The effect of motility and cell-surface polymers on bacterial attachment

Morisaki, Hisao; Nagai, S.; Ohshima, Hiroyuki; Ikemoto, Eiko; Kogure, Kazuhiro

doi:10.1099/00221287-145-10-2797

Cited by 141 publications

(88 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These have been attributed to the contribution of sedimentation phenomena at lower flow rates [35] and to presence of surface appendages, e.g. flagellum, which may have a positive effect on adhesion, a feature that is also not considered in this model [39]. Li, et al [35] have studied the contribution of sedimentation to mass transport in a PPFC using S. aureus and a glass surface.…”

Section: Discussionmentioning

confidence: 99%

The effects of surface properties on Escherichia coli adhesion are modulated by shear stress

Moreira

Araújo

Miranda

et al. 2014

Colloids and Surfaces B: Biointerfaces

View full text Add to dashboard Cite

The adhesion of Escherichia coli to glass and polydimethylsiloxane (PDMS) at different flow rates (between 1 and 10 ml.s -1 ) was monitored in a parallel plate flow chamber in order to understand the effect of surface properties and hydrodynamic conditions on adhesion. Computational fluid dynamics was used to assess the applicability of this flow chamber in the simulation of the hydrodynamics of relevant biomedical systems. Wall shear stresses between 0.005 and 0.07 Pa were obtained and these are similar to those found in the circulatory, reproductive and urinary systems. Results demonstrate that E.coli adhesion to hydrophobic PDMS and hydrophilic glass surfaces is modulated by shear stress with surface properties having a stronger effect at the lower and highest flow rates tested and with negligible effects at intermediate flow rates. These findings suggest that when expensive materials or coatings are selected to produce biomedical devices, this choice should take into account the physiological hydrodynamic conditions that will occur during the utilization of those devices.

show abstract

Section: Discussionmentioning

confidence: 99%

The effects of surface properties on Escherichia coli adhesion are modulated by shear stress

Moreira

Araújo

Miranda

et al. 2014

Colloids and Surfaces B: Biointerfaces

View full text Add to dashboard Cite

show abstract

“…The above electrophoresis theory of soft particles has been applied to analyze the electrophoretic mobility data of biological colloids such as cells and their model particles [24][25][26][27][28][29][30][31][32][33][34][35]. Ohshima [36,37] proposed a theory of electrophoresis of soft particles in concentrated suspensions.…”

Section: Electrophoretic Mobility Of Soft Particlesmentioning

confidence: 99%

Theory of electrostatics and electrokinetics of soft particles

Ohshima

2009

Science and Technology of Advanced Materials

Self Cite

138

View full text Add to dashboard Cite

We investigate theoretically the electrostatics and electrokinetics of a soft particle, i.e. a hard particle covered with an ion-penetrable surface layer of polyelectrolytes. The electric properties of soft particles in an electrolyte solution, which differ from those of hard particles, are essentially determined by the Donnan potential in the surface layer. In particular, the Donnan potential plays an essential role in the electrostatics and electrokinetics of soft particles. Furthermore, the concept of zeta potential, which is important in the electrokinetics of hard particles, loses its physical meaning in the electrokinetics of soft particles. In this review, we discuss the potential distribution around a soft particle, the electrostatic interaction between two soft particles, and the motion of a soft particle in an electric field.

show abstract

“…It is conceivable that in the contact region, that chargeable groups on the cell wall and Al2O3 surfaces have the same type of charge may be compensated by counterions, thereby preventing the generation of electrostatic repulsion. Kogure et al (1998) and Morisaki et al (1999) demonstrated that negatively charged Vibrio spp. cells could adhere to negatively charged glass surfaces by overcoming the energy barrier due to electrostatic repulsion between the cells and glass surfaces under high ionic strength when the cells were swimming at a high speed and had charged polymers at their surfaces.…”

Section: Introductionmentioning

confidence: 99%

Adhesion of Saccharomyces cerevisiae to Alumina Surfaces and Its Removal by Caustic Alkali Cleaning.

Urano¹,

Nagata²,

Fukuzaki³

2002

Biocontrol Sci.

View full text Add to dashboard Cite

The adhesion of Saccharomyces cerevisiae to hydrophilic alumina (Al203) particles and its removal by caustic alkali cleaning were studied. The surface charge properties of S. cerevisiae cells and Al20 3 particles were determined by potentiometric titration as a function of pH. The cells could adhere spontaneously to Al203 surfaces even though they had the same type of net charge as that of Al203. The maximum adhesion of 44.0mg dry cell/m 2 was observed for the adhesion of negatively charged cells to positively charged Al203 in the pH range of 4.2 to 6.6. Approximately 43% of S. cerevisiae cells adhering to Al203 were desorbed rapidly by rinsing alone and the remainder was removed by subsequent caustic alkali cleaning. The rate of desorption of S. cerevisiae cells during caustic alkali cleaning depended both on the pH and on temperature, and was analyzed by evaluating the first-order desorption rate constant as a measure of cleaning efficiency. After caustic alkali cleaning, whole S. cerevisiae cells were completely removed, but polymeric substances presumably originating from alkali cell lysis remained on the Al203 surface.

show abstract

The effect of motility and cell-surface polymers on bacterial attachment

Cited by 141 publications

References 14 publications

The effects of surface properties on Escherichia coli adhesion are modulated by shear stress

The effects of surface properties on Escherichia coli adhesion are modulated by shear stress

Theory of electrostatics and electrokinetics of soft particles

Adhesion of Saccharomyces cerevisiae to Alumina Surfaces and Its Removal by Caustic Alkali Cleaning.

Contact Info

Product

Resources

About