The effect of nanofiber-guided cell alignment on the preferential differentiation of neural stem cells

Lim, Shawn; Liu, Xingyu Y.; Song, Hongjun; Yarema, Kevin J.; Mao, Hai‐Quan

doi:10.1016/j.biomaterials.2010.08.021

Cited by 241 publications

(217 citation statements)

References 45 publications

Supporting

Mentioning

207

Contrasting

Order By: Relevance

“…The focus was initially directed to neuronal cells [7,9,11] but more recent studies are contributing to shed some light on the effect of this parameter on other CNS cellular key players, such as astrocytes [14 -17]. It is known that microglia, the immune cells of the CNS, play a critical role in CNS homeostasis as well as being in the frontline of the tissue response to injury [1].…”

Section: Discussionmentioning

confidence: 99%

“…Under the scope of the development of scaffolds for CNS tissue engineering, neurons have been under the spotlight, so far. It has been shown that fibrous surfaces support axonal guidance and growth [7][8][9], as well as stemcell differentiation into the neuronal lineage [10,11]. The number of studies investigating the influence of surface features on glial cells is increasing, yet focused on astrocytes, mainly due to astrocytes' key role in the formation of the glial scar in response to an injury to the CNS [12].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The role of the surface on microglia function: implications for central nervous system tissue engineering

Pires

Rocha

Ambrosio

et al. 2015

J. R. Soc. Interface.

View full text Add to dashboard Cite

In tissue engineering, it is well accepted that a scaffold surface has a decisive impact on cell behaviour. Here we focused on microglia-the resident immune cells of the central nervous system (CNS)-and on their response to poly(trimethylene carbonate-co-1-caprolactone) (P(TMC-CL)) fibrous and flat surfaces obtained by electrospinning and solvent cast, respectively. This study aims to provide cues for the design of instructive surfaces that can contribute to the challenging process of CNS regeneration. Cell morphology was evidently affected by the substrate, mirroring the surface main features. Cells cultured on flat substrates presented a round shape, while cells with elongated processes were observed on the electrospun fibres. A higher concentration of the pro-inflammatory cytokine tumour necrosis factor-a was detected in culture media from microglia on fibres. Still, astrogliosis is not exacerbated when astrocytes are cultured in the presence of microgliaconditioned media obtained from cultures in contact with either substrate. Furthermore, a significant percentage of microglia was found to participate in the process of myelin phagocytosis, with the formation of multinucleated giant cells being observed only on films. Altogether, the results presented suggest that microglia in contact with the tested substrates may contribute to the regeneration process, putting forward P(TMC-CL) substrates as supporting matrices for nerve regeneration.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The role of the surface on microglia function: implications for central nervous system tissue engineering

Pires

Rocha

Ambrosio

et al. 2015

J. R. Soc. Interface.

View full text Add to dashboard Cite

show abstract

“…In addition, the ECM was found to deposit as an extensive scaffold on the basal surface of the cells attached to NFs (Shih et al, 2006;Chua et al, 2007;Ma et al, 2008). Importantly, the sNF system has been reported to enhance not only the differentiation of murine ES cells into neural lineages (Lim et al, 2010;Purcell et al, 2012;Xie et al, 2009), but also differentiation from human MSCs into hepatoblasts (Ghaedi et al, 2012;Kazemnejad et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

A synthetic nanofibrillar matrix promotes in vitro hepatic differentiation of embryonic stem cells and induced pluripotent stem cells

Yamazoe

Shiraki

Toyoda

et al. 2013

Journal of Cell Science

View full text Add to dashboard Cite

SummaryEmbryonic stem (ES) cells recapitulate normal developmental processes and serve as an attractive source for routine access to a large number of cells for research and therapies. We previously reported that ES cells cultured on M15 cells, or a synthesized basement membrane (sBM) substratum, efficiently differentiated into an endodermal fate and subsequently adopted fates of various digestive organs, such as the pancreas and liver. Here, we established a novel hepatic differentiation procedure using the synthetic nanofiber (sNF) as a cell culture scaffold. We first compared endoderm induction and hepatic differentiation between murine ES cells grown on sNF and several other substrata. The functional assays for hepatocytes reveal that the ES cells grown on sNF were directed into hepatic differentiation. To clarify the mechanisms for the promotion of ES cell differentiation in the sNF system, we focused on the function of Rac1, which is a Rho family member protein known to regulate the actin cytoskeleton. We observed the activation of Rac1 in undifferentiated and differentiated ES cells cultured on sNF plates, but not in those cultured on normal plastic plates. We also show that inhibition of Rac1 blocked the potentiating effects of sNF on endoderm and hepatic differentiation throughout the whole differentiation stages. Taken together, our results suggest that morphological changes result in cellular differentiation controlled by Rac1 activation, and that motility is not only the consequence, but is also able to trigger differentiation. In conclusion, we believe that sNF is a promising material that might contribute to tissue engineering and drug delivery.

show abstract

“…Cell alignment along orientated electrospun fibers has been shown previously for different cell types, 79,80 and Lim et.al observed similar behavior as we do for adult neural stem cells. 81 negative cell migration, seen by DAPI staining, out from the neurosphere for spheres on electrospun fiber substrates. The underlying mechanism is currently under investigation.…”

Section: Cell Culture On Electrospun Fibers In a Microfluidic Channelmentioning

confidence: 99%

A method to integrate patterned electrospun fibers with microfluidic systems to generate complex microenvironments for cell culture applications

Wallin

Zandén²,

Carlberg³

et al. 2012

Biomicrofluidics

View full text Add to dashboard Cite

The properties of a cell's microenvironment are one of the main driving forces in cellular fate processes and phenotype expression in vivo. The ability to create controlled cell microenvironments in vitro becomes increasingly important for studying or controlling phenotype expression in tissue engineering and drug discovery applications. This includes the capability to modify material surface properties within well-defined liquid environments in cell culture systems. One successful approach to mimic extra cellular matrix is with porous electrospun polymer fiber scaffolds, while microfluidic networks have been shown to efficiently generate spatially and temporally defined liquid microenvironments. Here, a method to integrate electrospun fibers with microfluidic networks was developed in order to form complex cell microenvironments with the capability to vary relevant parameters. Spatially defined regions of electrospun fibers of both aligned and random orientation were patterned on glass substrates that were irreversibly bonded to microfluidic networks produced in poly-dimethyl-siloxane. Concentration gradients obtained in the fiber containing channels were characterized experimentally and compared with values obtained by computational fluid dynamic simulations. Velocity and shear stress profiles, as well as vortex formation, were calculated to evaluate the influence of fiber pads on fluidic properties. The suitability of the system to support cell attachment and growth was demonstrated with a fibroblast cell line. The potential of the platform was further verified by a functional investigation of neural stem cell alignment in response to orientation of electrospun fibers versus a microfluidic generated chemoattractant gradient of stromal cell-derived factor 1 alpha. The described method is a competitive strategy to create complex microenvironments in vitro that allow detailed studies on the interplay of topography, substrate surface properties, and soluble microenvironment on cellular fate processes. V C 2012 American Institute of Physics. [http://dx

show abstract

The effect of nanofiber-guided cell alignment on the preferential differentiation of neural stem cells

Cited by 241 publications

References 45 publications

The role of the surface on microglia function: implications for central nervous system tissue engineering

The role of the surface on microglia function: implications for central nervous system tissue engineering

A synthetic nanofibrillar matrix promotes in vitro hepatic differentiation of embryonic stem cells and induced pluripotent stem cells

A method to integrate patterned electrospun fibers with microfluidic systems to generate complex microenvironments for cell culture applications

Contact Info

Product

Resources

About