The effect of neuronal expression of heat shock proteins 26 and 27 on lifespan, neurodegeneration, and apoptosis in Drosophila

Liao, Pin-Chao; Lin, Hung‐Yu; Yuh, Chiou-Hwa; Yu, Lin-Kwei; Wang, Horng-Dar

doi:10.1016/j.bbrc.2008.08.161

Cited by 52 publications

(70 citation statements)

References 43 publications

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“…Increased copy number of hsp70 has been reported to reduce mortality and improve overall survival of organism (Tatar et al 1997). Overexpression of hsp27 (Liao et al 2008) and hsp22 (Kim et al 2010) were shown to provide increased longevity to flies. We observed a significant increase in hsp22, hsp27, and hsp70 expression in the flies that were exposed to DCA up to 48 h. However, in flies that were exposed to DCA for a prolonged period, hsp27 expression was significantly upregulated in 30-50-dayold organism.…”

Section: Discussionmentioning

confidence: 99%

“…Genome-wide studies on age-associated gene expression changes in flies have shown the upregulation of heat shock genes (hsps) (Curtis et al 2007;Landis et al 2012). In addition, modulated expression of hsps (hsp70, hsp27, and hsp22) has been reported to alter life span in flies (Kim et al 2010;Liao et al 2008;Tatar et al 1997) and higher level of HSPs is reported in longerlived mammals and birds (Salway et al 2011). In the same context, improved health-and life span were observed in DCA-exposed Caenorhabditis elegans (Schaffer et al 2011).…”

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confidence: 99%

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Long-term dietary exposure to low concentration of dichloroacetic acid promoted longevity and attenuated cellular and functional declines in aged Drosophila melanogaster

Pandey

Vimal

Chandra

et al. 2014

AGE

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Dichloroacetic acid (DCA), a water disinfection by-product, has attained emphasis due to its prospect for clinical use against different diseases including cancer along with negative impact on organisms. However, these reports are based on the toxicological as well clinical data using comparatively higher concentrations of DCA without much of environmental relevance. Here, we evaluate cellular as well as organismal effects of DCA at environmentally and mild clinically relevant concentrations (0.02-20.0 μg/ml) using an established model organism, Drosophila melanogaster. Flies were fed on food mixed with test concentrations of DCA for 12-48 h to examine the induction of reactive oxygen species (ROS) generation, oxidative stress (OS), heat shock genes (hsps) and cell death along with organismal responses. We also examined locomotor performance, ROS generation, glutathione (GSH) depletion, expression of GSH-synthesizing genes (gclc and gclm), and hsps at different days (0, 10, 20, 30, 40, 50) of the age in flies after prolonged DCA exposure. We observed mild OS and induction of antioxidant defense system in 20.0 μg/ml DCA-exposed organism after 24 h. After prolonged exposure to DCA, exposed organism exhibited improved survival, elevated expression of hsp27, gclc, and gclm concomitant with lower ROS generation and GSH depletion and improved locomotor performance. Conversely, hsp27 knockdown flies exhibited reversal of the above end points. The study provides evidence for the attenuation of cellular and functional decline in aged Drosophila after prolonged DCA exposure and the effect of hsp27 modulation which further incites studies towards the therapeutic application of DCA.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Long-term dietary exposure to low concentration of dichloroacetic acid promoted longevity and attenuated cellular and functional declines in aged Drosophila melanogaster

Pandey

Vimal

Chandra

et al. 2014

AGE

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“…The matter of timing has also been reported in other studies. For example, the degree of the protective effect of Hsp26 over-expression is higher in adults than in larvae where it has only a small thermoprotective effect on larval locomotion and no effect on neural function (Liao et al 2008 ;Mileva-Seitz et al 2008 ;Wang et al 2004 ).…”

Section: Shsp Expression In Adult Fliesmentioning

confidence: 99%

“…While reaper and grim are expressed only in cells destined to die, hid is also present during development and its expression is regulated by EGFR/RAS signaling, Hippo tumor suppressor pathway, miRNAs and the hormone ecdysone (Bilak and Su 2009 ). Hsp27 reduces hid-induced lethality but has no effect on the lethality induced by reaper and grim (Liao et al 2008 ). The exact mechanism by which Hsp27 protects Drosophila against hid-induced apoptosis is not known but it could be by regulating the Ras/MAPK pathway in a similar way to Hsp83 i.e.…”

Section: Shsps and Apoptosismentioning

confidence: 99%

“…While Hsp26 has no effect on fl y survival to hid-, reaper-and grim-induced lethality when over-expressed in neurons (Liao et al 2008 ), it was shown to interact with activated Cdc42 kinase (ack) together with Hsp23 and some other proteins in an affi nity assay on S2 cells (Schoenherr et al 2012 ). Ack is an anti-apoptotic protein, which blocks programmed cell death induced by hid and reaper.…”

Section: Shsps and Apoptosismentioning

confidence: 99%

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Drosophila Small Heat Shock Proteins: An Update on Their Features and Functions

Morrow¹,

Tanguay²

2015

Heat Shock Proteins

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Small heat shock proteins (sHsps) are present in varying numbers in all organisms. In Drosophila melanogaster there are 12 sHsps, which have distinctive developmental expression patterns, intracellular localizations and substrate specifi cities. Even if most of Drosophila sHsps do not have a known mammalian ortholog, they share their involvement in multiple cellular processes such as cytoskeleton modulation, apoptosis and autophagy. New data on Drosophila sHsps have arisen from high-throughput genomic and proteomic studies as well as from deletion experiments. In addition to showing the complexity of this family, these experiments suggest the involvement of sHsps in new cellular processes such as the involvement of Hsp27 in piRNA biosynthesis. The goal of this review is to summarize the new fi ndings on each Drosophila sHsp and to highlight its similarity to other sHsps as well as its distinctive features.

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The Big Book on Small Heat Shock Proteins

Hightower¹,

Tanguay²

2015

Heat Shock Proteins

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Heat Shock Proteins: key mediators of Health and Disease. Heat shock proteins (HSP) are essential molecules conserved through cellular evolution required for cells to survive the stresses encountered in the environment and in the tissues of the developing and aging organism. These proteins play the essential roles in stress of preventing the initiation of programmed cell death and repairing damage to the proteome permitting resumption of normal metabolism. Loss of the HSP is lethal either in the short-term in cases of acute stress or in the long-term when exposure to stress is chronic. Cells appear to walk a fi ne line in terms of HSP expression. If expression falls below a certain level, cells become sensitive to oxidative damage that infl uences aging and protein aggregation disease. If HSP levels rise above the normal range, infl ammatory and oncogenic changes occur. It is becoming clear that HSP are emerging as remarkably versatile mediators of health and disease. The aim of this series of volumes is to examine how HSP regulation and expression become altered in pathological states and how this may be remedied by pharmacological and other interventions.More information about this series at

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The effect of neuronal expression of heat shock proteins 26 and 27 on lifespan, neurodegeneration, and apoptosis in Drosophila

Cited by 52 publications

References 43 publications

Long-term dietary exposure to low concentration of dichloroacetic acid promoted longevity and attenuated cellular and functional declines in aged Drosophila melanogaster

Long-term dietary exposure to low concentration of dichloroacetic acid promoted longevity and attenuated cellular and functional declines in aged Drosophila melanogaster

Drosophila Small Heat Shock Proteins: An Update on Their Features and Functions

The Big Book on Small Heat Shock Proteins

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