The effect of neurotrophin-3/chitosan carriers on the proliferation and differentiation of neural stem cells

Li, Xiaoguang; Yang, Zhaoyang; Zhang, Aifeng

doi:10.1016/j.biomaterials.2009.05.047

Cited by 81 publications

(54 citation statements)

References 23 publications

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“…The chitosan tubes and NT3-chitosan carriers were prepared as described previously (17,18,24), with modifications. Detailed information is provided in SI Materials and Methods.…”

Section: Methodsmentioning

confidence: 99%

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NT3-chitosan elicits robust endogenous neurogenesis to enable functional recovery after spinal cord injury

Yang

Zhang

Duan

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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Neural stem cells (NSCs) in the adult mammalian central nervous system (CNS) hold the key to neural regeneration through proper activation, differentiation, and maturation, to establish nascent neural networks, which can be integrated into damaged neural circuits to repair function. However, the CNS injury microenvironment is often inhibitory and inflammatory, limiting the ability of activated NSCs to differentiate into neurons and form nascent circuits. Here we report that neurotrophin-3 (NT3)-coupled chitosan biomaterial, when inserted into a 5-mm gap of completely transected and excised rat thoracic spinal cord, elicited robust activation of endogenous NSCs in the injured spinal cord. Through slow release of NT3, the biomaterial attracted NSCs to migrate into the lesion area, differentiate into neurons, and form functional neural networks, which interconnected severed ascending and descending axons, resulting in sensory and motor behavioral recovery. Our study suggests that enhancing endogenous neurogenesis could be a novel strategy for treatment of spinal cord injury.spinal cord injury | NT3 | chitosan | functional recovery | endogenous neurogenesis

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“…The chitosan tubes and NT3-chitosan carriers were prepared as described previously (17,18,24), with modifications. Detailed information is provided in SI Materials and Methods.…”

Section: Methodsmentioning

confidence: 99%

“…We have previously shown that neurotrophin-3 (NT3) facilitates proliferation of NSCs in vitro through activation of the TrkC receptor and promotes neurogenesis in the spinal cord (17,18). NT3 has also been shown to facilitate axonal growth, including in the CST, in vivo (19).…”

mentioning

confidence: 99%

NT3-chitosan elicits robust endogenous neurogenesis to enable functional recovery after spinal cord injury

Yang

Zhang

Duan

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

173

172

View full text Add to dashboard Cite

show abstract

“…The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Sigma) method (Li et al 2009) was used to detect cell metabolic activity. One hundred microliter of the single-cell suspension from 5, 7 and 9-days primary cultured neurosheres were inoculated into individual wells of 96-well culture plates (Corning) at a density of 5 9 10 5 cells/mL.…”

Section: Isolation and Cultivation Of Nscsmentioning

confidence: 99%

Optimal time for passaging neurospheres based on primary neural stem cell cultures

et al. 2011

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Cultured neural stem cells (NSCs) provide a powerful means for investigating central nervous system disease, neuron development, differentiation, and regeneration. To obtain sufficient neurospheres, subculturing is essential following establishment of the primary NSC culture. Passaging the primary neurospheres is a key issue that is often ignored. We evaluated the influence of different passaging schedules on primary cultured NSCs. Passaging was performed on day 5, 7 or 9. We observed more neurospheres with diameters of 200-250 lm on day 7 than on day 5 or 9. Prolonging the time of primary culture reduced the cell metabolic activity by the MTT assay and cell proliferation by colony-forming assay and the differentiation to neurons from cells at P2 and later decreased. Additionally, more cells were in G0/G1 phase, and higher expression of p16INK4a and lower expression of cyclin D1 was found when the time of primary culture was prolonged to 9 days compared to 7-days cultures. Thus, in this study, we established that the optimal time for subculturing aggregated NSCs was on day 7 based on the primary culture.

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“…Therefore, they can be incorporated within hydrogels to induce neural differentiation. For instance, through incorporation of neurotrophin-3 (NT-3) into chitosan hydrogels, NSC differentiation toward neurons was achieved (Li et al 2009 ). On the other hand, when FGF-2 was incorporated into PEG hydrogels, NSCs preferred to stay at undifferentiated state in spite of the addition of differentiation medium (Freudenberg et al 2009 ).…”

Section: Chemical Signalsmentioning

confidence: 99%

Bioactive Nanomaterials for Neural Engineering

et al. 2016

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The effect of neurotrophin-3/chitosan carriers on the proliferation and differentiation of neural stem cells

Cited by 81 publications

References 23 publications

NT3-chitosan elicits robust endogenous neurogenesis to enable functional recovery after spinal cord injury

NT3-chitosan elicits robust endogenous neurogenesis to enable functional recovery after spinal cord injury

Optimal time for passaging neurospheres based on primary neural stem cell cultures

Bioactive Nanomaterials for Neural Engineering

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