The Effect of Natrium Hyaluronate Applied Locally for Pelviureteral Anastomoses

Abstract

Objectives: This study aimed to investigate the effect of natrium hyaluronate (NH) on fibrous tissue formation and wound healing in experimental pelviureteral anastomosis (PUA). Materials and methods: Eighteen rabbits were divided equally into three groups: surgical (S), sham (Sh), and NH. A 1-cm length of the ureteropelvic segment was resected through a laparotomy incision and then anastomosis was performed. The rabbits were injected with saline (Sh group) and NH (NH group) into anastomoses lines after the surgical procedure. The S group did not receive any medication during their procedure. Intravenous pyelography was carried out on postoperative day 21. The rabbits were sacrificed and dissected under a dissecting microscope and examined for acute inflammation (AI), chronic inflammation (CI), granulation tissue amount (GTA), granulation tissue fibroblast maturation (GTFM), collagen deposition (CD), neovascularization (N), re-epithelialization (R), and peripheral tissue reaction (PTR) in the anastomosis lines 3 weeks later. Main findings: There were no significant differences in the GTFM scores in the S group compared with those in the NH group. In the NH group, N scores were higher than they were in the S group. Re-epithelialization in the NH group was higher than it was in the S group. Principle conclusions: NH did not decrease fibrosis, but increased important parameters in wound healing such as neovascularization and re-epithelialization in an experimental model of PUA in rabbits.

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Immunohistochemical Studies of Age-Related Changes in Cell Proliferation and Angiogenesis during the Healing of Acetic Acid-Induced Gastric Ulcers in Rats

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Effect of a new cross-linked hyaluronan gel on the staple line after sleeve gastrectomy in a rat model

“…Hellstrom et al 10 showed that hyaluronan-treated perforations closed more rapidly than did untreated perforations in a model of induced tympanic membrane perforations in rats. Yurtçu et al 11 reported that hyaluronan facilitated wound healing by increasing re-epithelialization and neovascularization in an experimental model of pelviureteral anastomosis in rabbits. Lan et al 13 reported that hyaluronan suppressed colonization, growth, and metastasis of a gastric cancer cell line in an experimental model.…”

“…application, including in patients with skin damage, chronic venous leg ulcers, tympanic membrane perforation, and pelviureteral anastomoses [8][9][10][11] . Hyaluronan is used to prevent postoperative adhesions because it creates a physiological barrier between the healing tissue and other tissue surfaces during peritoneal reepithelialization 12 .…”