The Effect of Nanoparticle Size on
            <i>In Vivo</i>
            Pharmacokinetics and Cellular Interaction

Hoshyar, Nazanin; Gray, Samantha; Han, Hongbin; Bao, Gang

doi:10.2217/nnm.16.5

Cited by 1,396 publications

(1,006 citation statements)

References 96 publications

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“…Studies have shown that the cytotoxicity of the drug was inversely proportional to the size of the liposomes . Size of the nanoparticles is crucial for internalization into cells and a size range between 10–60 nm in diameter is reported be ideal for endocytosis . Additionally, a protein corona on the surface of the nanoparticle enhances the endocytosis of the particles into cells with the help of various receptor ligand interactions .…”

Section: Resultsmentioning

confidence: 99%

Molecular Entrapment in Thermophilic Ferritin for Nanoformulation in Photodynamic Therapy

Pasula

Kuniyil

Lim

2020

Advanced Therapeutics

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Nanoformulation of therapeutic and diagnostic agents is beneficial as they accumulate at tumor sites due to enhanced permeability and retention effects. However, many in vitro studies performed on 2D cultures lack the realistic dimension of in vivo systems. In this work, a photosensitizer, acridine orange (AO), used in photodynamic therapy of cancer, is nanoformulated by entrapment in thermophilic ferritin. The efficacy is tested on 2D and 3D in vitro models and complemented with studies on the cellular uptake routes. Ferritin from the archaeon Archaeoglobus fulgidus (AfFtn) exhibits the unique property of divalent metal ion or ionic strength mediated assembly, making it an interesting nanocarrier for the entrapment of small molecules. The photosensitization and toxicity studies on 2D monolayers and 3D spheroid models of colorectal cancer cells show that AO loaded in AfFtn is functional. The killing efficiency in 2D monolayers reaches 50% while the growth of 3D spheroids is arrested after the first day with a subsequent 10% reduction of tumor diameter over the next 2 days. Results show that AO loaded AfFtn has better penetration ability than free AO alone in 3D spheroid models indicating that nanocage formulation provides for an efficient delivery vehicle into the tumor tissue.

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Section: Resultsmentioning

confidence: 99%

Molecular Entrapment in Thermophilic Ferritin for Nanoformulation in Photodynamic Therapy

Pasula

Kuniyil

Lim

2020

Advanced Therapeutics

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“…These results indicated that the developed nanoparticulate system could be suitable for biological purposes. In general, slightly negatively charged nanoparticles have demonstrated excellent cellular uptake, reduced phagocytosis, minimization of nonspecific interactions, etc …”

Section: Resultsmentioning

confidence: 99%

Colloidally stable polypeptide‐based nanogel: Study of enzyme‐mediated nanogelation in inverse miniemulsion

Dvořáková

Šálek

Korecká

et al. 2019

J of Applied Polymer Sci

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The current work presents a pivotal study of the nanogelation of the linear poly(N5‐2‐hydroxypropyl‐L‐glutamine) polymer precursor containing tyramine (TYR) units in an inverse miniemulsion by horseradish peroxidase/H2O2‐mediated crosslinking. The effects of various nH2O2/nTYR ratios on the kinetics of nanogelation in the inverse miniemulsion and on the reaction time are investigated by linear sweep voltammetry, while the formation of dityramine crosslinking is explored by fluorescence spectroscopy. The study is completed using dynamic light scattering measurements, nanoparticle tracking analysis, and cryogenic transmission electron microscopy to acquire comprehensive information about the formed nanoparticulate systems. With the optimal ratio nH2O2/nTYR = 2, the strategy yields in the high‐quality ~ 130 nm poly(amino acid)‐based nanogel, which is prepared in 2 h. The nanogel is colloidally stable under different temperature and pH conditions for over 168 h. Moreover, the demonstrated nanogel is noncytotoxic for HeLa cells and human primary fibroblasts and is quickly enzymatically hydrolyzed into small fragments during a biodegradation study in human blood plasma. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020, 137, 48725.

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“…It was previously reported that nanoparticle size affected the biodistribution of nanoparticles and nanoparticles with diameters larger than 200 nm were prone to activate the complement system, be rapidly removed from the blood stream, and accumulate in the liver and spleen 32 . However, PEGylation of nanoparticles, even >500 nm, allow their escape from the macrophage system and prolong their circulation 33 .…”

Section: Resultsmentioning

confidence: 99%

Dual Tumor-Targeting Nanocarrier System for siRNA Delivery Based on pRNA and Modified Chitosan

Hu²,

Zhang

et al. 2017

Molecular Therapy - Nucleic Acids

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Highly specific and efficient delivery of siRNA is still unsatisfactory. Herein, a dual tumor-targeting siRNA delivery system combining pRNA dimers with chitosan nanoparticles (CNPPs) was designed to improve the specificity and efficiency of siRNA delivery. In this dual delivery system, folate-conjugated and PEGylated chitosan nanoparticles encapsulating pRNA dimers were used as the first class of delivery system and would selectively deliver intact pRNA dimers near or into target cells. pRNA dimers simultaneously carrying siRNA and targeting aptamer, the second class of delivery system, would specifically deliver siRNA into the target cells via aptamer-mediated endocytosis or proper particle size. To certify the delivering efficiency of this dual system, CNPPs, pRNA dimers alone, chitosan nanoparticles containing siRNA with folate conjugation and PEGylation (CNPS), and chitosan nanoparticles containing pRNA dimers alone (CN) were first prepared. Then, we observed that treatment with CNPPs resulted in increased cellular uptake, higher cell apoptosis, stronger cell cytotoxicity, and more efficacious gene silencing compared to the other three formulations. Higher accumulation of siRNA in the tumor site, stronger tumor inhibition, and longer circulating time were also observed with CNPPs compared to other formulations. In conclusion, this dual nanocarrier system showed high targeting and favorable therapeutic efficacy both in vitro and in vivo. Thereby, a new approach is provided in this study for specific and efficient delivery of siRNA, which lays a foundation for the development of pRNA hexamers, which can simultaneously carry six different substances.

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The Effect of Nanoparticle Size on In Vivo Pharmacokinetics and Cellular Interaction

Cited by 1,396 publications

References 96 publications

Molecular Entrapment in Thermophilic Ferritin for Nanoformulation in Photodynamic Therapy

Molecular Entrapment in Thermophilic Ferritin for Nanoformulation in Photodynamic Therapy

Colloidally stable polypeptide‐based nanogel: Study of enzyme‐mediated nanogelation in inverse miniemulsion

Dual Tumor-Targeting Nanocarrier System for siRNA Delivery Based on pRNA and Modified Chitosan

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