The effect of monoterpenes on astaxanthin production by a mutant ofPhaffia rhodozyma

Meyer, P. S.; Preez, J. C. du; Dyk, M. S. Van

doi:10.1007/bf01021657

Cited by 14 publications

(8 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Those inhibitors could also a †ect P rhodozyma, and account for the value of Ks found in this work. Phaffia rhodozyma has been found to be inhibited by saponin and monoterpenes (Okagbue and Lewis 1984 ;Meyer et al 1994). Fuchsman (1980) reported that hydroxymethyl furfural, which is present Dry biomass concentration (mean =, values^standard deviation) ; limiting substrate concentra-+, tion (mean values^standard deviation) ; È, predicted values from mathematical modelling.…”

Section: Determination Of Ks Valuementioning

confidence: 99%

“…Several works have been published on the production of astaxanthin by the yeast P rhodozyma in fermentation processes (An et al 1989 ;Fang and Cheng 1993 ;Meyer et al 1993Meyer et al , 1994Meyer and Du Preez 1994a, b ;Hayman et al 1995), including the use of peat hydrolysates as substrate (Martin et al 1993a, b ;Acheampong and Martin 1995). The presence in peat hydrolysates of potential astaxanthin precursors, such as carotenes, was discussed by Martin et al (1993a).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Mathematical model forPhaffia rhodozyma growth using peat hydrolysates as substrate

Vázquez

Martı́n

1998

J. Sci. Food Agric.

View full text Add to dashboard Cite

The potential of peat hydrolysates for the production of astaxanthin from Phaffia rhodozyma yeast biomass in continuous fermentation was studied. Chemostat fermentations of the yeast were performed using peat hydrolysates as substrate. Kinetic parameters were calculated for the growth of the yeast and the biomass productivity was determined using mathematical models. A low maximum specific growth rate (μmax=0·08 h−1) and a high Monod constant (Ks=26·2×103) were obtained for this substrate. The low maintenance energy requirement found, 0·020 g (gh)−1, confirms the aerobic metabolism of the yeast with this substrate. Under selected conditions, a biomass productivity of 0·108 g (lh)−1 and an astaxanthin productivity of 0·046 mg (lh)−1 were obtained. The biomass produced had a high protein and astaxanthin content (490±8 g kg−1 and 0.43±0.07 g kg−1, respectively), indicating that it can be used as a feed additive. © 1998 SCI.

show abstract

Section: Determination Of Ks Valuementioning

confidence: 99%

mentioning

confidence: 99%

Mathematical model forPhaffia rhodozyma growth using peat hydrolysates as substrate

Vázquez

Martı́n

1998

J. Sci. Food Agric.

View full text Add to dashboard Cite

show abstract

“…Among all these micro‐organisms, both the green alga Haematococcus pluvialis and the yeast P. rhodozyma are currently considered as viable sources of astaxanthin for industrial production (An et al. 1989; Fang and Cheng 1993; Meyer and Du‐Preez 1993, 1994; Meyer et al. 1994; Parajó et al.…”

Section: Introductionmentioning

confidence: 99%

Improvement of astaxanthin production by a newly isolated Phaffia rhodozyma mutant with low-energy ion beam implantation

et al. 2008

View full text Add to dashboard Cite

Aims: Isolation, characterization and identification of Phaffia sp. ZJB 00010, and improvement of astaxanthin production with low‐energy ion beam implantation. Methods and Results: A strain of ZJB 00010, capable of producing astaxanthin, was isolated and identified as Phaffia rhodozyma, based on its physiological and biochemical characteristics as well as its internal transcribed spacer (ITS) rDNA gene sequence analysis. With low‐energy ion beam implantation, this wild‐type strain was bred for improving the yield of astaxanthin. After ion beam implantation, the best mutant, E5042, was obtained. The production of astaxanthin in E5042 was 2512 μg g−1 (dry cell weight, DCW), while the wild‐type strain was about 1114 μg g−1 (DCW), an increase of 125·5%. Moreover, the fermentation conditions of mutant E5042 for producing astaxanthin were optimized. The astaxanthin production under the optimized conditions was upscaled and studied in a 50‐l fermentor. Conclusions: A genetically stable mutant strain with high yield of astaxanthin was obtained using low‐energy ion beam implantation. This mutant may be a suitable candidate for the industrial‐scale production of astaxanthin. Significance and Impact of the Study: Astaxanthin production in Phaffia rhodozyma could be fficiently improved by low‐energy ion beam implantation, which is a new technology in the mutant breeding of micro‐organisms. The mutant obtained in this work could potentially be utilized in industrial production of astaxanthin.

