The effect of modelling of hypogravity on postsynaptic acetylcholine receptors and activity of acetylcholinesterase in neuromuscular synapses of fast and slow muscles of rat

Tyapkina, O. V.; Нуруллин, Л. Ф.; Petrov, K. A.; Волков, Е. М.

doi:10.1134/s1990519x14060091

Cited by 1 publication

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“…The data presented here, then, challenge the robustness of pre‐ to postsynaptic coupling in that the relationship between active zones and endplate size was disrupted with unloading as revealed by decreased Bassoon and calcium channel staining dimensions with no adjustment in endplate size. It is likely that this alteration in the relationship between release and binding sites for ACh interferes with neuromuscular transmission contributing to the decrements in neuromuscular function associated with unloading (Tiapkina et al., 2014). Confirmation of this suggestion requires further examination, though.…”

Section: Discussionmentioning

confidence: 99%

Sensitivity of subcellular components of neuromuscular junctions to decreased neuromuscular activity

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et al. 2021

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Muscle unloading imparts subtotal disuse on the neuromuscular system resulting in reduced performance capacity. This loss of function, at least in part, can be attributed to disruptions at the neuromuscular junction (NMJ). However, research has failed to document morphological remodeling of the NMJ with short term muscle unloading. Here, rather than quantifying cellular components of the NMJ, we examined subcellular active zone responses to 2 weeks of unloading in male Wistar rats. It was revealed that in the plantaris, but not the soleus muscles, unloading elicited significant (P ≤ 0.05) decrements in active zone staining as measured by Bassoon, and calcium channel expression. It was also discovered that unloading decreased the area of calcium channels staining relative to active zone areas of staining suggesting potential interference in the ability of calcium influx to trigger the release of vesicles docked at the active zone. Post‐synaptic adaptations of the motor endplate were not evident. This presynaptic subcellular size reduction was not associated with atrophy of the underlying plantaris muscle fibers, although atrophy of the weight‐bearing soleus fibers, where no subcellular remodeling was evident, was noted. These results suggest that the active zone is highly sensitive to alterations in neuromuscular activity, and that morphological adaptation of excitatory and contractile components of the NMJ can occur independently of each other.

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Section: Discussionmentioning

confidence: 99%