The effect of nanoparticle surface charge on freshwater algae growth, reproduction, and lipid production

Abstract

Using functionalized carbon dots as models, positively charged nanoparticles were found to cause growth inhibition and aggregation in microalgae. Negative and near-neutral negative particles were associated with morphological changes.

Features of the microalga Raphidocelis subcapitata: physiology and applications

“…For this purpose, different cellular and sub-cellular targets/endpoints have been considered (Fig. 4), such as (1) size/biovolume and shape Soares 2014, 2020;Ostovich et al 2023;McKeel et al 2024); (2) membrane integrity (viability) (Nagai et al 2011;Soares 2012a, 2015b); (3) metabolic (esterase) activity (Debenest et al 2012;Machado and Soares 2013;Peng et al 2019;Ciccia et al 2023); (4) macromolecules content and distribution: proteins, carbohydrates, and lipids (Moreira et al 2020;Ciccia et al 2023;Ostovich et al 2023); (5) photosynthetic performance and pigment content (chla, chlb, and carotenoids) (Debenest et al 2012;Peng et al 2019;Almeida et al 2021;dos Reis et al 2022); (6) cell cycle and DNA content (Machado and Soares 2014;Sousa et al 2018;Almeida et al 2019Almeida et al , 2021); (7) mitochondrial membrane potential (Machado and Soares 2015a;Almeida et al 2019;Ciccia et al 2023); (8) oxidative stress assessment: ROS production, enzymatic (superoxide dismutase and catalase activity) and non-enzymatic (reduced glutathione) defenses and lipid peroxidation Soares 2012b, 2021b;Almeida et al 2019;Filová et al 2021); and (9) molecular (transcriptomic and metabolomics) analysis (Guo et al 2021;Mizukami-Murata et al 2021;Gómez-Martínez et al 2023).…”