show abstract

“…Process optimization, use of high yielding strains, or strain improvement by mutagenesis or genetic engineering are well researched and commonly employed for carotenoid yield improvement especially in Phaffia rhodozyma (see reviews by Lukács et al 2006;Frengova and Beshkova 2009). Precursors, chemicals, or elicitors can also enhance carotenoid production: many natural oils, fatty acids, surfactants, and β-ionone (Ciegler et al 1959), Span-20 a surfactant (Kim et al 1997) and hydrogen peroxide (Jeong et al 1999) have enhanced β-carotene production in Blakslea trispora; lycopene (Johnson and Lewis 1979), β-ionone (Lewis et al 1990), acetic acid , valine , α-pinene (Meyer et al 1994), ethanol (Gu et al 1997), mevalonate (Calo et al 1995), citrate (Flores-Cotera et al 2001), nhexadecane (Liu and Wu 2006a), and hydrogen peroxide (Liu and Wu 2006b) have enhanced astaxanthin or total carotenoid production in P. rhodozyma; organic acids of TCA cycle-enhanced astaxanthin production in algae Rhodopseudomonas sphetoides (Higuchi and Kikuchi 1963), Rhodopseudomonas gelatinosa (Noparatnaraporn et al 1986), Flavobacterium sp. Precursors, chemicals, or elicitors can also enhance carotenoid production: many natural oils, fatty acids, surfactants, and β-ionone (Ciegler et al 1959), Span-20 a surfactant (Kim et al 1997) and hydrogen peroxide (Jeong et al 1999) have enhanced β-carotene production in Blakslea trispora; lycopene (Johnson and Lewis 1979), β-ionone (Lewis et al 1990), acetic acid , valine , α-pinene (Meyer et al 1994), ethanol (Gu et al 1997), mevalonate (Calo et al 1995), citrate (Flores-Cotera et al 2001), nhexadecane (Liu and Wu 2006a), and hydrogen peroxide (Liu and Wu 2006b) have enhanced astaxanthin or total carotenoid production in P. rhodozyma; organic acids of TCA cycle-enhanced astaxanthin production in algae Rhodopseudomonas sphetoides (Higuchi and Kikuchi 1963), Rhodopseudomonas gelatinosa …”

mentioning

confidence: 99%

“…Apart from these routinely used methods, yield enhancement in carotenoid-producing microbes has been achieved by co-culturing with other microbes (Margalith 1993;Buzzini 2001;Dong and Zhao 2004) or by addition of simple nutrients: organic N sources like yeast extract, beef extract, or peptone (Fang and Cheng 1993;An et al 1996;Ramirez et al 2001), or inorganic N sources like urea, KNO 3 , ammonium salts (Fang and Cheng 1993;An et al 1996;Parajo et al 1997;Ni et al 2007). Precursors, chemicals, or elicitors can also enhance carotenoid production: many natural oils, fatty acids, surfactants, and β-ionone (Ciegler et al 1959), Span-20 a surfactant (Kim et al 1997) and hydrogen peroxide (Jeong et al 1999) have enhanced β-carotene production in Blakslea trispora; lycopene (Johnson and Lewis 1979), β-ionone (Lewis et al 1990), acetic acid , valine , α-pinene (Meyer et al 1994), ethanol (Gu et al 1997), mevalonate (Calo et al 1995), citrate (Flores-Cotera et al 2001), nhexadecane (Liu and Wu 2006a), and hydrogen peroxide (Liu and Wu 2006b) have enhanced astaxanthin or total carotenoid production in P. rhodozyma; organic acids of TCA cycle-enhanced astaxanthin production in algae Rhodopseudomonas sphetoides (Higuchi and Kikuchi 1963), Rhodopseudomonas gelatinosa (Noparatnaraporn et al 1986), Flavobacterium sp. (Alcantara and Sanchez 1999), and Chlorella zofingiensis (Chen et al 2009), mevalonate and pyruvate enhanced carotenoid synthesis in Haematococcus pluvialis (Kakizono 1991), and lycopene and β-carotene act as precursors for astaxanthin production in H. pluvialis (Harker and Young 1995); addition of fungal elicitors like Epicoccum nigrum (Echavarri-Erasum and Johnson 2004), Aspergillus sp.…”

mentioning

confidence: 99%

Substrates Influence Stimulatory Effect of Mevalonic Acid on Carotenoid Production in Red Yeasts

Nanjundaswamy

Vadlani

2011

Cereal Chem

View full text Add to dashboard Cite

Cereal Chem. 88(3):310-314Stimulation of carotenogenesis in carotenoid producing red yeasts, algae, or bacteria for enhanced carotenoid production has been achieved by mevalonic acid addition. Recently, carotenoid-enriched feed was produced by Phaffia rhodozyma fermentation of inexpensive animal feeds. Because mevalonate improves carotenoid yield in P. rhodozyma in synthetic medium, this study tested whether a similar enhancement was possible in biobased substrates. Four concentrations, 0, 0.02, 0.04, and 0.1% of mevalonate as a precursor of P. rhodozyma production of astaxanthin and β-carotene were evaluated in five substrates: defatted rice bran, full fat rice bran, wheat bran, corn whole stillage, and synthetic media. Additionally, four concentrations, 0, 0.05, 0.1, and 0.5% of apple pomace and tomato pomace were also evaluated as precursors of carotenogenesis in P. rhodozyma fermentation of corn whole stillage and rice bran. Mevalonic acid, tomato pomace, and apple pomace enhanced carotenoid yields in all substrates in that order. However, the optimal concentration of precursor and the percent increase of carotenoid yield in each substrate were variable, potentially indicating substrate influence on carotenoid stimulation. Among animal feed substrates, mevalonic acid in corn whole stillage resulted in the best astaxanthin yield of 220 μg/g and β-carotene of 904 μg/g. Tomato pomace resulted in 29% astaxanthin and β-carotene enhancement in corn whole stillage, and apple pomace increased β-carotene production by 26% in whole stillage. The use of expensive mevalonate is offset by the inexpensive process of producing carotenoid-enriched DDGS. Optimization of tomato or apple pomace addition may further enhance the carotenoid yields.

show abstract

The effect of monoterpenes on astaxanthin production by a mutant ofPhaffia rhodozyma

Cited by 14 publications

References 9 publications

Mathematical model forPhaffia rhodozyma growth using peat hydrolysates as substrate

Mathematical model forPhaffia rhodozyma growth using peat hydrolysates as substrate

Improvement of astaxanthin production by a newly isolated Phaffia rhodozyma mutant with low-energy ion beam implantation

Substrates Influence Stimulatory Effect of Mevalonic Acid on Carotenoid Production in Red Yeasts

Contact Info

Product

Resources

About