fresh carrot roots the concentrations of total N, ten individual amino acids, total sum of amino acids, two amides, asparagine and glutamine, and b-carotene increased linearly with the soil concentration of N-min at carrot emergence. Nitrate N increased exponentially with N-min, reaching levels of 340 mg NO 3 kg ¼ 1 fresh weight under some conditions. The concentrations of potassium, calcium, glucose, fructose, sucrose and six individual free amino acids were unaffected by the N-min level. Magnesium was decreased at the lowest deliberately depleted N-min levels, whereas the concentration of dry matter and vitamin C decreased linearly with increasing N-min levels. Signi cantly lower concentrations of dry matter, total N, nitrate N, vitamin C and total sum of free amino acids were found in the warm and sunny year 1995 with the highest yield of carrot roots, whereas the contrary was found for b-carotene.